Journal: Cell Biology and Toxicology

Article Title: Chronic cadmium exposure promotes TRPM7-dependent acquisition of a myofibroblast-like phenotype in pancreatic stellate cells

doi: 10.1007/s10565-026-10169-0

Figure Lengend Snippet: Expression of pancreatic stellate cell markers in PS-1 cells chronically exposed to Cd. A Protein level expression of α-SMA studied by western-blot ( n = 6). B Protein level expression of vimentin studied by western-blot ( n = 7). C Protein level expression of desmin studied by western-blot ( n = 7). D Protein level expression of GFAP studied by western-blot ( n = 5). E Protein level expression of α-SMA studied by immunofluorescence ( n = 3). F Protein level expression of vimentin studied by immunofluorescence ( n = 3). G Protein level expression of desmin studied by immunofluorescence ( n = 3). H. Protein level expression of GFAP studied by immunofluorescence ( n = 3). Bar = 10 µm. * p < 0.05, unpaired Student t-tests

Article Snippet: PS-1 cells were seeded onto Labtek® chambers and allowed to adhere overnight.

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: Cell Biology and Toxicology

Article Title: Chronic cadmium exposure promotes TRPM7-dependent acquisition of a myofibroblast-like phenotype in pancreatic stellate cells

doi: 10.1007/s10565-026-10169-0

Figure Lengend Snippet: Expression of matrix extracellular proteins and inflammatory cytokines in PS-1 cells chronically exposed to Cd. A Protein level expression of type I collagen studied by western-blot ( n = 8). B Protein level expression of elastin studied by western-blot ( n = 11). C Protein level expression of fibronectin studied by western-blot ( n = 8). D Protein level expression of type I collagen studied by immunofluorescence ( n = 3). E Protein level expression of elastin studied by immunofluorescence ( n = 3). F Protein level expression of fibronectin studied by immunofluorescence ( n = 3). G Expression of secreted IL-8 ( n = 3). H Expression of secreted IL-10 by ELISA assays ( n = 3). Bar = 100 µm. ** p < 0.01; *** p < 0.001, Mann–Whitney (B, H) or unpaired Student t-tests (A, C, G)

Article Snippet: PS-1 cells were seeded onto Labtek® chambers and allowed to adhere overnight.

Techniques: Expressing, Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Cell Biology and Toxicology

Article Title: Chronic cadmium exposure promotes TRPM7-dependent acquisition of a myofibroblast-like phenotype in pancreatic stellate cells

doi: 10.1007/s10565-026-10169-0

Figure Lengend Snippet: Effect of Cd exposure on PS-1 cell migration. A MMP-2 secretion in control and Cd exposed PS-1 ( n = 4). B uPA secretion in control and Cd exposed PS-1 ( n = 4). C Cell migration of control and Cd exposed PS-1 cells assessed by wound healing assay. Photographs were taken at magnification × 50 right after the wound (T0) and 24 h after (T24) ( n = 4) D Cell migration of control and Cd exposed PS-1 cells evaluated in Boyden chambers ( n = 6). Bar = 10 µm. *** p < 0.001, Mann–Whitney tests

Article Snippet: PS-1 cells were seeded onto Labtek® chambers and allowed to adhere overnight.

Techniques: Migration, Control, Wound Healing Assay, MANN-WHITNEY

Journal: Cell Biology and Toxicology

Article Title: Chronic cadmium exposure promotes TRPM7-dependent acquisition of a myofibroblast-like phenotype in pancreatic stellate cells

doi: 10.1007/s10565-026-10169-0

Figure Lengend Snippet: Role of TRPM7 in PS-1 cell exposed to cadmium. A Protein level expression of TRPM7 studied by western-blot ( n = 5). B Averaged current–voltage relationship of MIC currents recorded between −100 and + 100 mV (left) and current-densities measured at + 100 mV in control and Cd exposed PS-1 cells (right; n = 5). C Effect of TRPM7 silencing on TRPM7 mRNA expression ( n = 6). D Averaged current–voltage relationship of MIC currents recorded between −100 and + 100 mV (left) and current-densities measured at + 100 mV in control and Cd exposed PS-1 cells transfected with siTRPM7 or scrambled siRNA (siCTL) (right; n = 4 for control cells; n = 3 for Cd exposed cells). E Representative photographs of migrating cells through Boyden chambers exposed to the different experimental conditions. F Effect of Cd exposure on control ( n = 3) and TRPM7 silenced ( n = 3) cell migration. G Effect of Cd exposure on control ( n = 5) and NS8593 treated ( n = 5) cell migration. Bar = 10 µm. * p < 0.05; ** p < 0.01; *** p < 0.001, Mann–Whitney (A) or unpaired Student t-tests (B), or 2-ways ANOVA followed by Šídák's multiple comparisons test (C, D, F, G)

Article Snippet: PS-1 cells were seeded onto Labtek® chambers and allowed to adhere overnight.

Techniques: Expressing, Western Blot, Control, Transfection, Migration, MANN-WHITNEY

Journal: Cell Biology and Toxicology

Article Title: Chronic cadmium exposure promotes TRPM7-dependent acquisition of a myofibroblast-like phenotype in pancreatic stellate cells

doi: 10.1007/s10565-026-10169-0

Figure Lengend Snippet: Effect of Cd exposure on the interaction between pancreatic stellate cells and cancer cells. A Schematic representation of the co-culture protocol (top panel) and representative photographs of migrating MIA PaCa-2 through Boyden chambers (low panel). B Measurement of MIA PaCa-2 migration stimulated by PS-1 exposed to Cd ( n = 5). C Schematic representation of the co-culture protocol (top panel) and representative photographs of migrating MIA PaCa-2 through Boyden chambers (low panel). D Effect of NS8593 on MIA PaCa-2 cell migration stimulated by PS-1 cells ( n = 3). E Schematic representation of the co-culture protocol (top panel) and representative photographs of migrating MIA PaCa-2 through Boyden chambers (low panel). F Effect of TRPM7 silencing on MIA PaCa-2 cell migration stimulated by PS-1 cells ( n = 6). Bar = 10 µm. * p < 0.05; *** p < 0.001, ANOVA followed by Tukey's multiple comparisons test (B) or 2-ways ANOVA followed by Šídák's multiple comparisons test (D and F)

Article Snippet: PS-1 cells were seeded onto Labtek® chambers and allowed to adhere overnight.

Techniques: Co-Culture Assay, Migration