library kit Search Results


ultra ii rna sequencing kit  (New England Biolabs)
99
New England Biolabs ultra ii rna sequencing kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Ultra Ii Rna Sequencing Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultra ii rna sequencing kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
ultra ii rna sequencing kit - by Bioz Stars, 2026-03
99/100 stars
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nebnext ultra directional rna library preparation kit  (New England Biolabs)
96
New England Biolabs nebnext ultra directional rna library preparation kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnext Ultra Directional Rna Library Preparation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra directional rna library preparation kit/product/New England Biolabs
Average 96 stars, based on 1 article reviews
nebnext ultra directional rna library preparation kit - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
directional rna library prep kit  (New England Biolabs)
96
New England Biolabs directional rna library prep kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/directional rna library prep kit/product/New England Biolabs
Average 96 stars, based on 1 article reviews
directional rna library prep kit - by Bioz Stars, 2026-03
96/100 stars
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nebnext ultra ii kit  (New England Biolabs)
99
New England Biolabs nebnext ultra ii kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnext Ultra Ii Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra ii kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
nebnext ultra ii kit - by Bioz Stars, 2026-03
99/100 stars
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rna library preparation used nebnext ultra ii rna library preparation kit for illumina  (New England Biolabs)
99
New England Biolabs rna library preparation used nebnext ultra ii rna library preparation kit for illumina
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Rna Library Preparation Used Nebnext Ultra Ii Rna Library Preparation Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna library preparation used nebnext ultra ii rna library preparation kit for illumina/product/New England Biolabs
Average 99 stars, based on 1 article reviews
rna library preparation used nebnext ultra ii rna library preparation kit for illumina - by Bioz Stars, 2026-03
99/100 stars
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nebnext ultra dna library prep kit  (New England Biolabs)
99
New England Biolabs nebnext ultra dna library prep kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnext Ultra Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra dna library prep kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
nebnext ultra dna library prep kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
nebnext ultra dna library prep  (New England Biolabs)
99
New England Biolabs nebnext ultra dna library prep
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnext Ultra Dna Library Prep, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra dna library prep/product/New England Biolabs
Average 99 stars, based on 1 article reviews
nebnext ultra dna library prep - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
nebnextr ultratm directional rna library prep kit for illuminar  (New England Biolabs)
96
New England Biolabs nebnextr ultratm directional rna library prep kit for illuminar
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnextr Ultratm Directional Rna Library Prep Kit For Illuminar, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnextr ultratm directional rna library prep kit for illuminar/product/New England Biolabs
Average 96 stars, based on 1 article reviews
nebnextr ultratm directional rna library prep kit for illuminar - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
nebnext ultra ii fs dna library  (New England Biolabs)
96
New England Biolabs nebnext ultra ii fs dna library
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnext Ultra Ii Fs Dna Library, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra ii fs dna library/product/New England Biolabs
Average 96 stars, based on 1 article reviews
nebnext ultra ii fs dna library - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
nebnext ultra ii dna library prep kit  (New England Biolabs)
99
New England Biolabs nebnext ultra ii dna library prep kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnext Ultra Ii Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra ii dna library prep kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
nebnext ultra ii dna library prep kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
nebnext ultra directional rna library prep kit  (New England Biolabs)
99
New England Biolabs nebnext ultra directional rna library prep kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Nebnext Ultra Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra directional rna library prep kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
nebnext ultra directional rna library prep kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier

Image Search Results


Kraken2 classified read pair counts at the domain-level for (A) RNA shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun sequencing. Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.

Journal: bioRxiv

Article Title: A Novel Tiled-Amplicon Sequencing Assay Targeting the Tomato Brown Rugose Fruit Virus ( ToBRFV ) Genome Reveals Widespread Distribution in Municipal Wastewater Treatment Systems in the Province of Ontario, Canada

doi: 10.1101/2023.11.02.565359

Figure Lengend Snippet: Kraken2 classified read pair counts at the domain-level for (A) RNA shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun sequencing. Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.

Article Snippet: RNA extracts from WWI were prepared for RNA sequencing using the NEB Ultra II RNA Sequencing kit ( New England Biolabs , E7770S), following manufacturer’s recommended protocol, section 5.

Techniques: Virus, Two Tailed Test, Produced, Shotgun Sequencing