Journal: Molecular Medicine Reports

Article Title: Heat shock protein 72 suppresses apoptosis by increasing the stability of X-linked inhibitor of apoptosis protein in renal ischemia/reperfusion injury

doi: 10.3892/mmr.2014.2939

Figure Lengend Snippet: Effects of GGA on renal tubular cell apoptosis. (A) Representative images of TUNEL in different groups as indicated (magnification, ×400). (B) Morphometric analysis of the number of TUNEL-positive cells per high-power field in microscopy. (C) Expression of XIAP and HSP72 in the kidney was examined by immunoblot, β-actin served as a loading control. (D) Graphic representation of XIAP protein levels in different groups, as indicated following normalization with β-actin content. Data in B and D are expressed as the mean ± standard error of the mean; * P<0.01 versus sham-surgery rats; # P<0.05 versus vehicle-treated rats. GGA, geranylgeranylacetone; XIAP, X-linked inhibitor of apoptosis protein; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; HSP72, heat shock protein 72.

Article Snippet: Apoptosis was quantified in histological sections using a commercially available TACS TdT-Fluor In Situ Apoptosis Detection kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and our previous study ( ).

Techniques: TUNEL Assay, Microscopy, Expressing, Western Blot, End Labeling

Journal: Molecular Medicine Reports

Article Title: Heat shock protein 72 suppresses apoptosis by increasing the stability of X-linked inhibitor of apoptosis protein in renal ischemia/reperfusion injury

doi: 10.3892/mmr.2014.2939

Figure Lengend Snippet: Effect of HSP72 on the expression of apoptotic proteins. (A) XIAP and pro-caspase 3 were assessed by immunoblot following overexpressing HSP72. Empty vector served as negative control; (B) Quantitative determination of the relative abundance of XIAP among different groups. (C) Quantitative determination of the relative abundance of pro-caspase 3 among different groups. Data in B and C are expressed as the mean ± standard error of the mean of three experiments. * P<0.01 versus empty vector control. XIAP, X-linked inhibitor of apoptosis protein; ATP, adenosine triphosphate; HSP72, heat shock protein 72.

Article Snippet: Apoptosis was quantified in histological sections using a commercially available TACS TdT-Fluor In Situ Apoptosis Detection kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and our previous study ( ).

Techniques: Expressing, Western Blot, Plasmid Preparation, Negative Control

Journal: Molecular Medicine Reports

Article Title: Heat shock protein 72 suppresses apoptosis by increasing the stability of X-linked inhibitor of apoptosis protein in renal ischemia/reperfusion injury

doi: 10.3892/mmr.2014.2939

Figure Lengend Snippet: Interaction between HSP72 and XIAP. XIAP was immunoprecipitated from whole Human kidney-2 cell lysates among different groups using a rabbit polyclonal anti-XIAP antibody. HSP72 content was assessed using an anti-HSP72 antibody (upper panel). Immunoblot analysis was used to localize XIAP in cell lysates (lower panel). XIAP, X-linked inhibitor of apoptosis protein; ATP, adenosine triphosphate; IP, immunoprecipitation; IB, immunoblotting; HSP72, heat shock protein 72.

Article Snippet: Apoptosis was quantified in histological sections using a commercially available TACS TdT-Fluor In Situ Apoptosis Detection kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions and our previous study ( ).

Techniques: Immunoprecipitation, Western Blot