Journal: Stem Cell Research & Therapy

Article Title: Human umbilical cord mesenchymal stem cell-derived exosomal miR-27b attenuates subretinal fibrosis via suppressing epithelial–mesenchymal transition by targeting HOXC6

doi: 10.1186/s13287-020-02064-0

Figure Lengend Snippet: hucMSC-Exo treatment had less fibrosis development in vivo. a Study design. b The RPE–Bruch membrane complex was disrupted, the normal arrangement of the inner nuclear layer was lost and neovascular hide beneath the subretinal space 7 days after the laser burn. Compared with the PBS group, the number of collagen fibers reduced (blue) in the group treated with hucMSC-derived exosomes 35 days after laser photocoagulation. c Choroidal neovascularization (CNV; dyed with isolectin B4) and fibrosis (dyed with collagen type I) were recorded 7, 21, and 35 days after laser burn in the PBS and hucMSC-Exo groups. d Mean volume of the CNV and fibrosis 7, 21, and 35 days after laser burn

Article Snippet: Then, the RPE–choroid complexes were washed, blocked with 5% goat serum albumin and 0.3% Triton X-100, and then incubated with FITC-labeled isolectin-B4 (IB4) (Vector Laboratories, 1:100) (for evaluating CNV) and collagen type I antibody (ab34710, Abcam, dilution 1:100) (for evaluating subretinal fibrosis) at 4 °C overnight.

Techniques: In Vivo, Derivative Assay

Journal: Sensors (Basel, Switzerland)

Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker

doi: 10.3390/s24041128

Figure Lengend Snippet: Summary of results obtained using lectins to bind (+) or not to bind (−) to exosomes immobilised at SPR sensor 1 chip.

Article Snippet: To regenerate the biorecognition surface and remove the attached lectins, elution solutions from Vector Laboratories (Burlingame, CA, USA) were purchased, i.e., glycoprotein-eluting solution for mannose/glucose binding lectins (ES-1100-100); glycoprotein-eluting solution for galactose/GalNAc binding lectins (ES-2100-100); glycoprotein-eluting solution for fucose/arabinose binding lectins (ES3100-100); glycoprotein eluting solution for GlcNAc/Chitin binding lectins (ES-5100-100); glycoprotein-eluting solution for sialic acid binding lectins (ES-7100-100).

Techniques: Control

Journal: Sensors (Basel, Switzerland)

Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker

doi: 10.3390/s24041128

Figure Lengend Snippet: SPR sensorgram showing interaction of immobilised 22Rv1-derived exosomes with SNA and MAAII (both sialic acid-specific) lectins. Higher analytical sensitivities and lower limits of detection (LoDs) were achieved for carcinoma exosomes ( upper row ). SCK SPR sensorgrams showing interaction of SNA lectin with control ( lower left ) and carcinoma ( lower right ) exosomes. The analyte was injected into the system at concentrations of 18.75, 37.5, 75, 150 and 300 nM.

Article Snippet: To regenerate the biorecognition surface and remove the attached lectins, elution solutions from Vector Laboratories (Burlingame, CA, USA) were purchased, i.e., glycoprotein-eluting solution for mannose/glucose binding lectins (ES-1100-100); glycoprotein-eluting solution for galactose/GalNAc binding lectins (ES-2100-100); glycoprotein-eluting solution for fucose/arabinose binding lectins (ES3100-100); glycoprotein eluting solution for GlcNAc/Chitin binding lectins (ES-5100-100); glycoprotein-eluting solution for sialic acid binding lectins (ES-7100-100).

Techniques: Derivative Assay, Control, Injection

Journal: Sensors (Basel, Switzerland)

Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker

doi: 10.3390/s24041128

Figure Lengend Snippet: Summary of results obtained using lectins to bind (+) or not to bind (−) to exosomes immobilised at SPR sensor 1 chip.

Article Snippet: To regenerate the biorecognition surface and remove the attached lectins, elution solutions from Vector Laboratories (Burlingame, CA, USA) were purchased, i.e., glycoprotein-eluting solution for mannose/glucose binding lectins (ES-1100-100); glycoprotein-eluting solution for galactose/GalNAc binding lectins (ES-2100-100); glycoprotein-eluting solution for fucose/arabinose binding lectins (ES3100-100); glycoprotein eluting solution for GlcNAc/Chitin binding lectins (ES-5100-100); glycoprotein-eluting solution for sialic acid binding lectins (ES-7100-100).

Techniques: Control

Journal: Sensors (Basel, Switzerland)

Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker

doi: 10.3390/s24041128

Figure Lengend Snippet: SPR sensorgram showing interaction of immobilised 22Rv1-derived exosomes with SNA and MAAII (both sialic acid-specific) lectins. Higher analytical sensitivities and lower limits of detection (LoDs) were achieved for carcinoma exosomes ( upper row ). SCK SPR sensorgrams showing interaction of SNA lectin with control ( lower left ) and carcinoma ( lower right ) exosomes. The analyte was injected into the system at concentrations of 18.75, 37.5, 75, 150 and 300 nM.

Article Snippet: To regenerate the biorecognition surface and remove the attached lectins, elution solutions from Vector Laboratories (Burlingame, CA, USA) were purchased, i.e., glycoprotein-eluting solution for mannose/glucose binding lectins (ES-1100-100); glycoprotein-eluting solution for galactose/GalNAc binding lectins (ES-2100-100); glycoprotein-eluting solution for fucose/arabinose binding lectins (ES3100-100); glycoprotein eluting solution for GlcNAc/Chitin binding lectins (ES-5100-100); glycoprotein-eluting solution for sialic acid binding lectins (ES-7100-100).

Techniques: Derivative Assay, Control, Injection

Journal: Sensors (Basel, Switzerland)

Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker

doi: 10.3390/s24041128

Figure Lengend Snippet: Summary of results obtained using lectins to bind (+) or not to bind (−) to exosomes immobilised at SPR sensor 1 chip.

Article Snippet: To regenerate the biorecognition surface and remove the attached lectins, elution solutions from Vector Laboratories (Burlingame, CA, USA) were purchased, i.e., glycoprotein-eluting solution for mannose/glucose binding lectins (ES-1100-100); glycoprotein-eluting solution for galactose/GalNAc binding lectins (ES-2100-100); glycoprotein-eluting solution for fucose/arabinose binding lectins (ES3100-100); glycoprotein eluting solution for GlcNAc/Chitin binding lectins (ES-5100-100); glycoprotein-eluting solution for sialic acid binding lectins (ES-7100-100).

Techniques: Control

Journal: Sensors (Basel, Switzerland)

Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker

doi: 10.3390/s24041128

Figure Lengend Snippet: SPR sensorgram showing interaction of immobilised 22Rv1-derived exosomes with SNA and MAAII (both sialic acid-specific) lectins. Higher analytical sensitivities and lower limits of detection (LoDs) were achieved for carcinoma exosomes ( upper row ). SCK SPR sensorgrams showing interaction of SNA lectin with control ( lower left ) and carcinoma ( lower right ) exosomes. The analyte was injected into the system at concentrations of 18.75, 37.5, 75, 150 and 300 nM.

Article Snippet: To regenerate the biorecognition surface and remove the attached lectins, elution solutions from Vector Laboratories (Burlingame, CA, USA) were purchased, i.e., glycoprotein-eluting solution for mannose/glucose binding lectins (ES-1100-100); glycoprotein-eluting solution for galactose/GalNAc binding lectins (ES-2100-100); glycoprotein-eluting solution for fucose/arabinose binding lectins (ES3100-100); glycoprotein eluting solution for GlcNAc/Chitin binding lectins (ES-5100-100); glycoprotein-eluting solution for sialic acid binding lectins (ES-7100-100).

Techniques: Derivative Assay, Control, Injection