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Image Search Results
Journal: The EMBO Journal
Article Title: RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity
doi: 10.15252/embj.201488979
Figure Lengend Snippet: A–E Mitotic control U2OS cells or U2OS cells treated with RBM14 siRNA were stained with antibodies against centrin-2 (green) and C-Nap1 (magenta) (A), Centrobin (green) and C-Nap1 (magenta) (B), centrin-2 (green) and CPAP (magenta) (C), acetylated-tubulin (green) and CP110 (magenta) (D), and centrin-2 (green) and γ-tubulin (magenta) (E). DNA is shown in blue. Insets show approximately twofold magnified views of fluorescent foci around the centrosome. Scale bar, 5 μm. Histograms on the right represent frequency of mitotic cells with excess foci of the indicated proteins at spindle poles in each condition. Values are mean percentages ± standard error of mean (SEM) from three independent experiments ( n = 30 for each condition). * P < 0.05, ** P < 0.01, n.s., not significant (one-tailed t -test). Note that we counted the number of C-Nap1 foci after anaphase in mitosis when C-Nap1 signals recover from the reduction in prometaphase.
Article Snippet: The following primary antibodies were used in this study: rabbit polyclonal antibodies against STIL (Abcam, ab89314, IF 1:1,000, WB 1:1,000), RBM14 (Abcam, ab12325, IF 1:300, WB 1:1,000), SAS-6 (Santa Cruz Biotechnology, Inc., sc-98506, IF 1:300, WB 1:1,000), Cep135 (Abcam, ab75005, IF 1:300), Cep192 (a gift from Laurence Pelletier, IF 1:1,000), Cep152 (Bethyl laboratories, A302-480A, IF 1:1,000), CP110 (a gift from Brian David Dynlacht, IF 1:500),
Techniques: Control, Staining, One-tailed Test
Journal: Frontiers in Immunology
Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging
doi: 10.3389/fimmu.2024.1383932
Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.
Article Snippet: LAG3 ,
Techniques: Imaging