leptomycin b Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Millipore leptomycin b
    Consequences of siRNA-induced XPO5 downregulation in the metastatic melanoma cell lines Mel Im and Hmb2 A. Relative expression of genes involved in miRNA processing and mRNA transport. A significant knockdown of XPO5 in melanoma cell lines did not alter the gene expression of Dicer, DGCR8, AGO2, Drosha or XPO1 compared with control treatment (siCtrl) (n=7). B. Immunofluorescence staining and C. Western blot analysis of XPO5 showed a strong downregulation of XPO5in Mel Im and Hmb2 cells after siXPO5 treatment. D. Relative levels of mature miRNAs after XPO5 knockdown compared with siCtrl (n=4). Except for miR-373 and miR-497*, the levels of all tested miRNAs were significantly lower compared with those of the siCtrl treatment. E-H. Relative miRNA levels after XPO1 inhibition (n=4). The levels of (E) miR-373 and (F) miR-497* were significantly lower after XPO1 inhibition via <t>leptomycin</t> B (LMB) compared with control-treated cells (Ctrl). The levels of (G) miR-30b* and (H) miR-106b were not affected by XPO1 inhibition.
    Leptomycin B, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptomycin b/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leptomycin b - by Bioz Stars, 2022-09
    96/100 stars
      Buy from Supplier

    86
    Millipore leptomycine b
    Consequences of siRNA-induced XPO5 downregulation in the metastatic melanoma cell lines Mel Im and Hmb2 A. Relative expression of genes involved in miRNA processing and mRNA transport. A significant knockdown of XPO5 in melanoma cell lines did not alter the gene expression of Dicer, DGCR8, AGO2, Drosha or XPO1 compared with control treatment (siCtrl) (n=7). B. Immunofluorescence staining and C. Western blot analysis of XPO5 showed a strong downregulation of XPO5in Mel Im and Hmb2 cells after siXPO5 treatment. D. Relative levels of mature miRNAs after XPO5 knockdown compared with siCtrl (n=4). Except for miR-373 and miR-497*, the levels of all tested miRNAs were significantly lower compared with those of the siCtrl treatment. E-H. Relative miRNA levels after XPO1 inhibition (n=4). The levels of (E) miR-373 and (F) miR-497* were significantly lower after XPO1 inhibition via <t>leptomycin</t> B (LMB) compared with control-treated cells (Ctrl). The levels of (G) miR-30b* and (H) miR-106b were not affected by XPO1 inhibition.
    Leptomycine B, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptomycine b/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leptomycine b - by Bioz Stars, 2022-09
    86/100 stars
      Buy from Supplier

    86
    LC Laboratories leptomycin b
    Nuclear accumulation of ESR1 prevents proteasomal degradation in RB1 kd1 cells (A) MCF7 cells were treated with leptomycin B, estradiol or a combination for indicated time. Estradiol allows the translocation of ESR1 in the nucleus and <t>Leptomycin</t> B prevents the nuclear export. After 3 or 6 hours of treatment, the RB1 kd1 cells express the same level of ESR1 as scrambled cells. (B) T47D cells were treated with CHX, estradiol, or a combination for 3 hours. CHX allows nuclear accumulation of ESR1. After CHX plus estradiol treatment, the RB1 kd1 cells express the same level of ESR1 as scrambled cells.
    Leptomycin B, supplied by LC Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptomycin b/product/LC Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leptomycin b - by Bioz Stars, 2022-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Consequences of siRNA-induced XPO5 downregulation in the metastatic melanoma cell lines Mel Im and Hmb2 A. Relative expression of genes involved in miRNA processing and mRNA transport. A significant knockdown of XPO5 in melanoma cell lines did not alter the gene expression of Dicer, DGCR8, AGO2, Drosha or XPO1 compared with control treatment (siCtrl) (n=7). B. Immunofluorescence staining and C. Western blot analysis of XPO5 showed a strong downregulation of XPO5in Mel Im and Hmb2 cells after siXPO5 treatment. D. Relative levels of mature miRNAs after XPO5 knockdown compared with siCtrl (n=4). Except for miR-373 and miR-497*, the levels of all tested miRNAs were significantly lower compared with those of the siCtrl treatment. E-H. Relative miRNA levels after XPO1 inhibition (n=4). The levels of (E) miR-373 and (F) miR-497* were significantly lower after XPO1 inhibition via leptomycin B (LMB) compared with control-treated cells (Ctrl). The levels of (G) miR-30b* and (H) miR-106b were not affected by XPO1 inhibition.

    Journal: Oncotarget

    Article Title: Induction of exportin-5 expression during melanoma development supports the cellular behavior of human malignant melanoma cells

    doi: 10.18632/oncotarget.11410

    Figure Lengend Snippet: Consequences of siRNA-induced XPO5 downregulation in the metastatic melanoma cell lines Mel Im and Hmb2 A. Relative expression of genes involved in miRNA processing and mRNA transport. A significant knockdown of XPO5 in melanoma cell lines did not alter the gene expression of Dicer, DGCR8, AGO2, Drosha or XPO1 compared with control treatment (siCtrl) (n=7). B. Immunofluorescence staining and C. Western blot analysis of XPO5 showed a strong downregulation of XPO5in Mel Im and Hmb2 cells after siXPO5 treatment. D. Relative levels of mature miRNAs after XPO5 knockdown compared with siCtrl (n=4). Except for miR-373 and miR-497*, the levels of all tested miRNAs were significantly lower compared with those of the siCtrl treatment. E-H. Relative miRNA levels after XPO1 inhibition (n=4). The levels of (E) miR-373 and (F) miR-497* were significantly lower after XPO1 inhibition via leptomycin B (LMB) compared with control-treated cells (Ctrl). The levels of (G) miR-30b* and (H) miR-106b were not affected by XPO1 inhibition.

    Article Snippet: After 4 h, the cells were treated with 20 mM of the PI3 kinase inhibitor LY-294002, 20 mM of Wortmannin (both Sigma-Aldrich, St Louis, MO, USA) or a control substance for 16 h. For the inhibition of XPO1, cells were incubated with 10 ng/ml of leptomycin B (LMB, Sigma-Aldrich) for 20 h. For MEK inhibition, cells were treated with 10 μM PD98059 or 10 μM U0126 (Merck Millipore, Billerica, MA, USA) for 48 h. RNA polymerase was inhibited using 5 μM α-amanitin (AppliChem, Darmstadt, Germany).

    Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Inhibition

    Nuclear import of PLD1 is not due to inhibition of nuclear export. A , HEK293 cells were left untreated or treated with LMB (leptomycin B, 5 ng/ml) for 4 h, fixed, permeabilized, and stained with an FITC-conjugated antibody specific for MDM2 ( green ) and

    Journal: The Journal of Biological Chemistry

    Article Title: Nuclear Localization of Phospholipase D1 Mediates the Activation of Nuclear Protein Kinase C? and Extracellular Signal-regulated Kinase Signaling Pathways *

    doi: 10.1074/jbc.M110.162602

    Figure Lengend Snippet: Nuclear import of PLD1 is not due to inhibition of nuclear export. A , HEK293 cells were left untreated or treated with LMB (leptomycin B, 5 ng/ml) for 4 h, fixed, permeabilized, and stained with an FITC-conjugated antibody specific for MDM2 ( green ) and

    Article Snippet: Leptomycin B (LMB), phorbol myristate acetate and platelet-derived growth factor (PDGF) were from Calbiochem.

    Techniques: Inhibition, Staining

    Nuclear accumulation of ESR1 prevents proteasomal degradation in RB1 kd1 cells (A) MCF7 cells were treated with leptomycin B, estradiol or a combination for indicated time. Estradiol allows the translocation of ESR1 in the nucleus and Leptomycin B prevents the nuclear export. After 3 or 6 hours of treatment, the RB1 kd1 cells express the same level of ESR1 as scrambled cells. (B) T47D cells were treated with CHX, estradiol, or a combination for 3 hours. CHX allows nuclear accumulation of ESR1. After CHX plus estradiol treatment, the RB1 kd1 cells express the same level of ESR1 as scrambled cells.

    Journal: Oncotarget

    Article Title: pRb controls Estrogen Receptor alpha protein stability and activity

    doi:

    Figure Lengend Snippet: Nuclear accumulation of ESR1 prevents proteasomal degradation in RB1 kd1 cells (A) MCF7 cells were treated with leptomycin B, estradiol or a combination for indicated time. Estradiol allows the translocation of ESR1 in the nucleus and Leptomycin B prevents the nuclear export. After 3 or 6 hours of treatment, the RB1 kd1 cells express the same level of ESR1 as scrambled cells. (B) T47D cells were treated with CHX, estradiol, or a combination for 3 hours. CHX allows nuclear accumulation of ESR1. After CHX plus estradiol treatment, the RB1 kd1 cells express the same level of ESR1 as scrambled cells.

    Article Snippet: MG-132 (10012628) from Cayman Chemical; Bortezomib (B-1408) and Leptomycin B (L-6100) from LC Laboratories; Cycloheximide (c4859) from Sigma; and (Z)-4-Hydroxytamoxifen from Enzo Life Sciences.

    Techniques: Translocation Assay