lambda 10 3 Search Results


lambda 10  (AutoMate Scientific Inc)
93
AutoMate Scientific Inc lambda 10
Lambda 10, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda 10/product/AutoMate Scientific Inc
Average 93 stars, based on 1 article reviews
lambda 10 - by Bioz Stars, 2026-06
93/100 stars
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lambda 10 3  (Sutter Instrument Company)
93
Sutter Instrument Company lambda 10 3
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Lambda 10 3, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda 10 3/product/Sutter Instrument Company
Average 93 stars, based on 1 article reviews
lambda 10 3 - by Bioz Stars, 2026-06
93/100 stars
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lambda 10-3 shutter controller  (Sutter Instrument Company)
90
Sutter Instrument Company lambda 10-3 shutter controller
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Lambda 10 3 Shutter Controller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda 10-3 shutter controller/product/Sutter Instrument Company
Average 90 stars, based on 1 article reviews
lambda 10-3 shutter controller - by Bioz Stars, 2026-06
90/100 stars
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lambda 10-3  (SPI Supplies)
90
SPI Supplies lambda 10-3
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Lambda 10 3, supplied by SPI Supplies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda 10-3/product/SPI Supplies
Average 90 stars, based on 1 article reviews
lambda 10-3 - by Bioz Stars, 2026-06
90/100 stars
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computer-controlled filter exchanger lambda 10-3  (Sutter Instrument Company)
90
Sutter Instrument Company computer-controlled filter exchanger lambda 10-3
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Computer Controlled Filter Exchanger Lambda 10 3, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/computer-controlled filter exchanger lambda 10-3/product/Sutter Instrument Company
Average 90 stars, based on 1 article reviews
computer-controlled filter exchanger lambda 10-3 - by Bioz Stars, 2026-06
90/100 stars
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lambda 10-3 controller  (Sutter Instrument)
90
Sutter Instrument lambda 10-3 controller
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Lambda 10 3 Controller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda 10-3 controller/product/Sutter Instrument
Average 90 stars, based on 1 article reviews
lambda 10-3 controller - by Bioz Stars, 2026-06
90/100 stars
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sutter lambda 10-3 lter wheels  (Sutter Instrument Company)
90
Sutter Instrument Company sutter lambda 10-3 lter wheels
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Sutter Lambda 10 3 Lter Wheels, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sutter lambda 10-3 lter wheels/product/Sutter Instrument Company
Average 90 stars, based on 1 article reviews
sutter lambda 10-3 lter wheels - by Bioz Stars, 2026-06
90/100 stars
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fluorescence filter exchanger lambda 10-3  (Sutter Instrument Company)
90
Sutter Instrument Company fluorescence filter exchanger lambda 10-3
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Fluorescence Filter Exchanger Lambda 10 3, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda 10-3  (Sutter Instrument)
90
Sutter Instrument lambda 10-3
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Lambda 10 3, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lm 103  (Lambda Physik USA Inc)
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Lambda Physik USA Inc lm 103
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Lm 103, supplied by Lambda Physik USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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filter wheel system sutter lambda 10-3 with chroma 340 and excitation filters  (Sutter Instrument Company)
90
Sutter Instrument Company filter wheel system sutter lambda 10-3 with chroma 340 and excitation filters
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Filter Wheel System Sutter Lambda 10 3 With Chroma 340 And Excitation Filters, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neutral density filters lambda 10-2  (Sutter Instrument Company)
90
Sutter Instrument Company neutral density filters lambda 10-2
(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.
Neutral Density Filters Lambda 10 2, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.

Journal: PLoS Biology

Article Title: Distinct Roles for Arp2/3 Regulators in Actin Assembly and Endocytosis

doi: 10.1371/journal.pbio.0060001

Figure Lengend Snippet: (A–G) Null mutants lacking one or both type-I myosin. Cells express Sla2-GFP (A–D) or Abp1-GFP (E–G). (A) MSD plots for wild-type and myo5 Δ patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (D) Phase II movement only. For each patch, data prior to movement were removed. (E) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. On the left, plots are truncated at the median lifetime or 16 s, whichever was less. (F) Percentage of patches that leave the origin, as in (B). (G) Average time at the origin, as in (C). (H–M) Mutants lacking Arp2/3-binding regions of one or both type-I myosins, termed myo3 Δ acidic and myo5 Δ acidic . The panels are similar to those above, with cells expressing Sla2-GFP (H–J) or Abp1-GFP (K–M). (H and K) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (I and L) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (J and M) Average time at the origin, from the appearance of a patch until it moved away or disappeared. (N–S) Mutants lacking Arp2/3 binding regions of WASp/Las17 and type-I myosins. Cells express Sla2-GFP (N–P) or Abp1-GFP (Q–S). (N and Q) MSD plots for wild-type or mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (O and P) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (R and S) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and number of patches: (A–C) Wild-type, YJC4787–9; 94, 79, and 90. myo5 Δ, YJC4784–6; 103, 79, and 69. (D) Strains as in (A–C). Numbers of patches: wild-type – 88, 69, 85. myo5 Δ – 75, 40, 36. (E–G) Wild-type, YJC4815–7; 121, 110, and 130. myo3 Δ, YJC4409–11; 111, 115, and 105. myo5 Δ, YJC4813–4; 120 and 99. myo3 Δ myo5 Δ, YJC4770–2; 143, 141, and 126. (H–J) Wild-type, YJC5566–8; 71, 107, and 83. myo3 Δ acidic , YJC5563–5; 71, 75, and 101. myo5 Δ acidic , YJC5554–6; 86, 71, and 62. myo3 Δ acidic myo5 Δ acidic , YJC5557–9; 83, 65, and 83. (K–M) Wild-type, YJC4848 and 50; 116 and 87. myo3 Δ acidic , YJC4843–5; 119, 99, and 106. myo5 Δ acidic , YJC4840–2; 102, 111, and 99. myo3 Δ acidic myo5 Δ acidic , YJC4837–9; 96, 93, and 100. (N–P) Wild-type, YJC5566–8; 71, 107, and 83. las17 Δ acidic , YJC5551–3; 84, 64, and 69. las17 Δ acidic myo3 Δ acidic , YJC5560–2; 89, 71, and 68. las17 Δ acidic myo5 Δ acidic , YJC5548–50; 51, 66, and 57. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5545–7; 43, 53, and 54. (Q–S) las17 Δ acidic , YJC5208–10; 95, 76, and 89. las17 Δ acidic myo3 Δ acidic , YJC5438–40; 111, 104, and 109. las17 Δ acidic myo5 Δ acidic , YJC5441–3; 98, 80, and 84. las17 Δ acidic myo3 Δ acidic myo5 Δ acidic , YJC5205–7; 71, 79, and 99.

Article Snippet: Two color images were collected sequentially using a LMM5 Laser Merge Module (Spectral Applied Research), a multipass dichroic mirror, and emission filters in a Lambda 10–3 high-speed filter wheel (Sutter Instruments).

Techniques: Mutagenesis, Binding Assay, Expressing

Cells express Sla2-GFP (A–C) or Abp1-GFP (D–F). ( A and D) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B and E) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C and F) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and numbers of patches: (A–C) Wild-type, YJC5707 - 9; 66, 84, and 69. las17 Δ acidic , YJC5713–5; 100, 95, and 104. pan1 Δ acidic , YJC5710–2; 103, 76, and 74. las17 Δ acidic pan1 Δ acidic , YJC5716–8; 100, 86, and 91. (D–F) Wild-type, YJC5719–22; 99, 100, 114, and 98. las17 Δ acidic , YJC5726, 8 & 9; 95, 88, and 84. pan1 Δ acidic , YJC5723–5; 102, 94, and 139. las17 Δ acidic pan1 Δ acidic , YJC5730–3; 77, 70, 50, and 50. Error bars are ± standard error.

Journal: PLoS Biology

Article Title: Distinct Roles for Arp2/3 Regulators in Actin Assembly and Endocytosis

doi: 10.1371/journal.pbio.0060001

Figure Lengend Snippet: Cells express Sla2-GFP (A–C) or Abp1-GFP (D–F). ( A and D) MSD plots for wild-type and mutant patches aligned at the start (left) or end (right) of their lifetimes. The curves on the left are truncated at the median lifetime. (B and E) Percentage of patches that leave the origin. Mean of values for three segregants is shown. (C and F) Average time at the origin, from the appearance of a patch until it moved away or disappeared. Strain numbers and numbers of patches: (A–C) Wild-type, YJC5707 - 9; 66, 84, and 69. las17 Δ acidic , YJC5713–5; 100, 95, and 104. pan1 Δ acidic , YJC5710–2; 103, 76, and 74. las17 Δ acidic pan1 Δ acidic , YJC5716–8; 100, 86, and 91. (D–F) Wild-type, YJC5719–22; 99, 100, 114, and 98. las17 Δ acidic , YJC5726, 8 & 9; 95, 88, and 84. pan1 Δ acidic , YJC5723–5; 102, 94, and 139. las17 Δ acidic pan1 Δ acidic , YJC5730–3; 77, 70, 50, and 50. Error bars are ± standard error.

Article Snippet: Two color images were collected sequentially using a LMM5 Laser Merge Module (Spectral Applied Research), a multipass dichroic mirror, and emission filters in a Lambda 10–3 high-speed filter wheel (Sutter Instruments).

Techniques: Mutagenesis