Journal: eLife

Article Title: Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome

doi: 10.7554/eLife.52981

Figure Lengend Snippet:

Article Snippet: Sectioned were mounted in VECTASHIELD HardSet Antifade Mounting Medium without DAPI (Vector Laboratories H-1400) and let dry overnight at 4°C.

Techniques: Labeling, Protease Inhibitor, Lysis, Plasmid Preparation, Electron Microscopy, Western Blot, Antibody Labeling, Purification, Methylation, Lambda DNA Preparation, Software

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

doi: 10.3389/fcimb.2018.00235

Figure Lengend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

Article Snippet: Herring sperm DNA (10164142), phage lambda DNA (D3779), and pBR322 (N3033L) DNA were purchased from Invitrogen (Karlsruhe, Germany), Sigma-Aldrich, and New England Biolabs (Frankfurt, Germany), respectively.

Techniques: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation

Journal: eLife

Article Title: Human RPA activates BLM’s bidirectional DNA unwinding from a nick

doi: 10.7554/eLife.54098

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Article Snippet: Recombinant DNA reagent , pTWIN1 (plasmid) , New England BioLabs , N6951S , Expression of core BLM in E. coli.

Techniques: Recombinant, Lambda DNA Preparation, Plasmid Preparation, Expressing, DNA Purification, Staining, Software

Journal: STAR Protocols

Article Title: Visualizing DNA replication by single-molecule analysis of replicated DNA

doi: 10.1016/j.xpro.2023.102721

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Article Snippet: Anti-avidin antibody, biotinylated (0.5 g /mL) (dilution 1:15) , Vector Laboratories , Cat #M0392S.

Techniques: Plasmid Preparation, Recombinant, Avidin-Biotin Assay, Marker, Lambda DNA Preparation, Saline, Agarose Gel Electrophoresis, Software, Hybridization