l6 line Search Results


l6 cell line overexpressing glut4 with a myc protein tag (glut4-myc)  (AstraZeneca ltd)
90
AstraZeneca ltd l6 cell line overexpressing glut4 with a myc protein tag (glut4-myc)
L6 Cell Line Overexpressing Glut4 With A Myc Protein Tag (Glut4 Myc), supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/l6+cell+line+overexpressing+glut4+with+a+myc+protein+tag++glut4+myc+/pmc04187550-83-27-17
Average 90 stars, based on 1 article reviews
l6 cell line overexpressing glut4 with a myc protein tag (glut4-myc) - by Bioz Stars, 2026-09
90/100 stars
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l6 skeletal muscle cell line  (National Centre for Cell Science)
90
National Centre for Cell Science l6 skeletal muscle cell line
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
L6 Skeletal Muscle Cell Line, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/l6+skeletal+muscle+cell+line/pmc05224995-70-0-13
Average 90 stars, based on 1 article reviews
l6 skeletal muscle cell line - by Bioz Stars, 2026-09
90/100 stars
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l6 cell line  (National Centre for Cell Science)
90
National Centre for Cell Science l6 cell line
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
L6 Cell Line, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/l6+cell+line/10__1186_slash_s43094___024___00736___9-66-1-8
Average 90 stars, based on 1 article reviews
l6 cell line - by Bioz Stars, 2026-09
90/100 stars
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rat l6 cell line  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures rat l6 cell line
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
Rat L6 Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/rat+l6+cell+line/pm39876762-97-7-15
Average 90 stars, based on 1 article reviews
rat l6 cell line - by Bioz Stars, 2026-09
90/100 stars
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l6 rat myoblast cell line  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare l6 rat myoblast cell line
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
L6 Rat Myoblast Cell Line, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/l6+rat+myoblast+cell+line/pmc06503959-336-1-12
Average 90 stars, based on 1 article reviews
l6 rat myoblast cell line - by Bioz Stars, 2026-09
90/100 stars
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l6  (ATCC)
96
ATCC l6
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
L6, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/L6/custom%40crl-1458%4029513566
Average 96 stars, based on 1 article reviews
l6 - by Bioz Stars, 2026-09
96/100 stars
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t-cell line specific for the l6/6 id  (ACADEMIC PRESS INC)
90
ACADEMIC PRESS INC t-cell line specific for the l6/6 id
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
T Cell Line Specific For The L6/6 Id, supplied by ACADEMIC PRESS INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/t+cell+line+specific+for+the+l6+6+id/pm01830833-21-45-7
Average 90 stars, based on 1 article reviews
t-cell line specific for the l6/6 id - by Bioz Stars, 2026-09
90/100 stars
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l-6 cell line  (Tibotec Pharmaceuticals)
90
Tibotec Pharmaceuticals l-6 cell line
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
L 6 Cell Line, supplied by Tibotec Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/l+6+cell+line/pm29550531-25-47-7
Average 90 stars, based on 1 article reviews
l-6 cell line - by Bioz Stars, 2026-09
90/100 stars
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low-body-weight selection line l6  (Rocha labs)
90
Rocha labs low-body-weight selection line l6
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
Low Body Weight Selection Line L6, supplied by Rocha labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/low+body+weight+selection+line+l6/pm15672592-18-12-32
Average 90 stars, based on 1 article reviews
low-body-weight selection line l6 - by Bioz Stars, 2026-09
90/100 stars
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l6 cell line  (JCRB Cell Bank)
90
JCRB Cell Bank l6 cell line
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
L6 Cell Line, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/l6+cell+line/pm31158782-54-7-20
Average 90 stars, based on 1 article reviews
l6 cell line - by Bioz Stars, 2026-09
90/100 stars
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rat skeletal muscle myoblast cell line l6.c11  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures rat skeletal muscle myoblast cell line l6.c11
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
Rat Skeletal Muscle Myoblast Cell Line L6.C11, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+line/rat+skeletal+muscle+myoblast+cell+line+l6+c11/pm31159662-37-1-9
Average 90 stars, based on 1 article reviews
rat skeletal muscle myoblast cell line l6.c11 - by Bioz Stars, 2026-09
90/100 stars
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l6 rat skeletal muscle myoblast cell line  (Pasteur Institute)
86
Pasteur Institute l6 rat skeletal muscle myoblast cell line
(A) dmp fails to activate EGFR. <t>L6</t> myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes <t>or</t> <t>skeletal</t> muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).
L6 Rat Skeletal Muscle Myoblast Cell Line, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) dmp fails to activate EGFR. L6 myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes or skeletal muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).

Journal: PLoS ONE

Article Title: A Small Insulinomimetic Molecule Also Improves Insulin Sensitivity in Diabetic Mice

doi: 10.1371/journal.pone.0169809

Figure Lengend Snippet: (A) dmp fails to activate EGFR. L6 myotubes or 3T3L1 adipocytes were treated with or without 250 nM dmp for 4h. The cell lysates were analyzed by immunoblotting with anti-pEGFR and anti-EGFR antibodies. (B,C) dmp can induce IR phosphorylation in a dose dependent manner. L6 myotubes were treated with insulin (20–120 n m ) or dmp (50–300 n m ) for 4h and IR phosphorylation was monitored by ELISA (B) or immunoblotting with anti-pIR and anti-IR antibodies (C). (D) IR kinase activity was determined in L6 myotubes which were incubated with varied concentrations of insulin or dmp. (E) dmp stimulates IR and its downstream kinases phosphorylation. L6 myotubes or 3T3L1 adipocytes were treated with or without Insulin (100 nM) or dmp (250 nM) for 4h and the IR phosphorylation and its downstream signalling were monitored by immunoblotting. (F) L6 myotubes transfected with GFP-GLUT4 chimeric gene were incubated with insulin (100 nM) or dmp (250nM) for 4h. Cells on the cover slips were fixed in paraformaldehyde and observed under florescent microscope for GFP-GLUT4 translocation. (G) dmp like insulin promotes glucose uptake. L6 myotubes or skeletal muscle cells from soleus muscle of neonatal mice (2-3days) were incubated with 100 n m insulin or 250 n m dmp for 25 min. [ 14 C] 2-DOG was then added, and the cells were further incubated for 5 min. [ 14 C] 2-DOG uptake was measured by scintillation counting. * P <0.05 versus Con; ** P <0.01 versus Con. (H) dmp augments fatty acid uptake. Primary culture adipocytes or 3T3L1 adipocytes were treated with 100 n m insulin or 250 n m dmp for 4h followed by incubation with [ 3 H] Palmitate for 15 min. [ 3 H] Palmitate uptake was measured in a liquid scintillation counter. * P <0.05 versus Con; ** P <0.01 versus Con. (I) L6 myotubes were tranfected with IR siRNA(IR KD ) followed by estimation of IR gene and protein levels by qPCR (left) and immunoblotting (right) respectively. * P <0.05 versus Con. (J) IR KD L6 myotubes were incubated with dmp for 4h and [ 14 C] 2-DOG uptake was measured according to the above description. ** P <0.01 versus Con. All values are represented as mean ± s.e.m. (n = 5).

Article Snippet: L6 skeletal muscle cell line and mouse pre-adipocyte 3T3L1 were procured from the National Centre for Cell Science, Pune, India.

Techniques: Western Blot, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Activity Assay, Incubation, Transfection, Microscopy, Translocation Assay

Rats were fed with dmp (5 mg kg bw -1 ) for 14 days. (A) Hematological, (B) Biochemical analysis, (C) Liver and kidney histopathology were performed on 0 and 14 day to test toxic effect of dmp. (D) L6 myotubes and 3T3L1 adipocytes were incubated with 300 nM of dmp for 4 h and cell viability was assessed by MTT assay. (E) After incubation of 3T3L1 adipocytes with or without 300 nM dmp for 4 h, release of TNFα and IL6 in to the medium was estimated through ELISA. (F) ATP production was measured in L6 myotubes incubated in the absence or presence of 300nM dmp for 4 h. All values are represented as mean ± s.e.m. (n = 5)

Journal: PLoS ONE

Article Title: A Small Insulinomimetic Molecule Also Improves Insulin Sensitivity in Diabetic Mice

doi: 10.1371/journal.pone.0169809

Figure Lengend Snippet: Rats were fed with dmp (5 mg kg bw -1 ) for 14 days. (A) Hematological, (B) Biochemical analysis, (C) Liver and kidney histopathology were performed on 0 and 14 day to test toxic effect of dmp. (D) L6 myotubes and 3T3L1 adipocytes were incubated with 300 nM of dmp for 4 h and cell viability was assessed by MTT assay. (E) After incubation of 3T3L1 adipocytes with or without 300 nM dmp for 4 h, release of TNFα and IL6 in to the medium was estimated through ELISA. (F) ATP production was measured in L6 myotubes incubated in the absence or presence of 300nM dmp for 4 h. All values are represented as mean ± s.e.m. (n = 5)

Article Snippet: L6 skeletal muscle cell line and mouse pre-adipocyte 3T3L1 were procured from the National Centre for Cell Science, Pune, India.

Techniques: Histopathology, Incubation, MTT Assay, Enzyme-linked Immunosorbent Assay