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larry barcoding tool  (Addgene inc)
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Addgene inc larry barcoding tool
a , Experimental design for studying HSC heterogeneity with the Lineage and RNA RecoverY <t>(LARRY)</t> lentiviral <t>barcoding</t> library. All panels are representative from n = 3 independent labeling experiments (5 mice). b, Schemes of low-output (top) and high-output (bottom) HSC clones. c, Single cell map showing clonal HSC output activity values. Major cell populations are labeled. d, Distribution of high-output (output activity >1) and low-output (output activity <1) HSC cells and clones (shown as % of total HSCs). Mean ± S.D. e, Schemes of lineage balanced (top) and biased (bottom) HSC clones. f, Single cell map showing clonal Mk-bias values. g, Distribution of Mk-biased and Multilineage HSCs (cells and clones), Mk cells and non-Mk cells (shown as % of total). Mean ± S.D. h, Genes differentially expressed in low-output (right, n = 7254 cells) versus high-output (left, n = 3512 cells) HSCs. Genes with adjusted p-value<0.01 (Benjamini-Hochberg-corrected t-test) and fold-change>2 are colored. Selected genes are labeled. i, Genes differentially expressed in Mk-biased (right, n = 3399 cells) versus Multilineage (left, n = 3771 cells) HSCs. Genes with adjusted p-value<0.01 (Benjamini-Hochberg-corrected t-test) and fold-change>2 are colored. j , Single cell map of HSCs, colored by signature score values. k , Heatmap showing the Pearson correlation between different signature scores across all HSCs (n = 10837). l, Scatter plot of Mk-bias and output activity (log-transformed) for each HSC clone, colored by clone HSC frequency. Dotted lines are the output activity threshold ( A i = 1), and the Mk-bias threshold ( B i = 4). Only clones with HSC frequency > 0.005 are depicted (n = 62).
Larry Barcoding Tool, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/larry+barcoding+tool/pmc07579674-247-1-7?v=Addgene+inc
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larry barcoding tool - by Bioz Stars, 2026-06
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a , Experimental design for studying HSC heterogeneity with the Lineage and RNA RecoverY (LARRY) lentiviral barcoding library. All panels are representative from n = 3 independent labeling experiments (5 mice). b, Schemes of low-output (top) and high-output (bottom) HSC clones. c, Single cell map showing clonal HSC output activity values. Major cell populations are labeled. d, Distribution of high-output (output activity >1) and low-output (output activity <1) HSC cells and clones (shown as % of total HSCs). Mean ± S.D. e, Schemes of lineage balanced (top) and biased (bottom) HSC clones. f, Single cell map showing clonal Mk-bias values. g, Distribution of Mk-biased and Multilineage HSCs (cells and clones), Mk cells and non-Mk cells (shown as % of total). Mean ± S.D. h, Genes differentially expressed in low-output (right, n = 7254 cells) versus high-output (left, n = 3512 cells) HSCs. Genes with adjusted p-value<0.01 (Benjamini-Hochberg-corrected t-test) and fold-change>2 are colored. Selected genes are labeled. i, Genes differentially expressed in Mk-biased (right, n = 3399 cells) versus Multilineage (left, n = 3771 cells) HSCs. Genes with adjusted p-value<0.01 (Benjamini-Hochberg-corrected t-test) and fold-change>2 are colored. j , Single cell map of HSCs, colored by signature score values. k , Heatmap showing the Pearson correlation between different signature scores across all HSCs (n = 10837). l, Scatter plot of Mk-bias and output activity (log-transformed) for each HSC clone, colored by clone HSC frequency. Dotted lines are the output activity threshold ( A i = 1), and the Mk-bias threshold ( B i = 4). Only clones with HSC frequency > 0.005 are depicted (n = 62).

Journal: Nature

Article Title: Single-cell lineage tracing unveils a role for Tcf15 in haematopoiesis

doi: 10.1038/s41586-020-2503-6

Figure Lengend Snippet: a , Experimental design for studying HSC heterogeneity with the Lineage and RNA RecoverY (LARRY) lentiviral barcoding library. All panels are representative from n = 3 independent labeling experiments (5 mice). b, Schemes of low-output (top) and high-output (bottom) HSC clones. c, Single cell map showing clonal HSC output activity values. Major cell populations are labeled. d, Distribution of high-output (output activity >1) and low-output (output activity <1) HSC cells and clones (shown as % of total HSCs). Mean ± S.D. e, Schemes of lineage balanced (top) and biased (bottom) HSC clones. f, Single cell map showing clonal Mk-bias values. g, Distribution of Mk-biased and Multilineage HSCs (cells and clones), Mk cells and non-Mk cells (shown as % of total). Mean ± S.D. h, Genes differentially expressed in low-output (right, n = 7254 cells) versus high-output (left, n = 3512 cells) HSCs. Genes with adjusted p-value<0.01 (Benjamini-Hochberg-corrected t-test) and fold-change>2 are colored. Selected genes are labeled. i, Genes differentially expressed in Mk-biased (right, n = 3399 cells) versus Multilineage (left, n = 3771 cells) HSCs. Genes with adjusted p-value<0.01 (Benjamini-Hochberg-corrected t-test) and fold-change>2 are colored. j , Single cell map of HSCs, colored by signature score values. k , Heatmap showing the Pearson correlation between different signature scores across all HSCs (n = 10837). l, Scatter plot of Mk-bias and output activity (log-transformed) for each HSC clone, colored by clone HSC frequency. Dotted lines are the output activity threshold ( A i = 1), and the Mk-bias threshold ( B i = 4). Only clones with HSC frequency > 0.005 are depicted (n = 62).

Article Snippet: The LARRY barcoding tool is available at Addgene (#140024).

Techniques: Labeling, Clone Assay, Activity Assay, Transformation Assay