laminin antibody Search Results


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  • 94
    Thermo Fisher laminin 2 merosin
    Laminin 2 Merosin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laminin 2 merosin/product/Thermo Fisher
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    laminin 2 merosin - by Bioz Stars, 2020-08
    94/100 stars
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    94
    Millipore anti laminin antibody
    Uptake of ACPP is superimposed to loss of neuronal <t>laminin</t> in KA treated brain. (A) Laminin immunoreactivity in saline or KA-injected hippocampus 24 h after injection. (B) Progressive changes of laminin expression during the different phases of epileptogenesis. (C) Magnification of laminin loss in CA1–CA2 region 7 days after KA injection. The white arrow shows the limit between ACPP uptake and intact laminin (right panels), whereas no TAMRA fluorescence is detectable in the contralateral side and laminin is intact. TAMRA ACPPs (red), Laminin (white), Hoechst (blue). Scale bars: (A , B) 500 μm, (C) 200 μm.
    Anti Laminin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti laminin antibody/product/Millipore
    Average 94 stars, based on 4414 article reviews
    Price from $9.99 to $1999.99
    anti laminin antibody - by Bioz Stars, 2020-08
    94/100 stars
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    92
    Novocastra laminin
    Diaphragm formation during mouse development. ( A ) Sagittal sections of E15.5 wt, Igf2 +/- , Myod -/- and DM embryos were immunostained for Pax7 (α-Pax7, green) and <t>laminin</t> (α-Laminin, red). Myod -/- and DM diaphragms are thinner than those
    Laminin, supplied by Novocastra, used in various techniques. Bioz Stars score: 92/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laminin/product/Novocastra
    Average 92 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    laminin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    85
    Novocastra ncl merosin
    Diaphragm formation during mouse development. ( A ) Sagittal sections of E15.5 wt, Igf2 +/- , Myod -/- and DM embryos were immunostained for Pax7 (α-Pax7, green) and <t>laminin</t> (α-Laminin, red). Myod -/- and DM diaphragms are thinner than those
    Ncl Merosin, supplied by Novocastra, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncl merosin/product/Novocastra
    Average 85 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ncl merosin - by Bioz Stars, 2020-08
    85/100 stars
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    Image Search Results


    Uptake of ACPP is superimposed to loss of neuronal laminin in KA treated brain. (A) Laminin immunoreactivity in saline or KA-injected hippocampus 24 h after injection. (B) Progressive changes of laminin expression during the different phases of epileptogenesis. (C) Magnification of laminin loss in CA1–CA2 region 7 days after KA injection. The white arrow shows the limit between ACPP uptake and intact laminin (right panels), whereas no TAMRA fluorescence is detectable in the contralateral side and laminin is intact. TAMRA ACPPs (red), Laminin (white), Hoechst (blue). Scale bars: (A , B) 500 μm, (C) 200 μm.

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Gelatinase Biosensor Reports Cellular Remodeling During Epileptogenesis

    doi: 10.3389/fnsyn.2020.00015

    Figure Lengend Snippet: Uptake of ACPP is superimposed to loss of neuronal laminin in KA treated brain. (A) Laminin immunoreactivity in saline or KA-injected hippocampus 24 h after injection. (B) Progressive changes of laminin expression during the different phases of epileptogenesis. (C) Magnification of laminin loss in CA1–CA2 region 7 days after KA injection. The white arrow shows the limit between ACPP uptake and intact laminin (right panels), whereas no TAMRA fluorescence is detectable in the contralateral side and laminin is intact. TAMRA ACPPs (red), Laminin (white), Hoechst (blue). Scale bars: (A , B) 500 μm, (C) 200 μm.

    Article Snippet: Free-floating sections were rinsed three times in PBS and incubated for 20 min in PBS/0.2% Triton X-100 for permeabilization, and blocked in PBS/3% BSA for 1 h. Slices were then incubated in PBS/1% BSA/ 0.1% Triton X-100 overnight at 4°C with different primary antibodies: chicken anti-GFAP (1:300 dilution, ab4674; Abcam), rabbit anti-Iba1 (1:1,000, #019-19741; Wako), mouse anti-NeuN (1:300, MAB377; Millipore) and rabbit anti-laminin (1:300, L9393; Sigma–Adrich).

    Techniques: Injection, Expressing, Fluorescence

    Urethra decellularization. Immunofluorescence staining of ( a , c , e , g , I , k and m ) native urethra and ( b , d , f , h , j , l and n ) bioscaffolds generated using a 0.5% SDS solution ( * skeletal and ⦁ smooth muscle layers): collagens I to IV ( a–h ), elastin ( i–j ), fibronectin ( k–l ) and laminin ( m,n ) were tested. Images are from 5 μm thick sections. Scale bar: 100 μm.

    Journal: Scientific Reports

    Article Title: Acellular Urethra Bioscaffold: Decellularization of Whole Urethras for Tissue Engineering Applications

    doi: 10.1038/srep41934

    Figure Lengend Snippet: Urethra decellularization. Immunofluorescence staining of ( a , c , e , g , I , k and m ) native urethra and ( b , d , f , h , j , l and n ) bioscaffolds generated using a 0.5% SDS solution ( * skeletal and ⦁ smooth muscle layers): collagens I to IV ( a–h ), elastin ( i–j ), fibronectin ( k–l ) and laminin ( m,n ) were tested. Images are from 5 μm thick sections. Scale bar: 100 μm.

    Article Snippet: The antibodies were anti-collagen I-IV (1:50, Southern Biotech), fibronectin (1:200, Santa Cruz Biotech.), elastin (1:50, Santa Cruz Biotech.) and laminin (1:200, Sigma® ) and secondary Alexa 594-conjugated goat anti-rabbit and donkey anti-goat (1:200, InvitrogenTM ).

    Techniques: Immunofluorescence, Staining, Generated

    NGF administration induces growth of axons and migration of glial cells. ( A , B ) S-100 staining marking glial cells in NGF-treated ( A ) and control ( B ) pulps. ( C , D ) Neurofilament (NFP) staining marking axons in NGF-treated ( C ) and control ( D ) pulps. ( E , F ) Laminin-α2 (LN2) expression in NGF-treated ( E ) and control ( F ) pulps. Abbreviations: nf, nerve fibres; o, odontoblasts.

    Journal: Scientific Reports

    Article Title: Nerve growth factor signalling in pathology and regeneration of human teeth

    doi: 10.1038/s41598-017-01455-3

    Figure Lengend Snippet: NGF administration induces growth of axons and migration of glial cells. ( A , B ) S-100 staining marking glial cells in NGF-treated ( A ) and control ( B ) pulps. ( C , D ) Neurofilament (NFP) staining marking axons in NGF-treated ( C ) and control ( D ) pulps. ( E , F ) Laminin-α2 (LN2) expression in NGF-treated ( E ) and control ( F ) pulps. Abbreviations: nf, nerve fibres; o, odontoblasts.

    Article Snippet: Mouse anti-neurofilament proteins (NFP; Zymed labs, San Francisco, CA, USA) and anti-human laminin-α2 (merosin) (Chemicon International, Temecula, CA, USA) were diluted 1:50 and 1:2500, respectively.

    Techniques: Migration, Staining, Expressing

    Src induces formation of invadopodia-like protrusions in the intestine of wild type zebrafish larvae. (A–D) Sagittal confocal scans through the intestine of 74 hpf wild type and mlt larvae that express Src-mCherry (red) and Lifeact-GFP (green) in the intestinal epithelium. (A) In WT, Src (red) is localized at the apical (ap) and lateral epithelial cell membrane. (B) In mlt , Src also localizes to sites of actin-rich (green) invadopodia-like protrusions (arrowheads B) arising from the basal epithelial cell membrane (ba). (C) Constitutively active Src (caSrc; red) localizes to invadopodia-like protrusions (green) in mlt (arrowheads). (D) caSrc induces formation of the protrusions in WT (arrowheads). (E) Histological cross-sections through the intestine of a 74 hpf wild type larva showing caSrc-rich protrusions (green) protruding through small degraded regions of the basal lamina (laminin immunostain, red). Additional examples are shown in high power images (E, E′, and E″).

    Journal: PLoS Biology

    Article Title: Smooth Muscle Tension Induces Invasive Remodeling of the Zebrafish Intestine

    doi: 10.1371/journal.pbio.1001386

    Figure Lengend Snippet: Src induces formation of invadopodia-like protrusions in the intestine of wild type zebrafish larvae. (A–D) Sagittal confocal scans through the intestine of 74 hpf wild type and mlt larvae that express Src-mCherry (red) and Lifeact-GFP (green) in the intestinal epithelium. (A) In WT, Src (red) is localized at the apical (ap) and lateral epithelial cell membrane. (B) In mlt , Src also localizes to sites of actin-rich (green) invadopodia-like protrusions (arrowheads B) arising from the basal epithelial cell membrane (ba). (C) Constitutively active Src (caSrc; red) localizes to invadopodia-like protrusions (green) in mlt (arrowheads). (D) caSrc induces formation of the protrusions in WT (arrowheads). (E) Histological cross-sections through the intestine of a 74 hpf wild type larva showing caSrc-rich protrusions (green) protruding through small degraded regions of the basal lamina (laminin immunostain, red). Additional examples are shown in high power images (E, E′, and E″).

    Article Snippet: The laminin antibody (Sigma #L-9393) was used at 1∶50 or 1∶200 dilution; the cytokeratin antibody (Thermo Scientific clone AE1/AE3, MS-343-PO) was used at 1∶100 dilution.

    Techniques:

    Intestinal epithelial invasion in mlt larvae. (A–C) Live images of wild type (WT) and mlt larvae. In WT (A) the posterior intestine forms a smooth cylindrical tube (box), whereas in mlt at 74 hpf the intestinal contour is irregular (B). Cystic expansion of the intestine is evident in 86 hpf mlt larvae (C). (D–H) Histological cross-sections through the posterior intestine of larvae immunostained for laminin (green) and cytokeratin (red). The WT intestine is comprised of a simple epithelial sheet consisting of a single layer of cells, whereas in mlt epithelial stratification (asterisks) and invasive cells that have breached the basement membrane are evident (E–G arrowheads). The initial invasive behavior is followed by expansive growth and loss of epithelial architecture (H).

    Journal: PLoS Biology

    Article Title: Smooth Muscle Tension Induces Invasive Remodeling of the Zebrafish Intestine

    doi: 10.1371/journal.pbio.1001386

    Figure Lengend Snippet: Intestinal epithelial invasion in mlt larvae. (A–C) Live images of wild type (WT) and mlt larvae. In WT (A) the posterior intestine forms a smooth cylindrical tube (box), whereas in mlt at 74 hpf the intestinal contour is irregular (B). Cystic expansion of the intestine is evident in 86 hpf mlt larvae (C). (D–H) Histological cross-sections through the posterior intestine of larvae immunostained for laminin (green) and cytokeratin (red). The WT intestine is comprised of a simple epithelial sheet consisting of a single layer of cells, whereas in mlt epithelial stratification (asterisks) and invasive cells that have breached the basement membrane are evident (E–G arrowheads). The initial invasive behavior is followed by expansive growth and loss of epithelial architecture (H).

    Article Snippet: The laminin antibody (Sigma #L-9393) was used at 1∶50 or 1∶200 dilution; the cytokeratin antibody (Thermo Scientific clone AE1/AE3, MS-343-PO) was used at 1∶100 dilution.

    Techniques:

    Src inhibition rescues invasion but not formation of invadopodia-like protrusions in mlt . (A–C) Lateral views of live 5 dpf WT (A) and mlt (B, C) larvae. Treatment with the Src-I1 inhibitor rescues invasion in mlt (C). The size of the intestinal epithelium in the treated mlt larva (C) is reduced compared with the untreated mlt larva (B). (D–G) Histological cross-sections of 4 dpf WT (D) and mlt (E–G) Tg(miR194:Lifeact-GFP) larvae immunostained with antibodies against laminin (red) and GFP (green). Nuclei stained blue with DAPI. Arrows point to invadopodia-like protrusions arising from the basal epithelial cell membrane of Src-I1 treated mlt larvae (F, G). Pronounced invasion with distortion of intestinal architecture is evident in the untreated mlt larva (E). Note that invasion is markedly reduced in the Src-I1 treated mlt larvae despite the presence of the invadopodia-like protrusions (white arrows in F, G).

    Journal: PLoS Biology

    Article Title: Smooth Muscle Tension Induces Invasive Remodeling of the Zebrafish Intestine

    doi: 10.1371/journal.pbio.1001386

    Figure Lengend Snippet: Src inhibition rescues invasion but not formation of invadopodia-like protrusions in mlt . (A–C) Lateral views of live 5 dpf WT (A) and mlt (B, C) larvae. Treatment with the Src-I1 inhibitor rescues invasion in mlt (C). The size of the intestinal epithelium in the treated mlt larva (C) is reduced compared with the untreated mlt larva (B). (D–G) Histological cross-sections of 4 dpf WT (D) and mlt (E–G) Tg(miR194:Lifeact-GFP) larvae immunostained with antibodies against laminin (red) and GFP (green). Nuclei stained blue with DAPI. Arrows point to invadopodia-like protrusions arising from the basal epithelial cell membrane of Src-I1 treated mlt larvae (F, G). Pronounced invasion with distortion of intestinal architecture is evident in the untreated mlt larva (E). Note that invasion is markedly reduced in the Src-I1 treated mlt larvae despite the presence of the invadopodia-like protrusions (white arrows in F, G).

    Article Snippet: The laminin antibody (Sigma #L-9393) was used at 1∶50 or 1∶200 dilution; the cytokeratin antibody (Thermo Scientific clone AE1/AE3, MS-343-PO) was used at 1∶100 dilution.

    Techniques: Inhibition, Staining

    Activation of oncogenic signaling enhances sensitivity of mlt heterozygotes to oxidative stress. (A, B) Lateral views of live, menadione treated 5 dpf axin mutant larvae that express mutant KRAS in the intestinal epithelium ( Kras-axin ). (A) Hypertrophy of the intestinal epithelium in a Kras-axin larva that is homozygous for the wildtype myh11 allele (mlt +/+) larvae is unchanged by treatment with menadione. (B) Menadione treatment causes pronounced cystic expansion of the posterior intestinal epithelium of the Kras-axin mlt heterozygote (arrowheads) that resembles the homozygous mlt phenotype. Inset, immunolabeling of the basal lamina (laminin, green) and epithelium (cytokeratin, red) shows epithelial cell invasion through the basement membrane. (C–E) Histological cross-sections through the intestine of immunostained larvae show invasive cells in menadione treated Kras-axin mlt heterozygotes (arrowheads; D, E). Invasion is not detected in menadione-treated Kras-axin mlt larvae that are homozygous for the wildtype myh11 allele (C).

    Journal: PLoS Biology

    Article Title: Smooth Muscle Tension Induces Invasive Remodeling of the Zebrafish Intestine

    doi: 10.1371/journal.pbio.1001386

    Figure Lengend Snippet: Activation of oncogenic signaling enhances sensitivity of mlt heterozygotes to oxidative stress. (A, B) Lateral views of live, menadione treated 5 dpf axin mutant larvae that express mutant KRAS in the intestinal epithelium ( Kras-axin ). (A) Hypertrophy of the intestinal epithelium in a Kras-axin larva that is homozygous for the wildtype myh11 allele (mlt +/+) larvae is unchanged by treatment with menadione. (B) Menadione treatment causes pronounced cystic expansion of the posterior intestinal epithelium of the Kras-axin mlt heterozygote (arrowheads) that resembles the homozygous mlt phenotype. Inset, immunolabeling of the basal lamina (laminin, green) and epithelium (cytokeratin, red) shows epithelial cell invasion through the basement membrane. (C–E) Histological cross-sections through the intestine of immunostained larvae show invasive cells in menadione treated Kras-axin mlt heterozygotes (arrowheads; D, E). Invasion is not detected in menadione-treated Kras-axin mlt larvae that are homozygous for the wildtype myh11 allele (C).

    Article Snippet: The laminin antibody (Sigma #L-9393) was used at 1∶50 or 1∶200 dilution; the cytokeratin antibody (Thermo Scientific clone AE1/AE3, MS-343-PO) was used at 1∶100 dilution.

    Techniques: Activation Assay, Mutagenesis, Immunolabeling

    Smooth muscle contraction drives epithelial invasion but does not alter tissue rigidity. (A–C) Lateral views of live 5 dpf larvae injected with Sma or control morpholino. (A) Sma knockdown has no effect on WT intestinal morphology. (B) Control morpholino injection in mlt . (C) Sma knockdown rescues invasion in mlt . Residual invasive cells persist in this Sma deficient mlt larva (arrowhead). (D–I) Histological cross-sections through the posterior intestine of 74 hpf WT and mlt larvae immunostained with anti-keratin (red) and anti-laminin (green) antibodies. (D) WT. (E, F) mlt larvae injected with control (E) and Sma (D, F) morpholinos. Invasive cells in mlt (arrowheads, E) are rescued by Sma knockdown. (G, H) Injection of a morpholino targeting the high molecular weight isoform of Caldesmon (cald) has no effect on WT intestinal morphology but induces invasion (arrowhead) and stratification (asterisk) in an mlt heterozygote. (I) Treatment of an mlt heterozygote with L-NAME causes invasion (arrowhead) and epithelial stratification (asterisk). (J) Force displacement measurements show identical compliance of intestines dissected from mlt and WT larvae before the phenotype develops at 70 hpf, and a modest increase in compliance at the outer surface of the intestine (

    Journal: PLoS Biology

    Article Title: Smooth Muscle Tension Induces Invasive Remodeling of the Zebrafish Intestine

    doi: 10.1371/journal.pbio.1001386

    Figure Lengend Snippet: Smooth muscle contraction drives epithelial invasion but does not alter tissue rigidity. (A–C) Lateral views of live 5 dpf larvae injected with Sma or control morpholino. (A) Sma knockdown has no effect on WT intestinal morphology. (B) Control morpholino injection in mlt . (C) Sma knockdown rescues invasion in mlt . Residual invasive cells persist in this Sma deficient mlt larva (arrowhead). (D–I) Histological cross-sections through the posterior intestine of 74 hpf WT and mlt larvae immunostained with anti-keratin (red) and anti-laminin (green) antibodies. (D) WT. (E, F) mlt larvae injected with control (E) and Sma (D, F) morpholinos. Invasive cells in mlt (arrowheads, E) are rescued by Sma knockdown. (G, H) Injection of a morpholino targeting the high molecular weight isoform of Caldesmon (cald) has no effect on WT intestinal morphology but induces invasion (arrowhead) and stratification (asterisk) in an mlt heterozygote. (I) Treatment of an mlt heterozygote with L-NAME causes invasion (arrowhead) and epithelial stratification (asterisk). (J) Force displacement measurements show identical compliance of intestines dissected from mlt and WT larvae before the phenotype develops at 70 hpf, and a modest increase in compliance at the outer surface of the intestine (

    Article Snippet: The laminin antibody (Sigma #L-9393) was used at 1∶50 or 1∶200 dilution; the cytokeratin antibody (Thermo Scientific clone AE1/AE3, MS-343-PO) was used at 1∶100 dilution.

    Techniques: Injection, Molecular Weight

    Oxidative stress induces invasive remodeling in mlt heterozygous larvae. (A–C) Lateral images of live WT (A), mlt homozygous (B), and mlt heterozygous larvae (C). The WT and mlt heterozygous larvae received 3 h of treatment with Menadione beginning at 73 hpf. Menadione treated heterozygote (C) larvae have an intestinal phenotype (arrowheads) resembling the untreated mlt homozygous larvae (B). (D–F) Corresponding histological cross-sections (representative of larvae in A, C) with intestinal epithelial cells labeled red (anti-keratin immunostain) and basement membrane in green (anti-laminin immunostain). Menadione causes epithelial cell invasion (arrows) and stratification (asterisks) in mlt heterozygous larvae (E, F) but does not affect epithelial architecture in the WT intestines (D). (G–I) Sagittal confocal scans through the intestine of WT and mlt heterozygotes treated with menadione. Both larvae express LifeAct-GFP in a subset of intestinal epithelial cells. Actin-rich invadopodia-like protrusions (green) are seen arising from the basal epithelial cell membrane of menadione treated heterozygous larvae (arrowheads, H, I). Actin is located nearly exclusively in the apical brush border of WT epithelial cells (G): Red -membrane mCherry; ba, basal epithelial cell border; ap, apical epithelial cell border.

    Journal: PLoS Biology

    Article Title: Smooth Muscle Tension Induces Invasive Remodeling of the Zebrafish Intestine

    doi: 10.1371/journal.pbio.1001386

    Figure Lengend Snippet: Oxidative stress induces invasive remodeling in mlt heterozygous larvae. (A–C) Lateral images of live WT (A), mlt homozygous (B), and mlt heterozygous larvae (C). The WT and mlt heterozygous larvae received 3 h of treatment with Menadione beginning at 73 hpf. Menadione treated heterozygote (C) larvae have an intestinal phenotype (arrowheads) resembling the untreated mlt homozygous larvae (B). (D–F) Corresponding histological cross-sections (representative of larvae in A, C) with intestinal epithelial cells labeled red (anti-keratin immunostain) and basement membrane in green (anti-laminin immunostain). Menadione causes epithelial cell invasion (arrows) and stratification (asterisks) in mlt heterozygous larvae (E, F) but does not affect epithelial architecture in the WT intestines (D). (G–I) Sagittal confocal scans through the intestine of WT and mlt heterozygotes treated with menadione. Both larvae express LifeAct-GFP in a subset of intestinal epithelial cells. Actin-rich invadopodia-like protrusions (green) are seen arising from the basal epithelial cell membrane of menadione treated heterozygous larvae (arrowheads, H, I). Actin is located nearly exclusively in the apical brush border of WT epithelial cells (G): Red -membrane mCherry; ba, basal epithelial cell border; ap, apical epithelial cell border.

    Article Snippet: The laminin antibody (Sigma #L-9393) was used at 1∶50 or 1∶200 dilution; the cytokeratin antibody (Thermo Scientific clone AE1/AE3, MS-343-PO) was used at 1∶100 dilution.

    Techniques: Labeling

    Actin-rich protrusions in invasive epithelial cells of the mlt intestine. (A, B) Full thickness 3-D rendering of sagittal confocal sections through the intestine of 78 hpf wild type (WT) (A) and mlt (B) larvae. Actin is labeled by transgenic Lifeact-GFP expression (green). (A) In WT, the majority of the label is present in the epithelial cell apical brush border (bracket). (B) In mlt , actin-rich invadopodia-like protrusions of the basal epithelial cell membrane are detected (arrows), in addition to brush border actin (bracket). (C) Time lapse analysis of protrusion development. Single sagittal confocal scans through the intestine of a mlt larva beginning at 74 hpf. Basal invadopodia-like protrusions (arrowheads) precede cell invasion, which is first detected at 135 min. Asterisks mark invasive cells at 270 min (see also B). (D–F) Histological cross-sections through the intestine of 74 hpf immunostained mlt larvae. Basement membrane is labeled red (laminin immunostain) and actin labeled green (GFP immunostain in Lifeact-GFP transgenics). Nuclei stained blue with DAPI. Actin rich protrusions in mlt co-localize with sites of basal lamina degradation (arrowheads and insets E, F). During progression of the phenotype epithelial cells invade the tissue stroma through degraded regions of the basal lamina (asterisks in F). ap, apical epithelial cell border; ba, basal cell epithelial cell border.

    Journal: PLoS Biology

    Article Title: Smooth Muscle Tension Induces Invasive Remodeling of the Zebrafish Intestine

    doi: 10.1371/journal.pbio.1001386

    Figure Lengend Snippet: Actin-rich protrusions in invasive epithelial cells of the mlt intestine. (A, B) Full thickness 3-D rendering of sagittal confocal sections through the intestine of 78 hpf wild type (WT) (A) and mlt (B) larvae. Actin is labeled by transgenic Lifeact-GFP expression (green). (A) In WT, the majority of the label is present in the epithelial cell apical brush border (bracket). (B) In mlt , actin-rich invadopodia-like protrusions of the basal epithelial cell membrane are detected (arrows), in addition to brush border actin (bracket). (C) Time lapse analysis of protrusion development. Single sagittal confocal scans through the intestine of a mlt larva beginning at 74 hpf. Basal invadopodia-like protrusions (arrowheads) precede cell invasion, which is first detected at 135 min. Asterisks mark invasive cells at 270 min (see also B). (D–F) Histological cross-sections through the intestine of 74 hpf immunostained mlt larvae. Basement membrane is labeled red (laminin immunostain) and actin labeled green (GFP immunostain in Lifeact-GFP transgenics). Nuclei stained blue with DAPI. Actin rich protrusions in mlt co-localize with sites of basal lamina degradation (arrowheads and insets E, F). During progression of the phenotype epithelial cells invade the tissue stroma through degraded regions of the basal lamina (asterisks in F). ap, apical epithelial cell border; ba, basal cell epithelial cell border.

    Article Snippet: The laminin antibody (Sigma #L-9393) was used at 1∶50 or 1∶200 dilution; the cytokeratin antibody (Thermo Scientific clone AE1/AE3, MS-343-PO) was used at 1∶100 dilution.

    Techniques: Labeling, Transgenic Assay, Expressing, Staining

    HRT have fewer oxidative muscle fibers and impaired exercise-induced angiogenesis. Plantaris muscles from sedentary (SED) and exercise-trained (EXT) rats were frozen in N 2 -cooled isopentane and cut into 6-µm cross-sections. A : Sections were stained with antibodies against laminin (white) and myosin heavy chain I (green) and visualized using fluorescent secondary antibodies under 100× magnification. Type I fiber content was expressed as % of total muscle fibers counted. B : Capillary density (capillaries/mm 2 ) was calculated in sections stained with an antibody against the endothelial marker CD31 (red). Nuclei were visualized with DAPI stain (blue). n = 4–5/group. * P

    Journal: Diabetes

    Article Title: Resistance to Aerobic Exercise Training Causes Metabolic Dysfunction and Reveals Novel Exercise-Regulated Signaling Networks

    doi: 10.2337/db13-0062

    Figure Lengend Snippet: HRT have fewer oxidative muscle fibers and impaired exercise-induced angiogenesis. Plantaris muscles from sedentary (SED) and exercise-trained (EXT) rats were frozen in N 2 -cooled isopentane and cut into 6-µm cross-sections. A : Sections were stained with antibodies against laminin (white) and myosin heavy chain I (green) and visualized using fluorescent secondary antibodies under 100× magnification. Type I fiber content was expressed as % of total muscle fibers counted. B : Capillary density (capillaries/mm 2 ) was calculated in sections stained with an antibody against the endothelial marker CD31 (red). Nuclei were visualized with DAPI stain (blue). n = 4–5/group. * P

    Article Snippet: Sections were stained with antibodies against laminin (Sigma-Aldrich) and either myosin heavy chain I (4.951; DHSB) or myosin heavy chain IIA (SC-71; DHSB), or the endothelial marker CD31 (Serotec MCA1334EL), and visualized using fluorescent secondary antibodies under 100× magnification.

    Techniques: Staining, Marker

    Histology of cerebral microvessels in control, CI and Aβ+CI groups. Microphotographs showed laminin-stained microvessels in the core of ipsilateral (right) striatum at week 1 (A–C) and week 4 (D–F) post insults. Quantitative analysis of the whole striatal core showed that average density of dilated microvessels (i.e. diameter greater than 10 µm) in CI and Aβ+CI groups was significantly higher at week 1 than those at week 4. As induced injury advanced, at week 4 regular vasculature was almost absent in the Aβ+CI group. Letter “C” indicates significant differences when compared to the control group, p

    Journal: PLoS ONE

    Article Title: Hemodynamic Effects of Combined Focal Cerebral Ischemia and Amyloid Protein Toxicity in a Rat Model: A Functional CT Study

    doi: 10.1371/journal.pone.0100575

    Figure Lengend Snippet: Histology of cerebral microvessels in control, CI and Aβ+CI groups. Microphotographs showed laminin-stained microvessels in the core of ipsilateral (right) striatum at week 1 (A–C) and week 4 (D–F) post insults. Quantitative analysis of the whole striatal core showed that average density of dilated microvessels (i.e. diameter greater than 10 µm) in CI and Aβ+CI groups was significantly higher at week 1 than those at week 4. As induced injury advanced, at week 4 regular vasculature was almost absent in the Aβ+CI group. Letter “C” indicates significant differences when compared to the control group, p

    Article Snippet: Sections were then stained with laminin primary antibody (1∶1000, rabbit anti-rat Laminin, Sigma-Aldrich).

    Techniques: Staining

    The RPE influences the ability of retinal ganglion cells to polarize in ectopic positions.  (a)  Head region of wild-type, morphant and mutant embryos, showing the distribution of pigment around the retina. Two examples of  nok  morpholino-injected embryos (0.17 pmoles per embryo) are shown.  (b)  Cryosection of the eye from a  nok -/ - ,  ath5:gap-gfp  transgenic embryo, labeled with an anti-laminin 1 antibody (red). The bright-field image, inverted and pseudo-colored in blue, shows the distribution of the retinal pigment epithelium (RPE). Arrowheads indicate  ath5:gap-gfp -positive retinal ganglion cells. D, dorsal; L, lateral; M, medial. Scale bar, 20 μm.  (c)  Transmission electron micrograph of the apical region of a  nok  mutant retina, showing the distribution of the RPE cells (dotted lines). Inset, a higher magnification of the boxed region, showing two apparent transverse-sectioned axons (Ax) at the RPE-free apical surface of the retina. Scale bars: low-magnification image, 5 μm; inset, 0.5 μm.  (d)  Transmission electron micrographs of the apical region of a wild-type (Wt) and a  has -/-  embryo. The RPE (blue arrows) is a very organized simple epithelium in the wild type, and it seems disrupted in the  has  mutant. Nevertheless, the picture does not show any actual gap between the RPE cells in the  has -/- embryo. Red arrowheads indicate mitotic cells in a normal (apical) position in the wild-type, and in an ectopic position in the mutant. Scale bar, 5 μm.  (e)  Fluorescence intensity profiles made on confocal sections of 48 hpf  ath5:gap-gfp  transgenic embryos labeled with an anti-RPE antibody (Zpr-2). For the measurements a 20-pixel wide line was drawn along a radius of the retina as in (f). The intensity profile of the green channel (GFP) was plotted and the values were normalized (maximum intensity) and averaged for each embryo; the resulting plots were then normalized to each other (integrated intensity). To compare the profiles in relation to the distribution of the RPE, we positioned the line either in regions where zpr-2 immunoreactivity was detected (RPE) or not (NO RPE). For the  nok  mutants, only measurements of areas without detectable RPE were used. Measurements were made in three to ten different areas from one wild-type, five  nok  mutant, four  nok  morphant and three  has  mutant retinas. The inset picture shows a region of a wild-type retina, like those used for the measurements, which is aligned with the profile plot to show the correspondence with the retinal layers. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; OFL, optic fiber layer; OPL, outer plexiform layer; PL, photoreceptor layer.  (f)  Optical sections of wild-type and mutant retinas of  ath5:gap-gfp  transgenic embryos labeled in red with the Zpr-2 antibody, which stains the RPE. The yellow rectangles show an example of the lines used for the measurements presented in (e). Scale bar, 25 μm.

    Journal: Neural Development

    Article Title: Polarization and orientation of retinal ganglion cells in vivo

    doi: 10.1186/1749-8104-1-2

    Figure Lengend Snippet: The RPE influences the ability of retinal ganglion cells to polarize in ectopic positions. (a) Head region of wild-type, morphant and mutant embryos, showing the distribution of pigment around the retina. Two examples of nok morpholino-injected embryos (0.17 pmoles per embryo) are shown. (b) Cryosection of the eye from a nok -/ - , ath5:gap-gfp transgenic embryo, labeled with an anti-laminin 1 antibody (red). The bright-field image, inverted and pseudo-colored in blue, shows the distribution of the retinal pigment epithelium (RPE). Arrowheads indicate ath5:gap-gfp -positive retinal ganglion cells. D, dorsal; L, lateral; M, medial. Scale bar, 20 μm. (c) Transmission electron micrograph of the apical region of a nok mutant retina, showing the distribution of the RPE cells (dotted lines). Inset, a higher magnification of the boxed region, showing two apparent transverse-sectioned axons (Ax) at the RPE-free apical surface of the retina. Scale bars: low-magnification image, 5 μm; inset, 0.5 μm. (d) Transmission electron micrographs of the apical region of a wild-type (Wt) and a has -/- embryo. The RPE (blue arrows) is a very organized simple epithelium in the wild type, and it seems disrupted in the has mutant. Nevertheless, the picture does not show any actual gap between the RPE cells in the has -/- embryo. Red arrowheads indicate mitotic cells in a normal (apical) position in the wild-type, and in an ectopic position in the mutant. Scale bar, 5 μm. (e) Fluorescence intensity profiles made on confocal sections of 48 hpf ath5:gap-gfp transgenic embryos labeled with an anti-RPE antibody (Zpr-2). For the measurements a 20-pixel wide line was drawn along a radius of the retina as in (f). The intensity profile of the green channel (GFP) was plotted and the values were normalized (maximum intensity) and averaged for each embryo; the resulting plots were then normalized to each other (integrated intensity). To compare the profiles in relation to the distribution of the RPE, we positioned the line either in regions where zpr-2 immunoreactivity was detected (RPE) or not (NO RPE). For the nok mutants, only measurements of areas without detectable RPE were used. Measurements were made in three to ten different areas from one wild-type, five nok mutant, four nok morphant and three has mutant retinas. The inset picture shows a region of a wild-type retina, like those used for the measurements, which is aligned with the profile plot to show the correspondence with the retinal layers. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; OFL, optic fiber layer; OPL, outer plexiform layer; PL, photoreceptor layer. (f) Optical sections of wild-type and mutant retinas of ath5:gap-gfp transgenic embryos labeled in red with the Zpr-2 antibody, which stains the RPE. The yellow rectangles show an example of the lines used for the measurements presented in (e). Scale bar, 25 μm.

    Article Snippet: The primary antibodies, diluted in the blocking solution, were as follows: Zn-5, 1/100 to 1/500 dilution (mAb anti-Ben/DM-GRASP, specific for RGCs in the differentiating neural retina; Zebrafish International Resource Center (ZIRC), Eugene, OR, USA; Zpr-2, 1/100 dilution (mAb specific for retinal pigment epithelium (RPE); ZIRC); anti-laminin 1, 1/60 dilution (poly-clonal antibody (pAb), L9393; Sigma, St Louis, MO, USA); anti-Tau 1, 1/500 dilution (pAb; Dr Itzhak Fischer); anti-aPKC-ζ, 1/250 to 1/500 dilution (pAb; New England Biolabs, Hitchin, UK); and anti-α-catenin, 1/2,000 dilution (pAb, Sigma).

    Techniques: Mutagenesis, Injection, Transgenic Assay, Labeling, Transmission Assay, Fluorescence

    Laminin α2 exposure attenuates radiation induced GSC cell death and increases DNA repair

    Journal: Annals of neurology

    Article Title: Laminin alpha 2 enables glioblastoma stem cell growth

    doi: 10.1002/ana.23674

    Figure Lengend Snippet: Laminin α2 exposure attenuates radiation induced GSC cell death and increases DNA repair

    Article Snippet: Tissue microarray analysis was performed as previously described [ ] using the laminin α2 antibody (Millipore).

    Techniques:

    Laminin α2 is required for GSC growth

    Journal: Annals of neurology

    Article Title: Laminin alpha 2 enables glioblastoma stem cell growth

    doi: 10.1002/ana.23674

    Figure Lengend Snippet: Laminin α2 is required for GSC growth

    Article Snippet: Tissue microarray analysis was performed as previously described [ ] using the laminin α2 antibody (Millipore).

    Techniques:

    Laminin α2 chain expression correlates with glioma patient survival, and is differentially expressed in GBM molecular subtypes

    Journal: Annals of neurology

    Article Title: Laminin alpha 2 enables glioblastoma stem cell growth

    doi: 10.1002/ana.23674

    Figure Lengend Snippet: Laminin α2 chain expression correlates with glioma patient survival, and is differentially expressed in GBM molecular subtypes

    Article Snippet: Tissue microarray analysis was performed as previously described [ ] using the laminin α2 antibody (Millipore).

    Techniques: Expressing

    Laminin α2 chain is elevated in GBM

    Journal: Annals of neurology

    Article Title: Laminin alpha 2 enables glioblastoma stem cell growth

    doi: 10.1002/ana.23674

    Figure Lengend Snippet: Laminin α2 chain is elevated in GBM

    Article Snippet: Tissue microarray analysis was performed as previously described [ ] using the laminin α2 antibody (Millipore).

    Techniques:

    Vascular and cellular response after non-transformed cell implantation in VOGIM A. PI staining (red) and GFP positive area to monitor cell death and tumor growth respectively in brain slices (top) in comparison to astrocytes cell implantation (bottom). Given are cell growth at day 4 and 8. Arrows indicate tumor bulk. Scale bar represents 500 μm. B. Quantification of bulk cell growth area of gliomas (F98) and primary astrocytes in brain slices. C. Vascularization in peritumoral area (TZ II). Vessels are stained for Laminin (blue). Glioma cells (F98, left) or astrocytes (right) are displayed in green and cell growth is indicated by the white dotted line. Arrows indicate intra-tumoral vessels in glioma-implanted brains. Scale bar represents 50 μm. D. Quantification of vessel length, junctions and branches in glioma implanted slices (green) and astrocytes-implanted brain slices (green). Images with 288 μm × 288 μm size were used for quantification. Statistical analysis was performed with Student's t -test (* P

    Journal: Oncotarget

    Article Title: A versatile ex vivo technique for assaying tumor angiogenesis and microglia in the brain

    doi:

    Figure Lengend Snippet: Vascular and cellular response after non-transformed cell implantation in VOGIM A. PI staining (red) and GFP positive area to monitor cell death and tumor growth respectively in brain slices (top) in comparison to astrocytes cell implantation (bottom). Given are cell growth at day 4 and 8. Arrows indicate tumor bulk. Scale bar represents 500 μm. B. Quantification of bulk cell growth area of gliomas (F98) and primary astrocytes in brain slices. C. Vascularization in peritumoral area (TZ II). Vessels are stained for Laminin (blue). Glioma cells (F98, left) or astrocytes (right) are displayed in green and cell growth is indicated by the white dotted line. Arrows indicate intra-tumoral vessels in glioma-implanted brains. Scale bar represents 50 μm. D. Quantification of vessel length, junctions and branches in glioma implanted slices (green) and astrocytes-implanted brain slices (green). Images with 288 μm × 288 μm size were used for quantification. Statistical analysis was performed with Student's t -test (* P

    Article Snippet: Primary antibodies laminin (Sigma-Aldrich) were diluted in a concentration of 1:1000 in 3% horse serum (HS) or fetal calf serum (FCS) and 0,2% Triton X-100 as the blocking solution and subsequently incubated for overnight at 4°C.

    Techniques: Transformation Assay, Staining

    Vascular and cellular response after temozolomide treatment in VOGIM A. Cell death monitoring (PI staining, white signal) in native slices under untreated control conditions (top) and after temozolomide (TMZ, 100 μM) treatment (bottom). Given is cell death at day 4 and day 8. Scale bar represents 500 μm. B. Quantification of cell death in brain slices after temozolomide (TMZ, 100 μM) treatment. Untreated controls are displayed in blue, TMZ treated group is given in red. C. Vascularization and microglial distribution of brain areas after temozolomide (TMZ) treatment. Vessels are stained for Laminin (blue) and microglial cells are displayed in green. Scale bar represents 20 μm. D. Quantification of the numbers of perivascular microglial cells after temozolomide (TMZ) treatment and in untreated (control) brain slices. E. Quantification of vessel length, junctions and branches in brain slices treated with temozolomide (TMZ) or untreated (control). Images with 288 μm × 288 μm size were used for quantification. Statistical analysis was performed with Student's t -test (* P

    Journal: Oncotarget

    Article Title: A versatile ex vivo technique for assaying tumor angiogenesis and microglia in the brain

    doi:

    Figure Lengend Snippet: Vascular and cellular response after temozolomide treatment in VOGIM A. Cell death monitoring (PI staining, white signal) in native slices under untreated control conditions (top) and after temozolomide (TMZ, 100 μM) treatment (bottom). Given is cell death at day 4 and day 8. Scale bar represents 500 μm. B. Quantification of cell death in brain slices after temozolomide (TMZ, 100 μM) treatment. Untreated controls are displayed in blue, TMZ treated group is given in red. C. Vascularization and microglial distribution of brain areas after temozolomide (TMZ) treatment. Vessels are stained for Laminin (blue) and microglial cells are displayed in green. Scale bar represents 20 μm. D. Quantification of the numbers of perivascular microglial cells after temozolomide (TMZ) treatment and in untreated (control) brain slices. E. Quantification of vessel length, junctions and branches in brain slices treated with temozolomide (TMZ) or untreated (control). Images with 288 μm × 288 μm size were used for quantification. Statistical analysis was performed with Student's t -test (* P

    Article Snippet: Primary antibodies laminin (Sigma-Aldrich) were diluted in a concentration of 1:1000 in 3% horse serum (HS) or fetal calf serum (FCS) and 0,2% Triton X-100 as the blocking solution and subsequently incubated for overnight at 4°C.

    Techniques: Staining

    Analysis the drug sensitivity of VOGIM method A. PI staining (red) and GFP positive area (green) monitors cell death and tumor growth respectively in brain slices treated with temozolamide (TMZ, 100 μM). Untreated brain slices served as control samples and analysis was performed on day 4 and 8. Scale bar displays 500 μm. B. Quantification of cell death after temozolamide (TMZ) treatment in comparison to untreated control samples on day 4 and 8. C. Quantification of tumor growth after temozolamide (TMZ) treatment in comparison to untreated controls at day 8. D. Peritumoral (TZ II) vascularization of TMZ treated brain slices (CX3CR1-GFP positive) and untreated controls. Laminin staining (blue) was utilized for vessel visualization. Microglial cells are displayed in green. Scale bar in top images represents 200 μm, scale bar in bottom images shows 20 μm. E. Quantification of the numbers of perivascular microglial cells in peritumoral area (TZ II) of temozolomide (TMZ) treated and untreated tumor-implanted brain slices. Images with 288 μm × 288 μm size were used for quantification. F. Distribution of vessel diameters (μm) in peritumoral areas after temozolomide (TMZ) treatment and in untreated controls. G. Quantification of vessel length, junctions and branches in glioma implanted untreated slices and temozolomide treated brain slices. Statistical analysis was performed with Student's t -test (* P

    Journal: Oncotarget

    Article Title: A versatile ex vivo technique for assaying tumor angiogenesis and microglia in the brain

    doi:

    Figure Lengend Snippet: Analysis the drug sensitivity of VOGIM method A. PI staining (red) and GFP positive area (green) monitors cell death and tumor growth respectively in brain slices treated with temozolamide (TMZ, 100 μM). Untreated brain slices served as control samples and analysis was performed on day 4 and 8. Scale bar displays 500 μm. B. Quantification of cell death after temozolamide (TMZ) treatment in comparison to untreated control samples on day 4 and 8. C. Quantification of tumor growth after temozolamide (TMZ) treatment in comparison to untreated controls at day 8. D. Peritumoral (TZ II) vascularization of TMZ treated brain slices (CX3CR1-GFP positive) and untreated controls. Laminin staining (blue) was utilized for vessel visualization. Microglial cells are displayed in green. Scale bar in top images represents 200 μm, scale bar in bottom images shows 20 μm. E. Quantification of the numbers of perivascular microglial cells in peritumoral area (TZ II) of temozolomide (TMZ) treated and untreated tumor-implanted brain slices. Images with 288 μm × 288 μm size were used for quantification. F. Distribution of vessel diameters (μm) in peritumoral areas after temozolomide (TMZ) treatment and in untreated controls. G. Quantification of vessel length, junctions and branches in glioma implanted untreated slices and temozolomide treated brain slices. Statistical analysis was performed with Student's t -test (* P

    Article Snippet: Primary antibodies laminin (Sigma-Aldrich) were diluted in a concentration of 1:1000 in 3% horse serum (HS) or fetal calf serum (FCS) and 0,2% Triton X-100 as the blocking solution and subsequently incubated for overnight at 4°C.

    Techniques: Staining

    Visualization and quantification of the vasculature in different brain slice areas A. Cortex, hippocampus and white matter were candidate to monitor vascularization in different bran slice areas. Laminin antibody was utilized to stain vessels. Scale bar represents 20 μm. B. Distribution of vessels diameter (μm) in cortex, hippocampus and white matter ( n = 6). C. Quantification of different vascular parameters total vessels length, number of junctions and branches with Image J Angiogenesis tools plugin. Images with 314 μm × 314 μm size were used for quantification. Statistical analysis was performed with Student's t -test (* P

    Journal: Oncotarget

    Article Title: A versatile ex vivo technique for assaying tumor angiogenesis and microglia in the brain

    doi:

    Figure Lengend Snippet: Visualization and quantification of the vasculature in different brain slice areas A. Cortex, hippocampus and white matter were candidate to monitor vascularization in different bran slice areas. Laminin antibody was utilized to stain vessels. Scale bar represents 20 μm. B. Distribution of vessels diameter (μm) in cortex, hippocampus and white matter ( n = 6). C. Quantification of different vascular parameters total vessels length, number of junctions and branches with Image J Angiogenesis tools plugin. Images with 314 μm × 314 μm size were used for quantification. Statistical analysis was performed with Student's t -test (* P

    Article Snippet: Primary antibodies laminin (Sigma-Aldrich) were diluted in a concentration of 1:1000 in 3% horse serum (HS) or fetal calf serum (FCS) and 0,2% Triton X-100 as the blocking solution and subsequently incubated for overnight at 4°C.

    Techniques: Slice Preparation, Staining

    Visualization and quantification of tumor angiogenesis and microglia A. CX3CR1-GFP mouse brain slices were utilized to implant RFP expressing murine glioma cells (GL261). For control samples, we implanted only medium at the same position and in the same manner as tumor was implanted. White squares display peritumoral positions or peri-injection area in sham operated slices which were quantified. Scale bar represents 500 μm. B. Representative image displaying cell death and tumor zones in the VOGIM. Cell nuclei staining is shown in blue (stained with Hoechst 33258), tumor (green) and neurons (red) are given. Arrow indicates the typical breaking-up of neuronal cell structures in the peritumoral zone (TZ II). The tumor bulk is also termed as Tumor Zone I (TZ I). Scale bar represents 100 μm. C. Vascularization in peritumoral area (TZ II). Vessels are stained for Laminin (blue). GFP positive (CX3CR1-GFP mice) cells represent perivascular microglia. Scale bar represents 20 μm. D. Number of perivascular microglial cells in TZ II in comparison to sham. Images with 288 μm × 288 μm size were used for quantification. E. Quantification of vessel parameters: total length, number of junctions and branches in peritumoral area (TZ II) versus sham operated brain slices. Images with 570 μm × 570 μm size were used for quantification ( n = 6). F. Distribution of vessels diameter (μm) in TZ II and sham ( n = 6). G. Images represent tan assembly of pathological tumor vessels found in the VOGIM. Scale bars is given as 40 μm. Statistical analysis was performed with Student's t -test (* P

    Journal: Oncotarget

    Article Title: A versatile ex vivo technique for assaying tumor angiogenesis and microglia in the brain

    doi:

    Figure Lengend Snippet: Visualization and quantification of tumor angiogenesis and microglia A. CX3CR1-GFP mouse brain slices were utilized to implant RFP expressing murine glioma cells (GL261). For control samples, we implanted only medium at the same position and in the same manner as tumor was implanted. White squares display peritumoral positions or peri-injection area in sham operated slices which were quantified. Scale bar represents 500 μm. B. Representative image displaying cell death and tumor zones in the VOGIM. Cell nuclei staining is shown in blue (stained with Hoechst 33258), tumor (green) and neurons (red) are given. Arrow indicates the typical breaking-up of neuronal cell structures in the peritumoral zone (TZ II). The tumor bulk is also termed as Tumor Zone I (TZ I). Scale bar represents 100 μm. C. Vascularization in peritumoral area (TZ II). Vessels are stained for Laminin (blue). GFP positive (CX3CR1-GFP mice) cells represent perivascular microglia. Scale bar represents 20 μm. D. Number of perivascular microglial cells in TZ II in comparison to sham. Images with 288 μm × 288 μm size were used for quantification. E. Quantification of vessel parameters: total length, number of junctions and branches in peritumoral area (TZ II) versus sham operated brain slices. Images with 570 μm × 570 μm size were used for quantification ( n = 6). F. Distribution of vessels diameter (μm) in TZ II and sham ( n = 6). G. Images represent tan assembly of pathological tumor vessels found in the VOGIM. Scale bars is given as 40 μm. Statistical analysis was performed with Student's t -test (* P

    Article Snippet: Primary antibodies laminin (Sigma-Aldrich) were diluted in a concentration of 1:1000 in 3% horse serum (HS) or fetal calf serum (FCS) and 0,2% Triton X-100 as the blocking solution and subsequently incubated for overnight at 4°C.

    Techniques: Expressing, Injection, Staining, Mouse Assay

    Immunohistochemistry of the human spiral ganglion and habenular region. (A) Confocal microscopy demonstrating laminin-β2 and myelin basic protein (MBP) immunoreactivity in human spiral ganglion. Most type I SGNs are MBP-negative. Some non-myelinated perisomal segments show rich expression of laminin (arrow). DAPI, cell nuclei. (B) Confocal microscopy demonstrating laminin-β2 immunoreactivity of basement membrane lining the extracellular surface of the SGCs of the SGN bodies, nerve fibers and blood vessels (BV) (normal audiogram). Type I SGN cell nuclei are round and darkly stained ( ∗ ) while SGC nuclei are crescent-like (arrows) and more lucent. Their cytoplasm shows no laminin expression. DAPI, nuclear staining. BV, blood vessels. (C) Confocal microscopy demonstrating collagen IV immunoreactivity of basement membrane lining the extracellular surface of the SGCs of the SGN bodies, nerve fibers and blood vessels (BV). (D) Confocal microscopy demonstrating laminin-β2 and myelin basic protein (MBP) immunoreactivity in human auditory nerves at the habenula perforata in the organ of Corti. Also blood vessels, Schwann cells and organ of Corti basement membrane show rich expression of laminin. Myelin is lost before neurons pass into the sensory organ. DAPI, cell nuclei. BM, basement membrane. (E) Laminin/TUJ-1 immunohistochemistry of human spiral ganglion. The perineural sheaths stain positive for laminin. Initial segments of the nerve processes generally lack myelin (large arrow). Schwann cell bodies may show rich expression of laminin (small arrow). (F) A rendered confocal microscopy stack showing laminin-β2 immunofluorescence of the human spiral ganglion. A laminin expressing NMSC is seen (thin arrow) as well as a laminin-negative SGC (thick arrow).

    Journal: Neuroscience

    Article Title: The pre- and post-somatic segments of the human type I spiral ganglion neurons – Structural and functional considerations related to cochlear implantation

    doi: 10.1016/j.neuroscience.2014.09.059

    Figure Lengend Snippet: Immunohistochemistry of the human spiral ganglion and habenular region. (A) Confocal microscopy demonstrating laminin-β2 and myelin basic protein (MBP) immunoreactivity in human spiral ganglion. Most type I SGNs are MBP-negative. Some non-myelinated perisomal segments show rich expression of laminin (arrow). DAPI, cell nuclei. (B) Confocal microscopy demonstrating laminin-β2 immunoreactivity of basement membrane lining the extracellular surface of the SGCs of the SGN bodies, nerve fibers and blood vessels (BV) (normal audiogram). Type I SGN cell nuclei are round and darkly stained ( ∗ ) while SGC nuclei are crescent-like (arrows) and more lucent. Their cytoplasm shows no laminin expression. DAPI, nuclear staining. BV, blood vessels. (C) Confocal microscopy demonstrating collagen IV immunoreactivity of basement membrane lining the extracellular surface of the SGCs of the SGN bodies, nerve fibers and blood vessels (BV). (D) Confocal microscopy demonstrating laminin-β2 and myelin basic protein (MBP) immunoreactivity in human auditory nerves at the habenula perforata in the organ of Corti. Also blood vessels, Schwann cells and organ of Corti basement membrane show rich expression of laminin. Myelin is lost before neurons pass into the sensory organ. DAPI, cell nuclei. BM, basement membrane. (E) Laminin/TUJ-1 immunohistochemistry of human spiral ganglion. The perineural sheaths stain positive for laminin. Initial segments of the nerve processes generally lack myelin (large arrow). Schwann cell bodies may show rich expression of laminin (small arrow). (F) A rendered confocal microscopy stack showing laminin-β2 immunofluorescence of the human spiral ganglion. A laminin expressing NMSC is seen (thin arrow) as well as a laminin-negative SGC (thick arrow).

    Article Snippet: Antibody and IHC The antibody against laminin-β2 was a monoclonal antibody from the rat (catalog number #05-206, Millipore, Billerica, MA, USA; dilution 1:100).

    Techniques: Immunohistochemistry, Confocal Microscopy, Expressing, Staining, Immunofluorescence

    Immunohistochemical staining of basement membrane components, including laminin 1 (a), laminin 5 (b), collagen type IV (c) and fibronectin (d) in a granular cell ameloblastoma. ( a ) An intense staining of laminin 1 is found in the peripheral cells, central stellate shaped-like cells and granular cells. ( b ) A moderate-to-strong intensity of laminin 5 is seen in granular cells. ( c ) A strong expression of collagen type IV is detected at the basement membrane area surrounding tumor follicles. ( d ) A weak to moderate staining for fibronectin is observed in peripheral cells and central cells, whereas a moderate-to-strong staining is present in granular cells (immunostaining, original magnification: ×200).

    Journal: International Journal of Oral Science

    Article Title: Investigation of basement membrane proteins in a case of granular cell ameloblastoma

    doi: 10.1038/ijos.2012.9

    Figure Lengend Snippet: Immunohistochemical staining of basement membrane components, including laminin 1 (a), laminin 5 (b), collagen type IV (c) and fibronectin (d) in a granular cell ameloblastoma. ( a ) An intense staining of laminin 1 is found in the peripheral cells, central stellate shaped-like cells and granular cells. ( b ) A moderate-to-strong intensity of laminin 5 is seen in granular cells. ( c ) A strong expression of collagen type IV is detected at the basement membrane area surrounding tumor follicles. ( d ) A weak to moderate staining for fibronectin is observed in peripheral cells and central cells, whereas a moderate-to-strong staining is present in granular cells (immunostaining, original magnification: ×200).

    Article Snippet: Endogenous peroxidase was then blocked with 3% hydrogen peroxide for 10 min. For antibodies against laminin 1, collagen type IV and fibronectin, antigen retrieval was performed by incubating the sections with 0.4% pepsin (Sigma Chemical Co., St Louis, MO, USA) in 0.01 mol·L−1 HCl at 37 °C for 1 h. For antibodies against laminin 5, antigen retrieval was done by covering the sections with proteinase K (Dako Corporation, Carpinteria, CA, USA) at room temperature for 15 min. After washing with 0.1% Tween 20 (MERCK-Schuchardt, Hohanbrumm, Germany) in phosphate-buffered saline, the sections were treated with 2% bovine serum albumin (Sigma Chemical Co.) in phosphate-buffered saline for 30 min and then incubated with primary antibodies diluted in phosphate-buffered saline for 2 h at room temperature.

    Techniques: Immunohistochemistry, Staining, Expressing, Immunostaining

    Diaphragm formation during mouse development. ( A ) Sagittal sections of E15.5 wt, Igf2 +/- , Myod -/- and DM embryos were immunostained for Pax7 (α-Pax7, green) and laminin (α-Laminin, red). Myod -/- and DM diaphragms are thinner than those

    Journal: Development (Cambridge, England)

    Article Title: Myod and H19-Igf2 locus interactions are required for diaphragm formation in the mouse

    doi: 10.1242/dev.084665

    Figure Lengend Snippet: Diaphragm formation during mouse development. ( A ) Sagittal sections of E15.5 wt, Igf2 +/- , Myod -/- and DM embryos were immunostained for Pax7 (α-Pax7, green) and laminin (α-Laminin, red). Myod -/- and DM diaphragms are thinner than those

    Article Snippet: For assessment of tissue morphology, 5 μm transverse sections were stained with H & E. Fiber size was analyzed by immunostaining muscle sections with an antibody to laminin (Novocastra) and counterstaining with DAPI.

    Techniques: