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Image Search Results
Journal: Molecular Therapy. Methods & Clinical Development
Article Title: Increased functional potency of multi-edited CAR-T cells manufactured by a non-viral transfection system
doi: 10.1016/j.omtm.2024.101389
Figure Lengend Snippet: Analysis of TSCM phenotype over time (A and B) CD4 + (A) and (B) CD8 + T cells were expanded for 0–4 days post-transfection in a 24-well G-Rex plate and stimulation with TransACT and IL-2 ( N = 3 donors in duplicate). T SCM phenotype (CD45RA + CD45RO − CD62L + CCR7 + CD95 + ) was measured by flow cytometry, and a comparison of results for Solupore and electroporation TKO transfected T cells and untransfected control cells is shown. (C and D) CAR-T cells were generated via lentiviral transduction, and the T SCM phenotype of CD4 + and CD8 + CAR-T cells was analyzed 24 h post-transduction (pre-cryopreservation; C) and (D) post-thaw. n = 3 donors, paired t test, p = 0.0396 (C). Data represented as mean +/- SD.
Article Snippet: T cells were isolated from the fresh Leukopak, within 24 h of collection, via positive selection using the Straight from
Techniques: Transfection, Flow Cytometry, Comparison, Electroporation, Control, Generated, Transduction
Journal: Molecular Therapy. Methods & Clinical Development
Article Title: Increased functional potency of multi-edited CAR-T cells manufactured by a non-viral transfection system
doi: 10.1016/j.omtm.2024.101389
Figure Lengend Snippet: Flow cytometry antibodies
Article Snippet: T cells were isolated from the fresh Leukopak, within 24 h of collection, via positive selection using the Straight from
Techniques: Flow Cytometry, In Vivo
Journal: Cell Stem Cell
Article Title: Choice of template delivery mitigates the genotoxic risk and adverse impact of editing in human hematopoietic stem cells
doi: 10.1016/j.stem.2022.09.001
Figure Lengend Snippet:
Article Snippet:
Techniques: Staining, Recombinant, Electroporation, Purification, cDNA Synthesis, Sequencing, Software, Real-time Polymerase Chain Reaction