Journal: Nature

Article Title: Functional identification of an aggression locus in the mouse hypothalamus

doi: 10.1038/nature09736

Figure Lengend Snippet: a, Anti-GFP antibody staining (green) in mice bilaterally infected with AAV2-GluClα + AAV2-GluClβ. Scale bar: 500 μm. b-e, Overlap between GluCl-expressing (green) and Fos-expressing (red) cells, 1hr after fighting. Blue: Topro-3 nuclear stain. Inset in ( c ) represents boxed area. Yellow cells are double-labeled. Scale bars: 50 μm or 10 μm (inset). f, g, Percent change in cumulative attack duration ( f ) and latency ( g ) during a 600 s resident-intruder trial before vs. 24 hr after IVM injection. Test: GluCl virus-injected animals (n = 33) (red bar). Control: no surgery (white bar, n = 12), saline (black bar, n = 6) or GluClß virus-injected animals (n = 12, gray bar) (**, P < 0.01, *, P < 0.05, t-test). h , Cumulative attack duration during repeated cycles of IVM injection or washout (*, P < 0.05, Bonferroni posttests of two-way ANOVA with repeated measures). Test: GluCl virus-injected animals (n = 12); Control: Saline (n = 6). i, j, Percent change in mount duration ( i ) or latency ( j ) in test (n = 12) vs. control (GluClß virus injected, n = 12) males paired with females. k . Percentage of infected cells in posterior portion of VMHvl (Bregma −1.4 mm — 1.8 mm) plotted against extent of aggression suppression after IVM injection. The Pearson correlation coefficient is significantly higher than 0 ( P < 0.001). See for further analysis.

Article Snippet: AAV2 CMV::CRE and AAV2 CMV::LacZ viruses were purchased from Vectorbiolabs.

Techniques: Staining, Infection, Expressing, Labeling, Injection, Virus, Control, Saline

Journal:

Article Title: Analysis of dofA , a fruA -Dependent Developmental Gene, and Its Homologue, dofB , in Myxococcus xanthus

doi: 10.1128/JB.184.24.6803-6810.2002

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: pGEM-T easy , Ap r lacZ cloning vector , Promega.

Techniques: Plasmid Preparation, Clone Assay

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Angiopoietin-1 prevents severe bleeding complications induced by heparin-like drugs and fibroblast growth factor-2 in mice

doi: 10.1152/ajpheart.00373.2015

Figure Lengend Snippet: PPS did not affect the expression of metalloproteinases or VEGF-A in the intestine of mice infected with rAd-LacZ. A: representative pictures of the Western blots done with samples derived from the small intestinal of mice infected with rAd-LacZ vectors and treated with PPS (60 mg/kg) or phosphate-buffered saline (PBS). Only the intestinal expression of ICAM-1 was significantly increased in mice treated with rAd-LacZ + PPS relative to the control mice infected with rAd-LacZ (*P < 0.05; n = 6 samples per group). B: the densitometric analysis corresponding to the Western blots. Results are expressed in arbitrary optical density units as ratio of the beta-actin expression (means ± SE). Samples from 6 different mice were included in each group.

Article Snippet: Recombinant adenoviral (rAd) vectors. rAd-vectors carrying either the Escherichia coli LacZ gene (rAd-Lac-Z), a 700-bp cDNA sequence encoding a secreted form of human recombinant FGF-2 (rAd-FGF-2), or a genetically modified form of angiopoietin-1 (Ang-1*) from Regeneron Pharm (Tarrytown, NY), were generated, amplified, and purified as previously described ( 13 , 21 , 44 , 50 ).

Techniques: Expressing, Infection, Western Blot, Derivative Assay, Saline, Control