Journal: The International journal of developmental biology

Article Title: A screen of kinase inhibitors reveals a potential role of Chk1 in regulating Hydra head regeneration and maintenance.

doi: 10.1387/ijdb.210087yl

Figure Lengend Snippet: Fig. 1. Schematic illustration of the 80 kinase inhibitor screen. Three kinase inhibitors (KIs) were tested per 12-well plate. For each plate, 60 Hydra gastric regions were isolated by removing the head and foot. For each treatment, 15 gastric regions were rinsed with the respective solution (KI #1, KI #2, KI #3, or control) before dividing them into 3 wells (5 gastric regions per well) containing the respective exposure solutions. Control replicates were exposed to 1% DMSO alone and included on every plate.

Article Snippet: The use of kinase inhibitors to identify potential novel regulators of Hydra regeneration To identify novel signaling pathways underlying Hydra regeneration, an unbiased screen of 80 kinase inhibitors (Tocriscreen Kinase Inhibitor Toolbox Cat. No. 3514) was performed.

Techniques: Isolation, Control

Journal: The International journal of developmental biology

Article Title: A screen of kinase inhibitors reveals a potential role of Chk1 in regulating Hydra head regeneration and maintenance.

doi: 10.1387/ijdb.210087yl

Figure Lengend Snippet: Fig. 2. Morphological categorization following exposure to the 80 kinase inhibitors. In total, 4 major morphologies were observed in Hydra following exposure to the 80 kinase inhibitors: (A) complete regeneration, (B) partial regeneration – the presence of shortened tentacles and/or body column, (C) arrested regeneration - no formation of a head and foot, and (D) disintegration - the breakdown of tissues into debris. Scale bar in (A) represents 1000 mm and applies to all panels.

Article Snippet: The use of kinase inhibitors to identify potential novel regulators of Hydra regeneration To identify novel signaling pathways underlying Hydra regeneration, an unbiased screen of 80 kinase inhibitors (Tocriscreen Kinase Inhibitor Toolbox Cat. No. 3514) was performed.

Techniques:

Journal: The International journal of developmental biology

Article Title: A screen of kinase inhibitors reveals a potential role of Chk1 in regulating Hydra head regeneration and maintenance.

doi: 10.1387/ijdb.210087yl

Figure Lengend Snippet: Fig. 3. Drug Exposure Impact of the 80 kinase inhibitors on Hydra regeneration. Kinase inhibitors were grouped by the main signal transduction pathways they are presumed to target. Drug Exposure Impact (DEI) values on regeneration were calculated as the difference in regeneration scores obtained for control and Hydra exposed to 5 mM kinase inhibitor. DEI values for complete regeneration ranged between -1.07 and 1.2; partial regen eration ranged between -4.4 and 0.6; arrested regeneration ranged between -7.67 and -4; and disintegration ranged between -10 and -7.8. Error bars represent ± 2x standard error.

Article Snippet: The use of kinase inhibitors to identify potential novel regulators of Hydra regeneration To identify novel signaling pathways underlying Hydra regeneration, an unbiased screen of 80 kinase inhibitors (Tocriscreen Kinase Inhibitor Toolbox Cat. No. 3514) was performed.

Techniques: Transduction, Control