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  • 99
    Thermo Fisher tracefinder software 4 1 quantitative analysis
    Tracefinder Software 4 1 Quantitative Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore lc ms grade acetonitrile
    Lc Ms Grade Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies lc ms ms analysis
    Physiological relevance of AKG (A-D). The serum concentration of succinic acid (A), malic (B), hypoxanthine (C) and xanthine (D) in mice. Chow fed male C57BL/6 mice (10 weeks) were divided into three groups receiving non-exercise, endurance exercise (treadmill, 10 meters/minute, increased by 2 meters/minute every two minutes to exhaustion) or resistance exercise (ladder climbing for 40 min) (n = 8 per group). (E). Two tailed Pearson’s correlation coefficient <t>analysis</t> of plasma AKG level and the running distance of wheels. Chow fed male C57BL/6 mice (10 weeks) received one-day free access to running wheel. (n = 10 per group). (F). Two tailed Pearson’s correlation coefficient analysis of plasma AKG level and the ladder climbing time. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise (ladder climbing for 40 min). (n = 10 per group). (F). Serum AKG level after electric stimulation. Electric stimulation was performed in unilateral gastrocnemius for 40 min (1 <t>ms</t> width/50 Hz, 10 times, each time for 4 min, resting for 2 min between stimulates) in vivo in 10 weeks male C57BL/6 mice fed with chow diet (n = 8-9 per group). (G). Serum lactate concentration. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise for 40 min. The serum lactate concentration was test before and immediately after exercise. (n = 10 per group). (H). Two tailed Pearson’s correlation coefficient analysis of human plasma AKG level and BMI, hip circumference (HCF), waist circumference (WCF), fat mass, body weight, neck circumference (NCF), and height. *p≤0.05, ** p≤0.01, *** p≤0.001 indicate significant correlation between human plasma AKG level and BMI, HCF, WCF, fat mass and body weight. Results are presented as mean ± SEM. In (A-D) and (G-H), ∗p≤0.05 by one-way ANOVA followed by post hoc Turkey’s tests.
    Lc Ms Ms Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 2215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation lc ms ms analysis
    Physiological relevance of AKG (A-D). The serum concentration of succinic acid (A), malic (B), hypoxanthine (C) and xanthine (D) in mice. Chow fed male C57BL/6 mice (10 weeks) were divided into three groups receiving non-exercise, endurance exercise (treadmill, 10 meters/minute, increased by 2 meters/minute every two minutes to exhaustion) or resistance exercise (ladder climbing for 40 min) (n = 8 per group). (E). Two tailed Pearson’s correlation coefficient <t>analysis</t> of plasma AKG level and the running distance of wheels. Chow fed male C57BL/6 mice (10 weeks) received one-day free access to running wheel. (n = 10 per group). (F). Two tailed Pearson’s correlation coefficient analysis of plasma AKG level and the ladder climbing time. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise (ladder climbing for 40 min). (n = 10 per group). (F). Serum AKG level after electric stimulation. Electric stimulation was performed in unilateral gastrocnemius for 40 min (1 <t>ms</t> width/50 Hz, 10 times, each time for 4 min, resting for 2 min between stimulates) in vivo in 10 weeks male C57BL/6 mice fed with chow diet (n = 8-9 per group). (G). Serum lactate concentration. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise for 40 min. The serum lactate concentration was test before and immediately after exercise. (n = 10 per group). (H). Two tailed Pearson’s correlation coefficient analysis of human plasma AKG level and BMI, hip circumference (HCF), waist circumference (WCF), fat mass, body weight, neck circumference (NCF), and height. *p≤0.05, ** p≤0.01, *** p≤0.001 indicate significant correlation between human plasma AKG level and BMI, HCF, WCF, fat mass and body weight. Results are presented as mean ± SEM. In (A-D) and (G-H), ∗p≤0.05 by one-way ANOVA followed by post hoc Turkey’s tests.
    Lc Ms Ms Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation lc ms ms system
    Physiological relevance of AKG (A-D). The serum concentration of succinic acid (A), malic (B), hypoxanthine (C) and xanthine (D) in mice. Chow fed male C57BL/6 mice (10 weeks) were divided into three groups receiving non-exercise, endurance exercise (treadmill, 10 meters/minute, increased by 2 meters/minute every two minutes to exhaustion) or resistance exercise (ladder climbing for 40 min) (n = 8 per group). (E). Two tailed Pearson’s correlation coefficient <t>analysis</t> of plasma AKG level and the running distance of wheels. Chow fed male C57BL/6 mice (10 weeks) received one-day free access to running wheel. (n = 10 per group). (F). Two tailed Pearson’s correlation coefficient analysis of plasma AKG level and the ladder climbing time. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise (ladder climbing for 40 min). (n = 10 per group). (F). Serum AKG level after electric stimulation. Electric stimulation was performed in unilateral gastrocnemius for 40 min (1 <t>ms</t> width/50 Hz, 10 times, each time for 4 min, resting for 2 min between stimulates) in vivo in 10 weeks male C57BL/6 mice fed with chow diet (n = 8-9 per group). (G). Serum lactate concentration. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise for 40 min. The serum lactate concentration was test before and immediately after exercise. (n = 10 per group). (H). Two tailed Pearson’s correlation coefficient analysis of human plasma AKG level and BMI, hip circumference (HCF), waist circumference (WCF), fat mass, body weight, neck circumference (NCF), and height. *p≤0.05, ** p≤0.01, *** p≤0.001 indicate significant correlation between human plasma AKG level and BMI, HCF, WCF, fat mass and body weight. Results are presented as mean ± SEM. In (A-D) and (G-H), ∗p≤0.05 by one-way ANOVA followed by post hoc Turkey’s tests.
    Lc Ms Ms System, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 94/100, based on 997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nonlinear Dynamics progenesis lc ms software
    Estimated power versus putative type 1 error rate (α) with validation <t>(Progenesis</t> PPA measurements). a , power results for Progenesis PPA measurement data (as in ). At α = 0.05, the estimated power of ProPCA is 0.60, the estimated
    Progenesis Lc Ms Software, supplied by Nonlinear Dynamics, used in various techniques. Bioz Stars score: 91/100, based on 653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nonlinear Dynamics progenesis lc ms
    Spike-in experiment to evaluate analysis of LC-MS data generated by a label-free LC-MS method . The experiment design involves two groups of samples: (i) serum samples with spike-in peptides, and (ii) serum samples alone. LC-MS data were generated by UPLC-QTOF mass spectrometer. Four software tools (msInspect, MZmine 2, <t>Progenesis</t> LC-MS, and XCMS) were applied for LC-MS data analysis to evaluate workflows implemented.
    Progenesis Lc Ms, supplied by Nonlinear Dynamics, used in various techniques. Bioz Stars score: 94/100, based on 706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Matrix Science lc ms ms data
    Spike-in experiment to evaluate analysis of LC-MS data generated by a label-free LC-MS method . The experiment design involves two groups of samples: (i) serum samples with spike-in peptides, and (ii) serum samples alone. LC-MS data were generated by UPLC-QTOF mass spectrometer. Four software tools (msInspect, MZmine 2, <t>Progenesis</t> LC-MS, and XCMS) were applied for LC-MS data analysis to evaluate workflows implemented.
    Lc Ms Ms Data, supplied by Matrix Science, used in various techniques. Bioz Stars score: 94/100, based on 509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific lc ms grade water
    Spike-in experiment to evaluate analysis of LC-MS data generated by a label-free LC-MS method . The experiment design involves two groups of samples: (i) serum samples with spike-in peptides, and (ii) serum samples alone. LC-MS data were generated by UPLC-QTOF mass spectrometer. Four software tools (msInspect, MZmine 2, <t>Progenesis</t> LC-MS, and XCMS) were applied for LC-MS data analysis to evaluate workflows implemented.
    Lc Ms Grade Water, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 276 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nano lc ms ms
    Spike-in experiment to evaluate analysis of LC-MS data generated by a label-free LC-MS method . The experiment design involves two groups of samples: (i) serum samples with spike-in peptides, and (ii) serum samples alone. LC-MS data were generated by UPLC-QTOF mass spectrometer. Four software tools (msInspect, MZmine 2, <t>Progenesis</t> LC-MS, and XCMS) were applied for LC-MS data analysis to evaluate workflows implemented.
    Nano Lc Ms Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 661 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation lcms it tof
    Representative chromatograms of RR extract analyzed on <t>LCMS-IT-TOF.</t> (A) UV chromatogram at 210 nm; (B) Total negative iron chromatogram; (C) Total positive iron chromatogram.
    Lcms It Tof, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 91/100, based on 564 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 6520 accurate mass q tof lc ms
    Representative chromatograms of RR extract analyzed on <t>LCMS-IT-TOF.</t> (A) UV chromatogram at 210 nm; (B) Total negative iron chromatogram; (C) Total positive iron chromatogram.
    6520 Accurate Mass Q Tof Lc Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation lcms it tof mass spectrometer
    Representative chromatograms of RR extract analyzed on <t>LCMS-IT-TOF.</t> (A) UV chromatogram at 210 nm; (B) Total negative iron chromatogram; (C) Total positive iron chromatogram.
    Lcms It Tof Mass Spectrometer, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 91/100, based on 433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific lc ms grade acetonitrile
    Representative chromatograms of RR extract analyzed on <t>LCMS-IT-TOF.</t> (A) UV chromatogram at 210 nm; (B) Total negative iron chromatogram; (C) Total positive iron chromatogram.
    Lc Ms Grade Acetonitrile, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation lcms 8050 triple quadrupole mass spectrometer
    Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu <t>LCMS-8050</t> triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP
    Lcms 8050 Triple Quadrupole Mass Spectrometer, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 89/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation shimadzu lcms 2020
    Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu <t>LCMS-8050</t> triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP
    Shimadzu Lcms 2020, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies 6530 accurate mass q tof lc ms
    Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu <t>LCMS-8050</t> triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP
    6530 Accurate Mass Q Tof Lc Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific lc ms grade
    Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu <t>LCMS-8050</t> triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP
    Lc Ms Grade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies chromatography mass spectrometry lc ms
    Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu <t>LCMS-8050</t> triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP
    Chromatography Mass Spectrometry Lc Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Physiological relevance of AKG (A-D). The serum concentration of succinic acid (A), malic (B), hypoxanthine (C) and xanthine (D) in mice. Chow fed male C57BL/6 mice (10 weeks) were divided into three groups receiving non-exercise, endurance exercise (treadmill, 10 meters/minute, increased by 2 meters/minute every two minutes to exhaustion) or resistance exercise (ladder climbing for 40 min) (n = 8 per group). (E). Two tailed Pearson’s correlation coefficient analysis of plasma AKG level and the running distance of wheels. Chow fed male C57BL/6 mice (10 weeks) received one-day free access to running wheel. (n = 10 per group). (F). Two tailed Pearson’s correlation coefficient analysis of plasma AKG level and the ladder climbing time. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise (ladder climbing for 40 min). (n = 10 per group). (F). Serum AKG level after electric stimulation. Electric stimulation was performed in unilateral gastrocnemius for 40 min (1 ms width/50 Hz, 10 times, each time for 4 min, resting for 2 min between stimulates) in vivo in 10 weeks male C57BL/6 mice fed with chow diet (n = 8-9 per group). (G). Serum lactate concentration. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise for 40 min. The serum lactate concentration was test before and immediately after exercise. (n = 10 per group). (H). Two tailed Pearson’s correlation coefficient analysis of human plasma AKG level and BMI, hip circumference (HCF), waist circumference (WCF), fat mass, body weight, neck circumference (NCF), and height. *p≤0.05, ** p≤0.01, *** p≤0.001 indicate significant correlation between human plasma AKG level and BMI, HCF, WCF, fat mass and body weight. Results are presented as mean ± SEM. In (A-D) and (G-H), ∗p≤0.05 by one-way ANOVA followed by post hoc Turkey’s tests.

    Journal: bioRxiv

    Article Title: α-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation

    doi: 10.1101/796037

    Figure Lengend Snippet: Physiological relevance of AKG (A-D). The serum concentration of succinic acid (A), malic (B), hypoxanthine (C) and xanthine (D) in mice. Chow fed male C57BL/6 mice (10 weeks) were divided into three groups receiving non-exercise, endurance exercise (treadmill, 10 meters/minute, increased by 2 meters/minute every two minutes to exhaustion) or resistance exercise (ladder climbing for 40 min) (n = 8 per group). (E). Two tailed Pearson’s correlation coefficient analysis of plasma AKG level and the running distance of wheels. Chow fed male C57BL/6 mice (10 weeks) received one-day free access to running wheel. (n = 10 per group). (F). Two tailed Pearson’s correlation coefficient analysis of plasma AKG level and the ladder climbing time. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise (ladder climbing for 40 min). (n = 10 per group). (F). Serum AKG level after electric stimulation. Electric stimulation was performed in unilateral gastrocnemius for 40 min (1 ms width/50 Hz, 10 times, each time for 4 min, resting for 2 min between stimulates) in vivo in 10 weeks male C57BL/6 mice fed with chow diet (n = 8-9 per group). (G). Serum lactate concentration. Chow fed male C57BL/6 mice (10 weeks) received resistance exercise for 40 min. The serum lactate concentration was test before and immediately after exercise. (n = 10 per group). (H). Two tailed Pearson’s correlation coefficient analysis of human plasma AKG level and BMI, hip circumference (HCF), waist circumference (WCF), fat mass, body weight, neck circumference (NCF), and height. *p≤0.05, ** p≤0.01, *** p≤0.001 indicate significant correlation between human plasma AKG level and BMI, HCF, WCF, fat mass and body weight. Results are presented as mean ± SEM. In (A-D) and (G-H), ∗p≤0.05 by one-way ANOVA followed by post hoc Turkey’s tests.

    Article Snippet: Serum and tissue extracts were analyzed using LC–MS/MS analysis (Uplc1290-6470A QQQ Liquid chromatography-mass spectrometry instrument, Agilent technologies).

    Techniques: Concentration Assay, Mouse Assay, Two Tailed Test, In Vivo

    Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P

    Journal: Pharmacognosy Magazine

    Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats

    doi: 10.4103/pm.pm_340_17

    Figure Lengend Snippet: Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P

    Article Snippet: Equipments All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly.

    Techniques: Liquid Chromatography, Mass Spectrometry, Concentration Assay, Standard Deviation

    Estimated power versus putative type 1 error rate (α) with validation (Progenesis PPA measurements). a , power results for Progenesis PPA measurement data (as in ). At α = 0.05, the estimated power of ProPCA is 0.60, the estimated

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Increased Power for the Analysis of Label-free LC-MS/MS Proteomics Data by Combining Spectral Counts and Peptide Peak Attributes *

    doi: 10.1074/mcp.M110.002774

    Figure Lengend Snippet: Estimated power versus putative type 1 error rate (α) with validation (Progenesis PPA measurements). a , power results for Progenesis PPA measurement data (as in ). At α = 0.05, the estimated power of ProPCA is 0.60, the estimated

    Article Snippet: The msInspect/AMT peak alignment algorithm has been described ( , , ); the Progenesis LC-MS software utilizes a proprietary alignment algorithm.

    Techniques:

    Spike-in experiment to evaluate analysis of LC-MS data generated by a label-free LC-MS method . The experiment design involves two groups of samples: (i) serum samples with spike-in peptides, and (ii) serum samples alone. LC-MS data were generated by UPLC-QTOF mass spectrometer. Four software tools (msInspect, MZmine 2, Progenesis LC-MS, and XCMS) were applied for LC-MS data analysis to evaluate workflows implemented.

    Journal: Proteome Science

    Article Title: Using a spike-in experiment to evaluate analysis of LC-MS data

    doi: 10.1186/1477-5956-10-13

    Figure Lengend Snippet: Spike-in experiment to evaluate analysis of LC-MS data generated by a label-free LC-MS method . The experiment design involves two groups of samples: (i) serum samples with spike-in peptides, and (ii) serum samples alone. LC-MS data were generated by UPLC-QTOF mass spectrometer. Four software tools (msInspect, MZmine 2, Progenesis LC-MS, and XCMS) were applied for LC-MS data analysis to evaluate workflows implemented.

    Article Snippet: In Progenesis LC-MS, the landmark vector can be generated either automatically by the software for all LC-MS runs based on detected paired features (details are not provided by Progenesis) or can be selected manually by looking at each LC-MS map.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Generated, Mass Spectrometry, Software

    Representative chromatograms of RR extract analyzed on LCMS-IT-TOF. (A) UV chromatogram at 210 nm; (B) Total negative iron chromatogram; (C) Total positive iron chromatogram.

    Journal: Frontiers in Physiology

    Article Title: Radix Rehmanniae Extract Ameliorates Experimental Autoimmune Encephalomyelitis by Suppressing Macrophage-Derived Nitrative Damage

    doi: 10.3389/fphys.2018.00864

    Figure Lengend Snippet: Representative chromatograms of RR extract analyzed on LCMS-IT-TOF. (A) UV chromatogram at 210 nm; (B) Total negative iron chromatogram; (C) Total positive iron chromatogram.

    Article Snippet: We established two reliable qualitative and quantitative methods for quality control of RR by using LCMS-IT-TOF or HPLC-DAD systems.

    Techniques: Liquid Chromatography with Mass Spectroscopy

    Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu LCMS-8050 triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Efficient targeted tumor imaging and secreted endostatin gene delivery by anti-CD105 immunoliposomes

    doi: 10.1186/s13046-018-0712-8

    Figure Lengend Snippet: Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu LCMS-8050 triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP

    Article Snippet: Chromatographic separations were carried out using a Shimadzu LCMS-8050 triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system.

    Techniques: Mass Spectrometry, Concentration Assay, Serial Dilution, Liquid Chromatography with Mass Spectroscopy, In Vitro, In Vivo, Fluorescence, Derivative Assay, Flow Cytometry, Cytometry, Negative Control, Binding Assay, Transfection