Journal: Scientific Reports

Article Title: The iodide transporter Slc26a7 impacts thyroid function more strongly than Slc26a4 in mice

doi: 10.1038/s41598-022-15151-4

Figure Lengend Snippet: Serological phenotypes of the deficient mice. ( a ) Serum free thyroxine (FT4), free triiodothyronine (FT3), and thyrotropin (TSH) levels in male wild type (WT), Slc26a7 +/− , and Slc26a7 −/− mice at day 45; n = 4–7 mice of each genotype. One-way analysis of variance (ANOVA) showed significant differences in FT4 and TSH levels (FT4: F (2, 11) = 23.4, p = 0.0001; TSH: F (2, 10) = 22.5, p = 0.0002), but not in FT3 levels ( F (2, 10) = 1.76, p = 0.219). *** p < 0.001 determined by Tukey’s test. ( b ) Serum FT4, FT3, and TSH levels in male WT, Slc26a4 −/− , Slc26a7 −/− , and Slc26a7 del/del Slc26a4 −/− mice at day 90; n = 4–6 mice of each genotype. One-way ANOVA showed significant differences in FT4, FT3, and TSH levels (FT4: F (3, 16) = 22.5, p < 0.0001; FT3: F (3, 14) = 5.29, p = 0.120; TSH: F (3, 14) = 30.3, p < 0.0001). * p < 0.05, *** p < 0.001, **** p < 0.0001 determined by Tukey’s test. FT4 free thyroxine, FT3 free triiodothyronine, TSH thyrotropin, WT wild type.

Article Snippet: Thyroid hormone replacement with albuminized drinking water containing 0.39 mg/L l -thyroxine (L-T4) (L-T4 sodium salt pentahydrate; Med Chem Express, NJ) was started after weaning on day 21.

Techniques:

Journal: Scientific Reports

Article Title: The iodide transporter Slc26a7 impacts thyroid function more strongly than Slc26a4 in mice

doi: 10.1038/s41598-022-15151-4

Figure Lengend Snippet: Phenotypes of Slc26a7 −/− and Slc26a4 −/− mice on a low iodine diet. ( a ) Urine iodine levels of female Slc26a7 +/− mice with normal and low iodine diets; n = 6 mice in each group. ** p < 0.01 determined by Student’s t test. ( b ) Growth curve of male wild type (WT), Slc26a7 +/− , Slc26a7 −/− , and Slc26a7 −/− mice receiving l -thyroxine (L-T4) replacement from day 21 with a low iodine diet. Note that Slc26a7 −/− mice without thyroid hormone replacement did not survive. ( c ) Serum free thyroxine (FT4) levels in male WT, Slc26a7 +/− , and Slc26a7 −/− mice with normal and low iodine diet at day 21; n = 4–5 mice of each genotype. Two-way analysis of variance (ANOVA) showed significant effects of genotype ( F (2, 20) = 29.92, p < 0.0001). ** p < 0.01, *** p < 0.001 determined by Tukey’s test compared with WT mice with same diet. ( d ) Serum FT4 levels in male WT, Slc26a4 +/− , and Slc26a4 −/− mice that were fed a low iodine diet, at day 90; n = 4–5 mice of each genotype. One-way ANOVA showed no significant differences among the three groups ( F (2, 10) = 0.19, p = 0.83). WT wild type, L-T4 l -thyroxine, FT4 free thyroxine.

Article Snippet: Thyroid hormone replacement with albuminized drinking water containing 0.39 mg/L l -thyroxine (L-T4) (L-T4 sodium salt pentahydrate; Med Chem Express, NJ) was started after weaning on day 21.

Techniques:

Journal: Scientific Reports

Article Title: The iodide transporter Slc26a7 impacts thyroid function more strongly than Slc26a4 in mice

doi: 10.1038/s41598-022-15151-4

Figure Lengend Snippet: Gene expression profiles were greatly altered in Slc26a7 −/− mice. ( a , b ) Global patterns of gene expression in wild type (WT) (male n = 4), Slc26a7 −/− (male n = 4), and Slc26a4 −/− (male n = 3) mice are depicted using a MA plot with counts per million (CPM) and fold change (FC). Genes with a Q -value < 0.05 are indicated by red dots. The number of differentially expressed genes (DEGs) between WT and Slc26a7 −/− was 6371, and that between WT and Slc26a4 −/− was 37. ( c ) Pattern of expression of genes related to thyroid hormone synthesis is depicted using a volcano plot with FC and Q -value. Genes with ≥ 2-fold change and Q -value < 0.05 are indicated by pink dots. ( d ) Expression levels of the genes known to encode iodide transporters in male WT mice (n = 4) using the transcripts per million (TPM) value obtained by StringTie. One-way analysis of variance (ANOVA) showed significant differences among the seven groups ( F (6, 21) = 20.0, p < 0.0001). **** p < 0.0001 determined by Tukey’s test. ( e ) Schema of the relationship between Slc26a7 and Slc26a4 in the thyroid follicular cell, as deduced from the results of this study. WT wild type, CPM counts per million, FC fold change, T3 triiodothyronine, T4 thyroxine, I − iodide.

Article Snippet: Thyroid hormone replacement with albuminized drinking water containing 0.39 mg/L l -thyroxine (L-T4) (L-T4 sodium salt pentahydrate; Med Chem Express, NJ) was started after weaning on day 21.

Techniques: Expressing

Journal: Heliyon

Article Title: The P300-ARRDC3 axis participates in maternal subclinical hypothyroidism and is involved in abnormal hepatic insulin sensitivity in adult offspring

doi: 10.1016/j.heliyon.2024.e39259

Figure Lengend Snippet: Levothyroxine treatment decreases ARRDC3 expression and improves insulin sensitivity in SCH offspring. (A) Blood glucose levels during GTT and (B) ITT were detected in CON + VEH, SCH + VEH, PTU + LT4 mice. The area under the curve (AUC) is shown (n = 10 per group). (C) Serum insulin levels in CON + VEH, SCH + VEH, PTU + LT4 mice under fasting conditions (n = 5 per group). (D) The expression of H3K27Ac and P300 in the liver of three groups was detected by the Western bolt (n = 4 per group). (E)Protein levels of ARRDC3, phosphorylated and total Akt, FoxO1, GSK3β in liver of three groups after insulin injection (2 IU/mouse, 5min) (n = 4 per group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Data are mean ± SEM.

Article Snippet: LT4 (0.1 μg/ml, S2599, Selleck Chemicals, USA) or vehicle (VEH) was added to their drinking water on the basis of previously reported dosing until delivery [ ].

Techniques: Expressing, Western Blot, Injection