Journal: bioRxiv

Article Title: A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease

doi: 10.1101/2023.08.30.555522

Figure Lengend Snippet: (A-D) Alveolar type 1 cell (AT1) fate differentiation of fdAT2 organoids in 10% human serum-containing medium on 2D culture. Experimental scheme of AT1 differentiation (A), a bright-field image of AT2 organoids upon AT1 differentiation (B), qRT-PCR analysis of AT1 markers AQP5 , CAV1 , and AGER , and an AT2 marker, SFTPC (C), and immunofluorescence imaging with CAV1, E-cadherin, β-actin, and DAPI (nuclei) (D). For RT-qPCT, data were normalised to fdAT2 organoids; mean ± SD, n = 3 independent repeats (*p<0.05, **p<0.01; unpaired t-test (two-tailed)). (E-G) AT1 differentiation of SFTPC -GFP-expressing fdAT2 organoids in a culture medium containing YAP signalling agonist, 10 μM LATS-IN-1, in the absence of Wnt agonists, in 3D organoid culture. Experimental scheme of AT1 differentiation (E), qRT-PCR analysis of AT1 markers AQP5 , CAV1 , and AGER , and an AT2 marker, SFTPC (F), and immunofluorescence imaging with CAV1, AGER, and DAPI (nuclei) (G). For RT-qPCR, data were normalised to fdAT2 organoids; mean ± SD, n=3 independent repeats (*p<0.05, **p<0.01; unpaired t-test (two-tailed)). (H) Explant culture of mouse precision-cut lung slices (PCLS) upon injecting SFTPC -GFP; EF1a -RFP human fdAT2 organoids. Organoids were dissociated and 2 x 10 5 single cells mixed with 100 μl of 50% matrigel and directly injected into each lobe of the lungs. PCLS of 150 μm thickness were cultured for 1 week in 10% fetal bovine serum-containing medium. Three independent lines of fdAT2 organoids were used for the explant culture (16402, 16587, 16392). (I-K) Immunofluorescence images of PCLS showing engrafted and AT1 differentiated human cells. RFP+ cells were monitored by a combination of cell type marker antibodies against SFTPC (I,J), and CAV1 (I) or AGER (J). DAPI, nuclei. Scale bars, 50 μm. Quantitation (K) of AT1-lineage positive human cells in the explants, by measuring the proportion of AT2 (SFTPC) and AT1 markers (CAV1 and AGER) co-localising with RFP (n = 53, SFTPC/CAV1 cells; n = 56, SFTPC/AGER cells; 6 lung slices from 3 biological replicates).

Article Snippet: For in vitro AT1 differentiation, AT2 organoids were dissociated into single cells and 1 x 10 cells were replated on matrigel-coated 12-well dishes and cultured either 1) in an AT1-promoting medium containing 10% human serum for 2 weeks or 2) in medium containing the LATS1 and 2 inhibitor LATS-IN-1 (10 mM, Cambridge Bioscience, CAY36623) and in the absence of CHIR99021 for a week.

Techniques: Quantitative RT-PCR, Marker, Immunofluorescence, Imaging, Two Tailed Test, Expressing, Injection, Cell Culture, Quantitation Assay

Journal: Preventing Chronic Disease

Article Title: Differences in Treating Tobacco Use Across National, State, and Public Hospital System Surveys

doi: 10.5888/pcd15.170575

Figure Lengend Snippet: Demographic Characteristics and Smoking Behavior Reported by Participants in Three Tobacco-Related Surveys, Louisiana, 2010 and 2013 a

Article Snippet: The percentage of 2013 LATS white respondents increased to 57% from 52% in 2010, while the percentage of 2013 TCI white participants decreased from 52% to 48%.

Techniques:

Journal: Preventing Chronic Disease

Article Title: Differences in Treating Tobacco Use Across National, State, and Public Hospital System Surveys

doi: 10.5888/pcd15.170575

Figure Lengend Snippet: Smoking Cessation Assistance Using 5 A’s (Ask, Advise, Assess, Assist, Arrange) Tobacco Cessation Intervention Reported by Respondents to 3 Surveys, Louisiana, 2010 and 2013 a

Article Snippet: The percentage of 2013 LATS white respondents increased to 57% from 52% in 2010, while the percentage of 2013 TCI white participants decreased from 52% to 48%.

Techniques:

Journal: Preventing Chronic Disease

Article Title: Differences in Treating Tobacco Use Across National, State, and Public Hospital System Surveys

doi: 10.5888/pcd15.170575

Figure Lengend Snippet: Adjusted a Odds Ratios of Having Received Any Component of the 5 A's Tobacco Cessation Intervention and a Quit Attempt, Respondents to 3 Surveys, Louisiana, 2010 and 2013

Article Snippet: The percentage of 2013 LATS white respondents increased to 57% from 52% in 2010, while the percentage of 2013 TCI white participants decreased from 52% to 48%.

Techniques:

Journal: Cell Proliferation

Article Title: AMOT 130 linking F‐actin to YAP is involved in intervertebral disc degeneration

doi: 10.1111/cpr.12492

Figure Lengend Snippet: Sequences of lentivirus Sh‐RNA

Article Snippet: Lentivirus vectors contain the LATS1 and LATS2 knockdown (Sh‐LATS A and Sh‐LATS B) or a negative control sequence (Sh‐Control) (GeneChem, Shanghai, China).

Techniques: Sequencing, Control