Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: ( A ) Different parts of seeds dissected for DAS-ELISA; a. Seed coat, b. Endosperm. c. Embryo. ( B ) Detection of ToLCNDV in DAS-ELISA-positive embryo samples of H2 seeds from market; Lane-M—1 Kb Ladder, L1–L16—H2 Embryo samples, Lane M—1 Kb Ladder, L17, 18—H2 Embryo samples; L16, 19—PC.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: Detection of begomoviruses in ELISA-positive embryo samples of seeds through PCR using specific primers.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: ( A ) Severe mosaic—BgYMV alone ( B ) Severe Mosaic and upward rolling—ToLCNDV alone ( C ) Mild mosaic and upward curling—ToLCNDV and BgYMV ( D ) Malformed fruits from plant with both viruses.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques:

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: Detection of ToLCNDV in DAS-ELISA-positive embryo samples of H2 seeds from field-collected seeds. M—1 Kb Ladder, L1–L5—H2 Embryo samples; L6—PC.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: Detection of begomoviruses in bitter gourd grow-out test plants in glasshouse through PCR.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Transmission Assay

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: ( A ) Detection of ToLCNDV in grow-out test leaf samples from microplot I. M—1 Kb ladder; L1 to 13 grow-out test leaf samples—ToLCNDV, L14—PC. ( B ) Detection of BgYMV in grow-out test plants in microplot I. M—1 Kb ladder; L1 to 6—Grow-out test leaf samples-BgYMV, L7—PC.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques:

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: Detection of begomoviruses in bitter gourd grow-out test plants (leaf samples) in microplot I.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Transmission Assay, Derivative Assay, Infection

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: Whitefly transmission of ToLCNDV from microplot grow-out test plants. ( A ) Whiteflies feeding on symptomatic leaves −24 h (AAP); ( B ) Whiteflies feeding on Asymptomatic leaves −24 h (AAP); ( C ) Symptom expressed in plants transmitted from symptomatic plants ( D ) Symptom expressed in plants transmitted from asymptomatic plants.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Transmission Assay

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: Transmission of the begomovirus (ToLCNDV) to bitter gourd seedlings using Bemisia tabaci .

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Transmission Assay

Journal: Plants

Article Title: Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

doi: 10.3390/plants12061396

Figure Lengend Snippet: Extent of spread of begomoviruses from seedling borne inoculum through the vector B. tabaci in microplot II.

Article Snippet: The experiment was conducted in Nunc-Immuno 96-well microtiter plates (Sigma-Aldrich), with ToLCNDV DAS-ELISA kit from DSMZ Germany (AS-1109).

Techniques: Plasmid Preparation, Transmission Assay