Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down’s syndrome, preterm delivery and preeclampsia

doi: 10.1186/1477-7827-10-113

Figure Lengend Snippet: Epitope mapping of LHR29 monoclonal antibody. a ) Three recombinant proteins containing 229, 291 or 318 amino acid residues of the LHCGR extracellular domain and C-terminal FLAG-epitope were expressed in CHO cells as described ; b ), recombinant as well as mock transfected extracts (no DNA) were resolved in SDS-PAGE, blotted and probed with anti-FLAG, LHR29 and PG732 mono- and polyclonal antibodies respectively; c ), two monoclonal antibodies (LHR74 and LHR29) and one polyclonal (PG732) were used in western blot analysis of proteins extracted from placental extracts at 12 wks of gestation.

Article Snippet: Purified LHR29 and LHR74 antibodies were initially provided by Dr Hugues Loosfelt (INSERM, France) and subsequently the antibody producing clones were obtained from ATCC (Clone ID CRL-2685 and CRL-2686).

Techniques: Recombinant, FLAG-tag, Transfection, SDS Page, Bioprocessing, Western Blot

Journal: Stem Cell Research & Therapy

Article Title: Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment

doi: 10.1186/s13287-022-02968-z

Figure Lengend Snippet: Identification of MSCs derived from human glioma tissues. A and B Adherent growth patterns and fibroblastic morphology of GA-MSCs cultured in MSC media (× 100, scale bars = 1000 µm). C Immunofluorescence showed that GA-MSCs expressed MSC specific markers CD44 and CD105 (× 400, scale bars = 500 µm)

Article Snippet: The cells were incubated with primary antibodies against CD44 and CD105 (1:100, Proteintech, China), then overnight at 4 °C.

Techniques: Derivative Assay, Cell Culture, Immunofluorescence

Journal: Discover oncology

Article Title: Preclinical efficacy of CBR-5884 against epithelial ovarian cancer cells by targeting the serine synthesis pathway.

doi: 10.1007/s12672-024-01013-0

Figure Lengend Snippet: Fig. 5 CBR-5884-impaired stemness of EOC cells and enhanced chemotherapy sensitivity. A SKOV3 and ID8 cells were treated with CBR- 5884 (50 μM) or dimethyl sulfoxide (DMSO; control) for 5 days, and tumorsphere formation ability was accessed by sphere formation assay. Representative images (left) and quantification data (right) are shown. B Cell cytometry was performed to detect CD44 expression in SKOV3 and ID8 cells after treatment with 30 μM CBR-5884 for 48 h. Representative images (left) and quantification data (right) are shown. C Quanti- tative polymerase chain reaction assay was conducted to measure the mRNA expression levels of stem-related genes in SKOV3 cells, includ- ing ALDH1A1, NANOG, SOX2, EPCAM, and POU5F1 after treatment with 30 μM CBR-5884 for 48 h. D SKOV3 and ID8 cells were treated with DMSO, carboplatin (20 μM), CBR-5884 (30 μM), and carboplatin (20 μM) plus CBR-5884 (30 μM) for 72 h. Cell viability was measured using CCK-8 assay

Article Snippet: To detect CD44-positive cells, a FITC anti-mouse CD44 (IM7; 1:200 dilution, Cat. No. FITC-65117; Proteintech, USA) antibody was used.

Techniques: Control, Tube Formation Assay, Cytometry, Expressing, Polymerase Chain Reaction, CCK-8 Assay