Journal: Cancers

Article Title: Multiparametric Characterization of the DSL-6A/C1 Pancreatic Cancer Model in Rats

doi: 10.3390/cancers16081535

Figure Lengend Snippet: List of antibodies used for the immunological analysis of the DSL-6A/C1 tumors.

Article Snippet: CD11a , PE , REA596 , Human cell line , Miltenyi , 130-109-171.

Techniques:

Journal: Biochimica et biophysica acta

Article Title: Facilitation of Orai3 targeting and store-operated function by Orai1.

doi: 10.1016/j.bbamcr.2015.03.007

Figure Lengend Snippet: Fig. 4. Effect of Orai1 on translocation of Orai2 and Orai3 to the immune synpase. (a) Confocal fluorescence images of Jurkat E6.1expressing HA–Orai1 (I, II), HA–Orai2 (III, IV) or HA–Orai3 (V, VI) cell lines transfected with non-targeting RNA (left panel) or Orai1 siRNA (right panel) seeded on coverslips without (no IS) or with (IS) precoating with anti-CD3 and anti-LFA1 antibodies to mimic an immune synapse. Cells were stained with Alexa-488 coupled anti-HA antibody and the shown images depict (from left to right) a stack of a representative cell within the coverslip-near region (IS stack), within the cell in an intermediate focal plane (intermediate stack), an X–Y (XY MIP) and a Z–Y maximum intensity projections (ZY MIP). Average HA–Orai1, 2 or 3 translocated to the IS as a percent of sum of fluorescence of the first 6 stacks to the total sum of fluorescence of all stacks of the corresponding cell, was calculated from cells transfected with non-targeting RNA (b) and 3′UTR siRNA of Orai1 in (c). n = 98–102 for each analysed condition.

Article Snippet: To mimic an immune synapse (IS), coverslips were coated with poly-lysine (0.001%) for 30 min then by a mixture of anti-human CD3 (2 μg/μl, Diaclone) and anti LFA1 (1 μg/ml, Acris) antibodies in PBS for 3 h at 37 °C or with PBS for the no IS control.

Techniques: Translocation Assay, Transfection, Staining

Journal: Science Advances

Article Title: The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration

doi: 10.1126/sciadv.abl5942

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: APC anti-human CD11a , Mouse IgG1 , Miltenyi Biotec 130-127-294 , 1:50.

Techniques: Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury

doi: 10.3390/ijms22073551

Figure Lengend Snippet: Antibodies used for analysis of EV surface markers using vesicle flow cytometry.

Article Snippet: CD18 , M18/2 , APC , 130-104-019 , Miltenyi Biotec.

Techniques: Cytometry