lamc2 Search Results


lamc2  (Proteintech)
94
Proteintech lamc2
Figure 3. miR-212-5p negatively regulates <t>LAMC2</t> and LAMA3: (A) miRanda (v3.3a) and TargetScan (v5.0) projected miR-212-5p target genes displayed; (B) similarity of rno-miR-212-5p and has-miR- 212-5p genes shown; (C) schematic representation of LAMC2-3′ UTR and LAMA3-3′ UTR with miR- 212-5p target prediction and reporter plasmid construction, where red characters indicate binding sites and ”*” characters indicate mutation sites; (D) luciferase activity assessed in 293T cells after co- transfection of LAMC2-WT, LAMC2-MUT, LAMA3-WT, and LAMA3-MUT with miR-212-5p mimics or miR-mimics NC for 48 h; (E) LAMC2-mVenus-WT, LAMC2-mVenus-MUT, LAMA3-mVenus-WT, and LAMA3-mVenus-MUT co-transfected with miR-mimics NC or miR-212-5p in 293T cells (scale bar: 300 µm); (F) RT-PCR assay for miR-212-5p mimics and miR-212-5p inhibitors on LAMC2 and LAMA3 expression after transfection; (G) Western blotting used to identify alterations in the LAMC2 and LAMA3 proteins. The mean ± standard deviation (n = 3) is used to express the data. ns, no significance; *, p < 0.05; **, p < 0.01; and ***, p < 0.001 indicate significance.
Lamc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pm40004224-251-29-37?v=Proteintech
Average 94 stars, based on 1 article reviews
lamc2 - by Bioz Stars, 2026-08
94/100 stars
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cl2980  (Novus Biologicals)
93
Novus Biologicals cl2980
KEY RESOURCES TABLE
Cl2980, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc05878932-8-4-8?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
cl2980 - by Bioz Stars, 2026-08
93/100 stars
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anti lamc2  (Atlas Antibodies)
92
Atlas Antibodies anti lamc2
KEY RESOURCES TABLE
Anti Lamc2, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pm37640197-64-25-26?v=Atlas+Antibodies
Average 92 stars, based on 1 article reviews
anti lamc2 - by Bioz Stars, 2026-08
92/100 stars
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gene exp lamc2 hs01043711 m1  (Thermo Fisher)
94
Thermo Fisher gene exp lamc2 hs01043711 m1
Basement membrane protein expression in laser-capture microdissected epidermis and dermis before and during wound healing in young adult human skin. a Unwounded skin baseline levels of COL4A2, COL7A2, and <t>LAMC2</t> transcripts in epidermis (light gray) and dermis (dark gray) separated by laser capture microdissection. *: p < .05 vs. Epidermis (Wilcoxon signed rank test). b-d Normalized transcript levels in epidermis following wounding (expressed in fold vs. 36B4 (housekeeping gene)) for COL4A2 (b), COL7A2 (c) and LAMC2 (d). N = 3–6 sample per time point. *: p < .05 vs. Epidermis (paired t-test)
Gene Exp Lamc2 Hs01043711 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc05842181-76-17-5?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
gene exp lamc2 hs01043711 m1 - by Bioz Stars, 2026-08
94/100 stars
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gene exp lamc2 hs01043717 m1  (Thermo Fisher)
85
Thermo Fisher gene exp lamc2 hs01043717 m1
Genes that were highly up- or down-regulated in KVECs compared to in NVECs.
Gene Exp Lamc2 Hs01043717 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc07403211-128-40--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp lamc2 hs01043717 m1 - by Bioz Stars, 2026-08
85/100 stars
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gene exp lamc2 hs00194345 m1  (Thermo Fisher)
94
Thermo Fisher gene exp lamc2 hs00194345 m1
Genes that were highly up- or down-regulated in KVECs compared to in NVECs.
Gene Exp Lamc2 Hs00194345 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc12300802-51-38-11?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
gene exp lamc2 hs00194345 m1 - by Bioz Stars, 2026-08
94/100 stars
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elisa—enzyme-linked immunosorbent assays for lamc2, dsg2, dsp, and gp73  (USCN Life)
90
USCN Life elisa—enzyme-linked immunosorbent assays for lamc2, dsg2, dsp, and gp73
Genes that were highly up- or down-regulated in KVECs compared to in NVECs.
Elisa—Enzyme Linked Immunosorbent Assays For Lamc2, Dsg2, Dsp, And Gp73, supplied by USCN Life, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/10__1074_slash_mcp__m112__023507-103-8-12?v=USCN+Life
Average 90 stars, based on 1 article reviews
elisa—enzyme-linked immunosorbent assays for lamc2, dsg2, dsp, and gp73 - by Bioz Stars, 2026-08
90/100 stars
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lamc2 monoclonal antibody yt5379  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company lamc2 monoclonal antibody yt5379
Primer sequences for quantitative real-time polymerase chain reaction
Lamc2 Monoclonal Antibody Yt5379, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc09516679-72-5-9?v=ImmunoWay+Biotechnology+Company
Average 90 stars, based on 1 article reviews
lamc2 monoclonal antibody yt5379 - by Bioz Stars, 2026-08
90/100 stars
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anti-lamc2 antibody  (GeneTex)
90
GeneTex anti-lamc2 antibody
Primer sequences for quantitative real-time polymerase chain reaction
Anti Lamc2 Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc07403211-135-14-16?v=GeneTex
Average 90 stars, based on 1 article reviews
anti-lamc2 antibody - by Bioz Stars, 2026-08
90/100 stars
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anti-lamc2 usb-144830  (US Biological Life Sciences)
90
US Biological Life Sciences anti-lamc2 usb-144830
The UGT1A3 expression plasmid was transfected into UGT1A3-KO cells (UGT-KO), which were designated UGT-KO + UGT over, and then the <t>LAMC2</t> knockdown plasmid was transfected into UGT-KO + UGT over cells, which were designated UGT-KO + UGT over+ si-LAM. A Differential expression levels of genes between UGT-KO and NC cell lines are displayed by a heat map. B Volcano plots of differentially expressed gene expression. The green or red plots are the DEGs (fold change >4 and Padj < 0.05). C DEGs were analyzed by KEGG pathway enrichment analysis. D The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO and NC cell lines. E The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO, UGT-KO + UGT over and UGT-KO + UGT over+ si-LAM cell lines. F Cell viability was detected using a CCK-8 assay (OD450 absorbance). G The colony formation ability was measured using a colony formation assay. The colonies were counted and captured. H Representative images of invasion (upper) and migration assays (lower). The number of cells was counted. Scale bar, 180 μm. I The cell cycle was measured by flow cytometry. *P < 0.05 according to Student’s t test.
Anti Lamc2 Usb 144830, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc08016664-369-29-32?v=US+Biological+Life+Sciences
Average 90 stars, based on 1 article reviews
anti-lamc2 usb-144830 - by Bioz Stars, 2026-08
90/100 stars
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lamc2-sirna constructs  (Ribobio co)
90
Ribobio co lamc2-sirna constructs
The UGT1A3 expression plasmid was transfected into UGT1A3-KO cells (UGT-KO), which were designated UGT-KO + UGT over, and then the <t>LAMC2</t> knockdown plasmid was transfected into UGT-KO + UGT over cells, which were designated UGT-KO + UGT over+ si-LAM. A Differential expression levels of genes between UGT-KO and NC cell lines are displayed by a heat map. B Volcano plots of differentially expressed gene expression. The green or red plots are the DEGs (fold change >4 and Padj < 0.05). C DEGs were analyzed by KEGG pathway enrichment analysis. D The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO and NC cell lines. E The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO, UGT-KO + UGT over and UGT-KO + UGT over+ si-LAM cell lines. F Cell viability was detected using a CCK-8 assay (OD450 absorbance). G The colony formation ability was measured using a colony formation assay. The colonies were counted and captured. H Representative images of invasion (upper) and migration assays (lower). The number of cells was counted. Scale bar, 180 μm. I The cell cycle was measured by flow cytometry. *P < 0.05 according to Student’s t test.
Lamc2 Sirna Constructs, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc2/pmc11399656-325-0-5?v=Ribobio+co
Average 90 stars, based on 1 article reviews
lamc2-sirna constructs - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Figure 3. miR-212-5p negatively regulates LAMC2 and LAMA3: (A) miRanda (v3.3a) and TargetScan (v5.0) projected miR-212-5p target genes displayed; (B) similarity of rno-miR-212-5p and has-miR- 212-5p genes shown; (C) schematic representation of LAMC2-3′ UTR and LAMA3-3′ UTR with miR- 212-5p target prediction and reporter plasmid construction, where red characters indicate binding sites and ”*” characters indicate mutation sites; (D) luciferase activity assessed in 293T cells after co- transfection of LAMC2-WT, LAMC2-MUT, LAMA3-WT, and LAMA3-MUT with miR-212-5p mimics or miR-mimics NC for 48 h; (E) LAMC2-mVenus-WT, LAMC2-mVenus-MUT, LAMA3-mVenus-WT, and LAMA3-mVenus-MUT co-transfected with miR-mimics NC or miR-212-5p in 293T cells (scale bar: 300 µm); (F) RT-PCR assay for miR-212-5p mimics and miR-212-5p inhibitors on LAMC2 and LAMA3 expression after transfection; (G) Western blotting used to identify alterations in the LAMC2 and LAMA3 proteins. The mean ± standard deviation (n = 3) is used to express the data. ns, no significance; *, p < 0.05; **, p < 0.01; and ***, p < 0.001 indicate significance.

Journal: International journal of molecular sciences

Article Title: miR-212-5p Regulates PM 2.5 -Induced Apoptosis by Targeting LAMC2 and LAMA3.

doi: 10.3390/ijms26041761

Figure Lengend Snippet: Figure 3. miR-212-5p negatively regulates LAMC2 and LAMA3: (A) miRanda (v3.3a) and TargetScan (v5.0) projected miR-212-5p target genes displayed; (B) similarity of rno-miR-212-5p and has-miR- 212-5p genes shown; (C) schematic representation of LAMC2-3′ UTR and LAMA3-3′ UTR with miR- 212-5p target prediction and reporter plasmid construction, where red characters indicate binding sites and ”*” characters indicate mutation sites; (D) luciferase activity assessed in 293T cells after co- transfection of LAMC2-WT, LAMC2-MUT, LAMA3-WT, and LAMA3-MUT with miR-212-5p mimics or miR-mimics NC for 48 h; (E) LAMC2-mVenus-WT, LAMC2-mVenus-MUT, LAMA3-mVenus-WT, and LAMA3-mVenus-MUT co-transfected with miR-mimics NC or miR-212-5p in 293T cells (scale bar: 300 µm); (F) RT-PCR assay for miR-212-5p mimics and miR-212-5p inhibitors on LAMC2 and LAMA3 expression after transfection; (G) Western blotting used to identify alterations in the LAMC2 and LAMA3 proteins. The mean ± standard deviation (n = 3) is used to express the data. ns, no significance; *, p < 0.05; **, p < 0.01; and ***, p < 0.001 indicate significance.

Article Snippet: Next, 5% skimmed milk (Beyotime Biotechnology, Shanghai, China) was blocked for 2 h, and the membranes were incubated with specific primary antibodies overnight at 4 ◦C: BAD, BCL-2, caspase-3, LAMC2, p85, AKT, p-AKT, IκBα, β-action (1:1000 dilution, Proteintech, Rosemont, IL, USA), p-IκBα, p65, p-p65 (1:500 dilution, Proteintech, IL, USA), LAMA3, p-p85 (1:500 dilution, Abways, Shanghai, China), and GAPDH (1:7000 dilution, Abways, Shanghai, China).

Techniques: Plasmid Preparation, Binding Assay, Mutagenesis, Luciferase, Activity Assay, Cotransfection, Transfection, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Standard Deviation

Figure 4. PM2.5-induced changes in LAMC2 and LAMA3 expression: (A) LAMC2 and LAMA3 gene expression detected with RT-PCR after PM2.5 stimulation; (B) LAMC2 and LAMA3 protein changes detected with Western blotting; (C) silencing efficiency of LAMC2 and LAMA3 after transfection with siRNA−LAMC2 or siRNA−LAMA3 detected with RT-PCR; (D) LAMC2 and LAMA3 protein changes detected with Western blotting. The mean ± standard deviation (n = 3) is used to express the data. ns, no significance; *, p < 0.05; **, p < 0.01; and ***, p < 0.001 indicate significance.

Journal: International journal of molecular sciences

Article Title: miR-212-5p Regulates PM 2.5 -Induced Apoptosis by Targeting LAMC2 and LAMA3.

doi: 10.3390/ijms26041761

Figure Lengend Snippet: Figure 4. PM2.5-induced changes in LAMC2 and LAMA3 expression: (A) LAMC2 and LAMA3 gene expression detected with RT-PCR after PM2.5 stimulation; (B) LAMC2 and LAMA3 protein changes detected with Western blotting; (C) silencing efficiency of LAMC2 and LAMA3 after transfection with siRNA−LAMC2 or siRNA−LAMA3 detected with RT-PCR; (D) LAMC2 and LAMA3 protein changes detected with Western blotting. The mean ± standard deviation (n = 3) is used to express the data. ns, no significance; *, p < 0.05; **, p < 0.01; and ***, p < 0.001 indicate significance.

Article Snippet: Next, 5% skimmed milk (Beyotime Biotechnology, Shanghai, China) was blocked for 2 h, and the membranes were incubated with specific primary antibodies overnight at 4 ◦C: BAD, BCL-2, caspase-3, LAMC2, p85, AKT, p-AKT, IκBα, β-action (1:1000 dilution, Proteintech, Rosemont, IL, USA), p-IκBα, p65, p-p65 (1:500 dilution, Proteintech, IL, USA), LAMA3, p-p85 (1:500 dilution, Abways, Shanghai, China), and GAPDH (1:7000 dilution, Abways, Shanghai, China).

Techniques: Expressing, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection, Standard Deviation

Figure 5. LAMC2 and LAMA3 inhibit apoptosis through the PI3K/AKT/NF-κB pathway: (A) PM2.5- stimulated (180 µg/mL for 24 h) cells transfected with siRNA−LAMC2 or siRNA−LAMA3, and p-IκBα/IκBα and p-p65/p65 protein changes; (B) p-AKT/AKT and p-p85/p85 protein changes; (C) BCL-2, BAD, and caspase-3 protein changes detected with Western blotting; (D) apoptosis rate detected with flow cytometry. The mean ± standard deviation (n = 3) is used to express the data. ns, no significance; *, p < 0.05; **, p < 0.01; and ***, p < 0.001 indicate significance.

Journal: International journal of molecular sciences

Article Title: miR-212-5p Regulates PM 2.5 -Induced Apoptosis by Targeting LAMC2 and LAMA3.

doi: 10.3390/ijms26041761

Figure Lengend Snippet: Figure 5. LAMC2 and LAMA3 inhibit apoptosis through the PI3K/AKT/NF-κB pathway: (A) PM2.5- stimulated (180 µg/mL for 24 h) cells transfected with siRNA−LAMC2 or siRNA−LAMA3, and p-IκBα/IκBα and p-p65/p65 protein changes; (B) p-AKT/AKT and p-p85/p85 protein changes; (C) BCL-2, BAD, and caspase-3 protein changes detected with Western blotting; (D) apoptosis rate detected with flow cytometry. The mean ± standard deviation (n = 3) is used to express the data. ns, no significance; *, p < 0.05; **, p < 0.01; and ***, p < 0.001 indicate significance.

Article Snippet: Next, 5% skimmed milk (Beyotime Biotechnology, Shanghai, China) was blocked for 2 h, and the membranes were incubated with specific primary antibodies overnight at 4 ◦C: BAD, BCL-2, caspase-3, LAMC2, p85, AKT, p-AKT, IκBα, β-action (1:1000 dilution, Proteintech, Rosemont, IL, USA), p-IκBα, p65, p-p65 (1:500 dilution, Proteintech, IL, USA), LAMA3, p-p85 (1:500 dilution, Abways, Shanghai, China), and GAPDH (1:7000 dilution, Abways, Shanghai, China).

Techniques: Transfection, Western Blot, Flow Cytometry, Standard Deviation

KEY RESOURCES TABLE

Journal: Cell

Article Title: Single-cell transcriptomic analysis of primary and metastatic tumor ecosystems in head and neck cancer

doi: 10.1016/j.cell.2017.10.044

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Monoclonal mouse LAMC2, clone CL2980, lot #CL2980 , Novus Biologicals , Cat#NBP2-42388.

Techniques: RNAscope, Labeling, Virus, Recombinant, Cloning, Transfection, Plasmid Preparation, Control, Software

Basement membrane protein expression in laser-capture microdissected epidermis and dermis before and during wound healing in young adult human skin. a Unwounded skin baseline levels of COL4A2, COL7A2, and LAMC2 transcripts in epidermis (light gray) and dermis (dark gray) separated by laser capture microdissection. *: p < .05 vs. Epidermis (Wilcoxon signed rank test). b-d Normalized transcript levels in epidermis following wounding (expressed in fold vs. 36B4 (housekeeping gene)) for COL4A2 (b), COL7A2 (c) and LAMC2 (d). N = 3–6 sample per time point. *: p < .05 vs. Epidermis (paired t-test)

Journal: Journal of Cell Communication and Signaling

Article Title: Restoration of the basement membrane after wounding: a hallmark of young human skin altered with aging

doi: 10.1007/s12079-017-0417-3

Figure Lengend Snippet: Basement membrane protein expression in laser-capture microdissected epidermis and dermis before and during wound healing in young adult human skin. a Unwounded skin baseline levels of COL4A2, COL7A2, and LAMC2 transcripts in epidermis (light gray) and dermis (dark gray) separated by laser capture microdissection. *: p < .05 vs. Epidermis (Wilcoxon signed rank test). b-d Normalized transcript levels in epidermis following wounding (expressed in fold vs. 36B4 (housekeeping gene)) for COL4A2 (b), COL7A2 (c) and LAMC2 (d). N = 3–6 sample per time point. *: p < .05 vs. Epidermis (paired t-test)

Article Snippet: Primer/Probe pairs were obtained from Applied Biosystems (ThermoFisher Scientific) as follows: COL4A2 (Hs01098873_m1), COL7A1 (Hs00164310_m1), and LAMC2 (Hs01043711_m1).

Techniques: Membrane, Expressing, Laser Capture Microdissection

Basement membrane protein gene expression in laser-capture microdissected epidermis and dermis of young and aged human skin during wound healing. mRNA levels of LAMC2 (a, d), COL7A2 (b, e), and COL4A2 (c, f) in epidermis (a-c) and dermis (d-f) following wounding (expressed in fold change vs. unwounded baseline). N = 3–6 per group. *: p < .05 vs. Young (unpaired t-test)

Journal: Journal of Cell Communication and Signaling

Article Title: Restoration of the basement membrane after wounding: a hallmark of young human skin altered with aging

doi: 10.1007/s12079-017-0417-3

Figure Lengend Snippet: Basement membrane protein gene expression in laser-capture microdissected epidermis and dermis of young and aged human skin during wound healing. mRNA levels of LAMC2 (a, d), COL7A2 (b, e), and COL4A2 (c, f) in epidermis (a-c) and dermis (d-f) following wounding (expressed in fold change vs. unwounded baseline). N = 3–6 per group. *: p < .05 vs. Young (unpaired t-test)

Article Snippet: Primer/Probe pairs were obtained from Applied Biosystems (ThermoFisher Scientific) as follows: COL4A2 (Hs01098873_m1), COL7A1 (Hs00164310_m1), and LAMC2 (Hs01043711_m1).

Techniques: Membrane, Gene Expression

Genes that were highly up- or down-regulated in KVECs compared to in NVECs.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Gene Expression Profile of Isolated Dermal Vascular Endothelial Cells in Keloids

doi: 10.3389/fcell.2020.00658

Figure Lengend Snippet: Genes that were highly up- or down-regulated in KVECs compared to in NVECs.

Article Snippet: cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qRT-PCR assays were performed using an ABI Prism 7500 System (Applied Biosystems, Foster City, CA, United States) with Applied Biosystems TaqMan Gene Expression Assays (SERPINA3, Hs00153674_m1; LAMC2, Hs01043717_m1; GAPDH, Hs03929097_g1) and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific).

Techniques: Sequencing, Membrane

Network analysis with the 74 and 80 genes that were, respectively, up- and down-regulated by more than 5-fold in keloid vascular endothelial cells. SERPINA3 (red) and LAMC2 (yellow) are located near the center of the network.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Gene Expression Profile of Isolated Dermal Vascular Endothelial Cells in Keloids

doi: 10.3389/fcell.2020.00658

Figure Lengend Snippet: Network analysis with the 74 and 80 genes that were, respectively, up- and down-regulated by more than 5-fold in keloid vascular endothelial cells. SERPINA3 (red) and LAMC2 (yellow) are located near the center of the network.

Article Snippet: cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qRT-PCR assays were performed using an ABI Prism 7500 System (Applied Biosystems, Foster City, CA, United States) with Applied Biosystems TaqMan Gene Expression Assays (SERPINA3, Hs00153674_m1; LAMC2, Hs01043717_m1; GAPDH, Hs03929097_g1) and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific).

Techniques:

Validation of the microarray data by quantitative RT-PCR. The relative mRNA expression levels of SERPINA3 (A) and LAMC2 (B) in keloid vascular endothelial cells (KVECs) ( n = 7) and normal vascular endothelial cells (NVECs) ( n = 6) are shown. All values in this figure represent the mean ± SD.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Gene Expression Profile of Isolated Dermal Vascular Endothelial Cells in Keloids

doi: 10.3389/fcell.2020.00658

Figure Lengend Snippet: Validation of the microarray data by quantitative RT-PCR. The relative mRNA expression levels of SERPINA3 (A) and LAMC2 (B) in keloid vascular endothelial cells (KVECs) ( n = 7) and normal vascular endothelial cells (NVECs) ( n = 6) are shown. All values in this figure represent the mean ± SD.

Article Snippet: cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qRT-PCR assays were performed using an ABI Prism 7500 System (Applied Biosystems, Foster City, CA, United States) with Applied Biosystems TaqMan Gene Expression Assays (SERPINA3, Hs00153674_m1; LAMC2, Hs01043717_m1; GAPDH, Hs03929097_g1) and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific).

Techniques: Biomarker Discovery, Microarray, Quantitative RT-PCR, Expressing

Immunohistochemical validation of (A) SERPINA3 and (B) LAMC2 protein expression in the blood vessels of keloid and normal skin tissues. Representative histological images are shown. Arrows indicate the blood vessels in the dermis. LPF, low-power field, HPF, high-power field. Scale bars: LPF, 50 μm, HPF, 20 μm.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Gene Expression Profile of Isolated Dermal Vascular Endothelial Cells in Keloids

doi: 10.3389/fcell.2020.00658

Figure Lengend Snippet: Immunohistochemical validation of (A) SERPINA3 and (B) LAMC2 protein expression in the blood vessels of keloid and normal skin tissues. Representative histological images are shown. Arrows indicate the blood vessels in the dermis. LPF, low-power field, HPF, high-power field. Scale bars: LPF, 50 μm, HPF, 20 μm.

Article Snippet: cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qRT-PCR assays were performed using an ABI Prism 7500 System (Applied Biosystems, Foster City, CA, United States) with Applied Biosystems TaqMan Gene Expression Assays (SERPINA3, Hs00153674_m1; LAMC2, Hs01043717_m1; GAPDH, Hs03929097_g1) and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific).

Techniques: Immunohistochemical staining, Biomarker Discovery, Expressing

In vitro study on the biological functions of SERPINA3 and LAMC2 in vascular endothelial cells and fibroblasts. (A) A human dermal vascular endothelial cell line (HMEC-1) was treated with or without 1 μg/ml lipopolysaccharide (LPS) for 15 h ( n = 6). The relative mRNA expression levels of SERPINA3 and LAMC2 in the cells was determined. (B) Normal human dermal fibroblasts (NHDF) were treated with or without 0.5 μg/ml laminin-5 for 15 h ( n = 10). The relative cell proliferation and apoptosis was measured. All values in this figure represent the mean ± SD. ** p < 0.01.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Gene Expression Profile of Isolated Dermal Vascular Endothelial Cells in Keloids

doi: 10.3389/fcell.2020.00658

Figure Lengend Snippet: In vitro study on the biological functions of SERPINA3 and LAMC2 in vascular endothelial cells and fibroblasts. (A) A human dermal vascular endothelial cell line (HMEC-1) was treated with or without 1 μg/ml lipopolysaccharide (LPS) for 15 h ( n = 6). The relative mRNA expression levels of SERPINA3 and LAMC2 in the cells was determined. (B) Normal human dermal fibroblasts (NHDF) were treated with or without 0.5 μg/ml laminin-5 for 15 h ( n = 10). The relative cell proliferation and apoptosis was measured. All values in this figure represent the mean ± SD. ** p < 0.01.

Article Snippet: cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qRT-PCR assays were performed using an ABI Prism 7500 System (Applied Biosystems, Foster City, CA, United States) with Applied Biosystems TaqMan Gene Expression Assays (SERPINA3, Hs00153674_m1; LAMC2, Hs01043717_m1; GAPDH, Hs03929097_g1) and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific).

Techniques: In Vitro, Expressing

Primer sequences for quantitative real-time polymerase chain reaction

Journal: World Journal of Gastroenterology

Article Title: Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1

doi: 10.3748/wjg.v28.i35.5154

Figure Lengend Snippet: Primer sequences for quantitative real-time polymerase chain reaction

Article Snippet: The following antibodies were used: LAMC2 monoclonal antibody (YT5379, Immunoway, United States), ITGB1 monoclonal antibody (sc-9970, Santa Cruz, United States), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody (ab125247, ABCAM, United Kingdom), phosphorylate-FAK (p - FAK)-Y397 (AP0302, ABclonal, Wuhan, China), FAK monoclonal antibody (A11131, ABclonal, Wuhan, China), snail monoclonal antibody (ab53519, ABCAM, United Kingdom), E-cadherin polyclonal antibody (ab1416, ABCAM, United Kingdom), N-cadherin polyclonal antibody (22018-1-AP, Proteintech, United States), fibronectin polyclonal antibody (A16678, ABclonal, Wuhan, China) and special AT-rich sequence binding protein 1 (SATB1) polyclonal antibody (A5800, ABclonal, Wuhan, China).

Techniques:

Alcohol consumption regulates the expression and interaction of integrin-β1 and laminin-γ2 in animal samples. The bars represent the mean ± SD ( n = 8). A and B: Quantification of the protein levels of integrin-β1 (ITGB1) and laminin-γ2 (LAMC2) detected by western blotting in animal samples; C and D: Quantification of the protein levels of ITGB1 and LAMC2 by immunohistochemical (IHC) in animal samples; E: Quantification of the mRNA levels of ITGB1 and LAMC2 by quantitative real-time polymerase chain reaction in clinical samples; F: Statistical analysis of IHC was performed by counting the average optical density (AOD). In Duolink proximity ligation assay (PLA), the brown dot particles observed under the microscope represent the interactions between LAMC2 and ITGB1; G: The statistical analysis of PLA positive signals was performed by counting the AOD under a light microscope. Compared with the non-alcohol group: b P < 0.01, a P < 0.05. ITGB1: Integrin-β1; LAMC2: Laminin-γ2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; PLA: Proximity ligation assay; IHC: Immunohistochemical staining; AOD: Average optical density.

Journal: World Journal of Gastroenterology

Article Title: Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1

doi: 10.3748/wjg.v28.i35.5154

Figure Lengend Snippet: Alcohol consumption regulates the expression and interaction of integrin-β1 and laminin-γ2 in animal samples. The bars represent the mean ± SD ( n = 8). A and B: Quantification of the protein levels of integrin-β1 (ITGB1) and laminin-γ2 (LAMC2) detected by western blotting in animal samples; C and D: Quantification of the protein levels of ITGB1 and LAMC2 by immunohistochemical (IHC) in animal samples; E: Quantification of the mRNA levels of ITGB1 and LAMC2 by quantitative real-time polymerase chain reaction in clinical samples; F: Statistical analysis of IHC was performed by counting the average optical density (AOD). In Duolink proximity ligation assay (PLA), the brown dot particles observed under the microscope represent the interactions between LAMC2 and ITGB1; G: The statistical analysis of PLA positive signals was performed by counting the AOD under a light microscope. Compared with the non-alcohol group: b P < 0.01, a P < 0.05. ITGB1: Integrin-β1; LAMC2: Laminin-γ2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; PLA: Proximity ligation assay; IHC: Immunohistochemical staining; AOD: Average optical density.

Article Snippet: The following antibodies were used: LAMC2 monoclonal antibody (YT5379, Immunoway, United States), ITGB1 monoclonal antibody (sc-9970, Santa Cruz, United States), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody (ab125247, ABCAM, United Kingdom), phosphorylate-FAK (p - FAK)-Y397 (AP0302, ABclonal, Wuhan, China), FAK monoclonal antibody (A11131, ABclonal, Wuhan, China), snail monoclonal antibody (ab53519, ABCAM, United Kingdom), E-cadherin polyclonal antibody (ab1416, ABCAM, United Kingdom), N-cadherin polyclonal antibody (22018-1-AP, Proteintech, United States), fibronectin polyclonal antibody (A16678, ABclonal, Wuhan, China) and special AT-rich sequence binding protein 1 (SATB1) polyclonal antibody (A5800, ABclonal, Wuhan, China).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Real-time Polymerase Chain Reaction, Proximity Ligation Assay, Microscopy, Light Microscopy, Staining

Alcohol consumption increased the expression and interaction of laminin-γ2 and integrin-β1 in colorectal cancer rats. The bars represent the mean ± SD ( n = 8). A: Survival analysis of integrin-β1 (ITGB1) and laminin-γ2 (LAMC2) in colorectal cancer; B: The mRNA levels of ITGB1 and LAMC2 determined by quantitative real-time polymerase chain reaction in clinical samples; C: Representative immunohistochemical (IHC) images of ITGB1 and LAMC2 in clinical samples (× 400); D: The quantifications of the protein levels of ITGB1 and LAMC2 determined by IHC in clinical samples; E and F: Duolink proximity ligation assay (PLA) verified the interactions between ITGB1 and LAMC2 in clinical samples. The statistical analysis of PLA positive signals was performed by counting the average optical density under a light microscope. Compared with the non-alcohol group: b P < 0.01, a P < 0.05. LAMC2: Laminin-γ2; ITGB1: Integrin-β1; qRT-PCR: Quantitative real-time polymerase chain reaction; PLA: Proximity ligation assay; IHC: Immunohistochemical staining; AOD: Average optical density. Cancer normal group: cancer adjacent tissue group. NP: No statistical difference.

Journal: World Journal of Gastroenterology

Article Title: Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1

doi: 10.3748/wjg.v28.i35.5154

Figure Lengend Snippet: Alcohol consumption increased the expression and interaction of laminin-γ2 and integrin-β1 in colorectal cancer rats. The bars represent the mean ± SD ( n = 8). A: Survival analysis of integrin-β1 (ITGB1) and laminin-γ2 (LAMC2) in colorectal cancer; B: The mRNA levels of ITGB1 and LAMC2 determined by quantitative real-time polymerase chain reaction in clinical samples; C: Representative immunohistochemical (IHC) images of ITGB1 and LAMC2 in clinical samples (× 400); D: The quantifications of the protein levels of ITGB1 and LAMC2 determined by IHC in clinical samples; E and F: Duolink proximity ligation assay (PLA) verified the interactions between ITGB1 and LAMC2 in clinical samples. The statistical analysis of PLA positive signals was performed by counting the average optical density under a light microscope. Compared with the non-alcohol group: b P < 0.01, a P < 0.05. LAMC2: Laminin-γ2; ITGB1: Integrin-β1; qRT-PCR: Quantitative real-time polymerase chain reaction; PLA: Proximity ligation assay; IHC: Immunohistochemical staining; AOD: Average optical density. Cancer normal group: cancer adjacent tissue group. NP: No statistical difference.

Article Snippet: The following antibodies were used: LAMC2 monoclonal antibody (YT5379, Immunoway, United States), ITGB1 monoclonal antibody (sc-9970, Santa Cruz, United States), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody (ab125247, ABCAM, United Kingdom), phosphorylate-FAK (p - FAK)-Y397 (AP0302, ABclonal, Wuhan, China), FAK monoclonal antibody (A11131, ABclonal, Wuhan, China), snail monoclonal antibody (ab53519, ABCAM, United Kingdom), E-cadherin polyclonal antibody (ab1416, ABCAM, United Kingdom), N-cadherin polyclonal antibody (22018-1-AP, Proteintech, United States), fibronectin polyclonal antibody (A16678, ABclonal, Wuhan, China) and special AT-rich sequence binding protein 1 (SATB1) polyclonal antibody (A5800, ABclonal, Wuhan, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunohistochemical staining, Proximity Ligation Assay, Light Microscopy, Quantitative RT-PCR, Staining

Schematic diagram of the possible signaling pathway regulated by alcohol. EMT: Epithelial mesenchymal transformation; LAMC2: Laminin-γ2; ITGB1: Integrin-β1; FAK: Focal adhesion kinase; p-FAK: Phosphorylate focal adhesion kinase.

Journal: World Journal of Gastroenterology

Article Title: Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1

doi: 10.3748/wjg.v28.i35.5154

Figure Lengend Snippet: Schematic diagram of the possible signaling pathway regulated by alcohol. EMT: Epithelial mesenchymal transformation; LAMC2: Laminin-γ2; ITGB1: Integrin-β1; FAK: Focal adhesion kinase; p-FAK: Phosphorylate focal adhesion kinase.

Article Snippet: The following antibodies were used: LAMC2 monoclonal antibody (YT5379, Immunoway, United States), ITGB1 monoclonal antibody (sc-9970, Santa Cruz, United States), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody (ab125247, ABCAM, United Kingdom), phosphorylate-FAK (p - FAK)-Y397 (AP0302, ABclonal, Wuhan, China), FAK monoclonal antibody (A11131, ABclonal, Wuhan, China), snail monoclonal antibody (ab53519, ABCAM, United Kingdom), E-cadherin polyclonal antibody (ab1416, ABCAM, United Kingdom), N-cadherin polyclonal antibody (22018-1-AP, Proteintech, United States), fibronectin polyclonal antibody (A16678, ABclonal, Wuhan, China) and special AT-rich sequence binding protein 1 (SATB1) polyclonal antibody (A5800, ABclonal, Wuhan, China).

Techniques: Transformation Assay

The UGT1A3 expression plasmid was transfected into UGT1A3-KO cells (UGT-KO), which were designated UGT-KO + UGT over, and then the LAMC2 knockdown plasmid was transfected into UGT-KO + UGT over cells, which were designated UGT-KO + UGT over+ si-LAM. A Differential expression levels of genes between UGT-KO and NC cell lines are displayed by a heat map. B Volcano plots of differentially expressed gene expression. The green or red plots are the DEGs (fold change >4 and Padj < 0.05). C DEGs were analyzed by KEGG pathway enrichment analysis. D The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO and NC cell lines. E The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO, UGT-KO + UGT over and UGT-KO + UGT over+ si-LAM cell lines. F Cell viability was detected using a CCK-8 assay (OD450 absorbance). G The colony formation ability was measured using a colony formation assay. The colonies were counted and captured. H Representative images of invasion (upper) and migration assays (lower). The number of cells was counted. Scale bar, 180 μm. I The cell cycle was measured by flow cytometry. *P < 0.05 according to Student’s t test.

Journal: Oncogene

Article Title: Identification of low-frequency variants of UGT1A3 associated with bladder cancer risk by next-generation sequencing

doi: 10.1038/s41388-021-01672-1

Figure Lengend Snippet: The UGT1A3 expression plasmid was transfected into UGT1A3-KO cells (UGT-KO), which were designated UGT-KO + UGT over, and then the LAMC2 knockdown plasmid was transfected into UGT-KO + UGT over cells, which were designated UGT-KO + UGT over+ si-LAM. A Differential expression levels of genes between UGT-KO and NC cell lines are displayed by a heat map. B Volcano plots of differentially expressed gene expression. The green or red plots are the DEGs (fold change >4 and Padj < 0.05). C DEGs were analyzed by KEGG pathway enrichment analysis. D The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO and NC cell lines. E The expression of LAMC2 at the mRNA and protein levels was detected by qRT-PCR and Western blot in the UGT-KO, UGT-KO + UGT over and UGT-KO + UGT over+ si-LAM cell lines. F Cell viability was detected using a CCK-8 assay (OD450 absorbance). G The colony formation ability was measured using a colony formation assay. The colonies were counted and captured. H Representative images of invasion (upper) and migration assays (lower). The number of cells was counted. Scale bar, 180 μm. I The cell cycle was measured by flow cytometry. *P < 0.05 according to Student’s t test.

Article Snippet: H&E staining was used to select representative areas, and IHC was applied to confirm the expression of the proliferation markers Ki67 (anti-Ki67, ab15580, Abcam), AR (anti-AR, 22089-1-AP, Proteintech), and LAMC2 (anti-LAMC2, USB-144830, US Biological).

Techniques: Expressing, Plasmid Preparation, Transfection, Knockdown, Quantitative Proteomics, Gene Expression, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay, Migration, Flow Cytometry

UGT1A3 knockout (UGT1A3-KO) or negative control (NC) cell lines were injected into humanized NCG mice. A The eight burdened NCG mice in the UGT1A3-KO and NC groups. Red arrows show the position of tumors. B The harvested tumor tissues in the UGT1A3-KO and NC groups. The mean weight of excised tumor tissues (C) and the average volume of xenografts (D) were detected in the UGT1A3-KO and NC groups. The expression of UGT1A3 (E), and LAMC2 (F) in tumors was detected using qRT-PCR. G Images of H&E staining and IHC staining for Ki67, AR, and LAMC2 expression in tumor tissues. Scale bar, 120 μm. H The graphic illustration of our findings. *P < 0.05 according to Student’s t test.

Journal: Oncogene

Article Title: Identification of low-frequency variants of UGT1A3 associated with bladder cancer risk by next-generation sequencing

doi: 10.1038/s41388-021-01672-1

Figure Lengend Snippet: UGT1A3 knockout (UGT1A3-KO) or negative control (NC) cell lines were injected into humanized NCG mice. A The eight burdened NCG mice in the UGT1A3-KO and NC groups. Red arrows show the position of tumors. B The harvested tumor tissues in the UGT1A3-KO and NC groups. The mean weight of excised tumor tissues (C) and the average volume of xenografts (D) were detected in the UGT1A3-KO and NC groups. The expression of UGT1A3 (E), and LAMC2 (F) in tumors was detected using qRT-PCR. G Images of H&E staining and IHC staining for Ki67, AR, and LAMC2 expression in tumor tissues. Scale bar, 120 μm. H The graphic illustration of our findings. *P < 0.05 according to Student’s t test.

Article Snippet: H&E staining was used to select representative areas, and IHC was applied to confirm the expression of the proliferation markers Ki67 (anti-Ki67, ab15580, Abcam), AR (anti-AR, 22089-1-AP, Proteintech), and LAMC2 (anti-LAMC2, USB-144830, US Biological).

Techniques: Knock-Out, Negative Control, Injection, Expressing, Quantitative RT-PCR, Staining, Immunohistochemistry