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Image Search Results
Journal: Frontiers in Oncology
Article Title: Human Epididymis Protein 4 Promotes Events Associated with Metastatic Ovarian Cancer via Regulation of the Extracelluar Matrix
doi: 10.3389/fonc.2017.00332
Figure Lengend Snippet: Transcripts differentially expressed between OVCAR8-NV/OVCAR8-C5 (A), and OVCAR8-wild-type (WT)-untreated/OVCAR8-WT-recombinant human epididymis protein 4 (rHE4) treated (B) cells.
Article Snippet: Antibodies and dilutions used are as follows:
Techniques: Sequencing, RNA Binding Assay, Binding Assay
Journal: Frontiers in Oncology
Article Title: Human Epididymis Protein 4 Promotes Events Associated with Metastatic Ovarian Cancer via Regulation of the Extracelluar Matrix
doi: 10.3389/fonc.2017.00332
Figure Lengend Snippet: Transcripts differentially expressed with both human epididymis protein 4 (HE4) overexpression and recombinant HE4 (rHE4) treatment.
Article Snippet: Antibodies and dilutions used are as follows:
Techniques: Over Expression, Recombinant, Zinc-Fingers, Sequencing, Binding Assay
Journal: Frontiers in Oncology
Article Title: Human Epididymis Protein 4 Promotes Events Associated with Metastatic Ovarian Cancer via Regulation of the Extracelluar Matrix
doi: 10.3389/fonc.2017.00332
Figure Lengend Snippet: Quantitative reverse transcription polymerase chain reaction validation of microarray SERPINB2, GREM1, LAMC2, LAMB3 , and TNC were selected to validate microarray results in OVCAR8-NV versus OVCAR8-C5 (A) and OVCAR8-wild-type (WT) untreated versus recombinant human epididymis protein 4 (rHE4)-treated (B) . Error bars represent the SD of three biological replicates, * p < 0.05, ** p < 0.005, and *** p < 0.0005 versus controls.
Article Snippet: Antibodies and dilutions used are as follows:
Techniques: Reverse Transcription, Polymerase Chain Reaction, Biomarker Discovery, Microarray, Recombinant
Journal: Frontiers in Oncology
Article Title: Human Epididymis Protein 4 Promotes Events Associated with Metastatic Ovarian Cancer via Regulation of the Extracelluar Matrix
doi: 10.3389/fonc.2017.00332
Figure Lengend Snippet: Human epididymis protein 4 (HE4) promotes an increase in laminin-332. (A) Protein levels of LAMC2 and LAMB3 in OVCAR8-NV and OVCAR8-C5 cells were determined by Western blot. GAPDH was used as a loading control. (B,C) Densitometry analysis of LAMC2 and LAMB3 (normalized to GAPDH) from (A) . (D,E) Time course analysis of protein levels in OVCAR8-wild-type (WT) cells left untreated or treated with 20 nM recombinant HE4 (rHE4) or 50% conditioned media from OVCAR8-C5 cells. (F,G) Densitometry analysis of LAMC2 and LAMB3 (normalized to GAPDH) from Western blot in (D,E) . (H) OVCAR8 cells were treated with rHE4 or conditioned media from OVCAR8-C5 cells for 5 h, and immunofluorescence staining for laminin-332 was performed. Representative images are shown. Green = laminin-332, blue = DAPI, scale = 40×. (I) Intensity was determined for laminin-332. Results are the average of fold change from three independent experiments with three replicates per experiment and four fields for each replicate. Error bars indicate standard error of the mean. ** p < 0.005 versus control.
Article Snippet: Antibodies and dilutions used are as follows:
Techniques: Western Blot, Control, Recombinant, Immunofluorescence, Staining