Journal: Journal of the American Society for Horticultural Science

Article Title: Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors

doi: 10.21273/jashs03722-16

Figure Lengend Snippet: Fig. 1. Schematic representation of the expression plasmid constructs: (A) pCAMBIA-S-SFB, (B) pCAMBIA-A-SFB, and (C) pCAMBIA-RNAi- SFB [LB = T-DNA left border, RB = T-DNA right border, SFB = S-haplotype- specific F-box protein gene, S = positive fragment, A = opposite fragment, Insertion = intermediate fragment, CaMV 35S = cauliflower mosaic virus 35S promoter, NOS-ter = nopaline synthase terminator, NPTII = neomycin phosphotransferase II gene (kanamycin resistance), GUS = b-glucuronidase gene. EcoRV, HindIII, KpnI, SalI, XbaI, SacI, and EcoRI = unique restriction endonucleases at T-DNA region].

Article Snippet: Next, the larger fragments of pBS-T and the smaller fragments of pMD19-SFB were recycled and purified using a DNA gel extraction kit (Tiangen Biochemical Technology).

Techniques: Expressing, Plasmid Preparation, Construct, Virus

Journal: Journal of the American Society for Horticultural Science

Article Title: Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors

doi: 10.21273/jashs03722-16

Figure Lengend Snippet: Fig. 3. Restriction enzyme and polymerase chain reaction (PCR) identification of pCAMBIA-S-SFB (lane M = DL 2000 DNA marker): (A) restriction pMD-SFB and pBS-T with XbaI and KpnI; restriction pMD-SFB with XbaI and KpnI (lane 1), restriction pBS-T with XbaI and KpnI (lanes 2–3); (B) restriction enzyme identification of pBS-SFB by XbaI and SalI (lanes 1–2); (C) restriction pCAMBIA and pBS-SFB with SacI and KpnIm, restriction pCAMBIA with SacI and KpnI (lane 1), restriction pBS-SFB with SacI and KpnI (lanes 2–3); (D) PCR identification of pCAMBIA-S-SFB with Saifen F1 and F2 (lanes 1–2); and (E) restriction enzyme identification of pCAMBIA-S-SFB by XbaI and SalI (lanes 1–2). SFB = S-haplotype-specific F-box gene.

Article Snippet: Next, the larger fragments of pBS-T and the smaller fragments of pMD19-SFB were recycled and purified using a DNA gel extraction kit (Tiangen Biochemical Technology).

Techniques: Polymerase Chain Reaction, Marker

Journal: Journal of the American Society for Horticultural Science

Article Title: Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors

doi: 10.21273/jashs03722-16

Figure Lengend Snippet: Fig. 2. Polymerase chain reaction amplification of S-haplotype-specific F-box (SFB) gene from ‘Xiaobaixing’ apricot (lane M = DL 2000 DNA marker): (A) specific amplification of SFB with Saifen F1 and Saifen F2 (lane 1) and (B) amplification of the 3# end of SFB gene with PFf1 and PFf2 (lanes 1–3).

Article Snippet: Next, the larger fragments of pBS-T and the smaller fragments of pMD19-SFB were recycled and purified using a DNA gel extraction kit (Tiangen Biochemical Technology).

Techniques: Polymerase Chain Reaction, Marker

Journal: Journal of the American Society for Horticultural Science

Article Title: Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors

doi: 10.21273/jashs03722-16

Figure Lengend Snippet: Fig. 6. Polymerase chain reaction (PCR) identification of expression vectors in Agrobacterium tumefaciens (lane M = DL 2000 DNA marker): (A) pCAMBIA-S-SFB in A. tumefaciens with Saifen F1 and F2 (lanes 1–4); (B) pCAMBIA-A-SFB in A. tumefaciens with Saifen F1 and F2 (lanes 1–4); and (C) pCAMBIA-RNAi-SFB in A. tumefaciens with F1 and F6 (lanes 1–4). SFB = S-haplotype-specific F-box protein gene.

Article Snippet: Next, the larger fragments of pBS-T and the smaller fragments of pMD19-SFB were recycled and purified using a DNA gel extraction kit (Tiangen Biochemical Technology).

Techniques: Polymerase Chain Reaction, Expressing, Marker

Journal: Journal of the American Society for Horticultural Science

Article Title: Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors

doi: 10.21273/jashs03722-16

Figure Lengend Snippet: Fig. 5. Construction of pCAMBIA-RNAi-SFB (lane M = DL 2000 DNA marker): (A) polymerase chain reaction (PCR) amplification of the positive fragment with primers P1 and P2 from S-haplotype-specific F-box protein gene (SFB) gene (lane 1), the opposite fragment with primers P5 and P6 from SFB (lane 2), and the intermediate fragment with primers P3 and P4 from GhHSD1 (lane 3); (B) fusion PCR products of the opposite, positive, and intermediate fragments (lane 1); (C) restriction enzyme identification of pCAMBIA-RNAi-SFB (lanes 1–3); (D) PCR identification of pCAMBIA-RNAi-SFB (lanes 1–2); and (E) schematic representation of the intron-containing hairpin RNA (ihpRNA) sequence; the whole region represents the ihpRNA sequence, whereas the highlighted fragments indicate the arm regions, the nonhighlighted region indicates the intermediate fragment from GhHSD1.

Article Snippet: Next, the larger fragments of pBS-T and the smaller fragments of pMD19-SFB were recycled and purified using a DNA gel extraction kit (Tiangen Biochemical Technology).

Techniques: Marker, Polymerase Chain Reaction, Sequencing

Journal: Journal of the American Society for Horticultural Science

Article Title: Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors

doi: 10.21273/jashs03722-16

Figure Lengend Snippet: Fig. 4. Restriction enzyme and polymerase chain reaction (PCR) identification of pCAMBIA-A-SFB: (A) restriction pCAMBIA with KpnI and SalI [lane M = Lambda DNA/EcoRI91I (BstEII) marker 15], restriction pCAMBIA with KpnI and SalI (lanes 1–2); (B) restriction pMD-SFB with KpnI and SalI [lanes 1–2 (lane M = DL 2000 DNA marker)]; (C) restriction enzyme identification of pCAMBIA-A-SFB by HindIII and EcoRI [lanes 1–2 (lane M = DL 2000 DNA marker)]; and (D) PCR identification of pCAMBIA-A-SFB with Saifen F1 and F2 [lanes 1–2 (lane M = DL 2000 DNA marker)]. SFB = S-haplotype-specific F-box protein gene.

Article Snippet: Next, the larger fragments of pBS-T and the smaller fragments of pMD19-SFB were recycled and purified using a DNA gel extraction kit (Tiangen Biochemical Technology).

Techniques: Polymerase Chain Reaction, Lambda DNA Preparation, Marker