Journal: Molecular Biology of the Cell

Article Title: Growth disadvantage associated with centrosome amplification drives population-level centriole number homeostasis

doi: 10.1091/mbc.e19-04-0195

Figure Lengend Snippet: Figure 5. PLK4 and p53 relationship in cancers and summary of findings. (A) PLK4 expression in human cancers. Data are represented as beeswarm plots showing mRNA expression levels and organized form left to right in order of increasing median PLK4 expression; medians are denoted by orange lines. Transcriptomic and genomic data, obtained from the TCGA Research Network PanCanAtlas and visualized with cBioPortal, are RNA-seq data from Illumina HiSeq_RNASeqV2. Percentages of cancers with altered p53 pathway transcripts (TP53, CDKN1A, CDKN2A, MDM2, MDM4, and RB1) are also from the PanCanAtlas and includes mutation, fusion, amplification, deep deletion, and multiple alterations (see also Figure S4). (B) log2(PLK4 mRNA) in testicular germ cell tumors with or without genomic and transcriptomic alterations to TP53. Transcriptomic and genomic data, obtained from the TCGA Research Network PanCanAtlas, are RNA-seq data from Illumina HiSeq_RNASeqV2. (C) Comparison between calculated population growth (theoretical) and experimental (empirical) data. The model considers 10% of normal cells (2-4 centrioles) in the starting population. In the equations, N(t) represents the population size of normal (n) cells or ca (centriole amplified) cells; represents the proportion of cells that divide; represents the rate of mitoses per day; represents the death rate in the population. See Experimental procedures for details. (D) Schematic to summarize fates of cells with centriole amplification.

Article Snippet: For stabilization of p53 treatment, 10 M Nutlin3a (Selleckchem) was added to cells 48 h before lysis in 1x Laemlli buffer.

Techniques: Expressing, RNA Sequencing, Mutagenesis, Amplification, Comparison