Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Impact of adropin on superoxide dismutase expression in pancreatic β‐cells of normoglycemic and diabetic rats. (A) Immunofluorescence labeling with anti‐superoxide dismutase antibody and anti‐insulin antibodies showed expression of superoxide dismutase in pancreatic β‐cells. (B) Quantification of the histological analysis showed a significant (*** p < 0.001) decrease in superoxide dismutase distribution in pancreatic endocrine cells and a significant (**** p < 0.0001) decrease in its localization in β‐cells of rats with diabetes when compared to the normal group. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Labeling

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on superoxide dismutase expression in pancreatic α‐cells of normal and diabetic rats. (A) Immunofluorescence staining using anti‐superoxide dismutase and anti‐glucagon antibodies showed expression of superoxide dismutase in pancreatic α‐cells. (B) Quantification of the histological analysis showed a significant (** p < 0.01) decrease in superoxide dismutase distribution in the pancreatic endocrine cells of diabetic rats compared to the normal group. α‐Cell expression of superoxide dismutase did not change with adropin treatment among all groups. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on catalase, superoxide dismutase and total glutathione activities in the serum samples of healthy and diabetic rats. (A) Catalase activity was significantly decreased in diabetic rats compared to normal controls. Adropin slightly increased catalase in DMT group. (B) Superoxide dismutase was slightly increased in DMT compared to the diabetic treated with adropin. (C) Total glutathione was significantly raised with adropin treatment in DMT compared to DMUT. n = 4–6. Data analysis was done using the ANOVA test. * p < 0.05.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Activity Assay

Journal: Biosensors

Article Title: Rapid and Simple Buffer Exchange Using Cation-Exchange Chromatography to Improve Point-of-Care Detection of Pharmacological Agents.

doi: 10.3390/bios13060635

Figure Lengend Snippet: Figure 3. Comparison of LFA outputs versus albuterol concentration (ppb) for selected urine simu- lants. Aggregated LFA readout values from all urine simulants (n = 9) (A), from concentrated urine simulant (B), high salt only urine simulant, (C) and dilute urine simulant (D).

Article Snippet: LFAs to detect albuterol (salbutamol) were acquired from ELabScience (Houston, TX, USA).

Techniques: Comparison, Concentration Assay

Journal: Biosensors

Article Title: Rapid and Simple Buffer Exchange Using Cation-Exchange Chromatography to Improve Point-of-Care Detection of Pharmacological Agents.

doi: 10.3390/bios13060635

Figure Lengend Snippet: Figure 4. Comparison of LFA readout from two human saliva samples. LFAs with no processing return the same positive result if the samples contain 20 ppb albuterol (A) and 0 ppb albuterol (B). In comparison, processing using the method in this paper results in a clear signal when 20 ppb of albuterol is added to the sample (C) compared to when no albuterol is added (D) using either visual inspection (left) or an optical reader (right).

Article Snippet: LFAs to detect albuterol (salbutamol) were acquired from ELabScience (Houston, TX, USA).

Techniques: Comparison

Journal: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Article Title: Profile of toll-like receptors on peripheral blood cells in relation to acute graft-versus-host disease after allogeneic stem cell transplantation.

doi: 10.1016/j.bbmt.2012.09.013

Figure Lengend Snippet: Figure 1. Expression of TLR-1, -5, and -9 on T lymphocytes and monocytes. Histogram plots of TLR-1, -5, and -9 expression in a patient with aGVHD and in a patient without aGVHD. The expression of surface (TLR-1 and -5) and intracellular (TLR-9) TLRs is reported with the corresponding negative control (mouse IgG1-PE). MFI is shown for each histogram. T lymphocytes of the patient with aGVHD have higher MFI values of TLR-5 and lower MFI values of TLR-1 and -9 compared with the patient without aGVHD (A). An analogous TLR profile is displayed by monocytes (B).

Article Snippet: Intracellular staining for TLR-3, -7, -8, and -9 was performed using an Imgenex Intracellular Staining Flow Assay Kit according to the manufacturer’s instructions.

Techniques: Expressing, Negative Control