Journal: OncoTargets and therapy

Article Title: Carcinogenicity of chromium and chemoprevention: a brief update

doi: 10.2147/OTT.S139262

Figure Lengend Snippet: Summary of carcinogenicity for Cr(III) and Cr(VI)

Article Snippet: In vitro studies , • Few chemoprevention studies conducted • Antioxidants with chemopreventive effects against Cr(III) cyto/genotoxicity: ○ Tempol in human lymphocytes: inhibits cyto/genotoxicity ○ Zinc in PBL: inhibits apoptosis, reduces ROS production, reverses changes in mitochondrial membrane potential, ATP levels, activation of caspase 3 , • Antioxidants with chemopreventive effects against Cr(VI) cyto/genotoxicity: ○ Apple juice in A549: scavenges hydroxyl radicals, reduces lipid peroxidation, DNA damage, cell apoptosis, NFκB activation ○ Quercetin in BEAS2B: decreases ROS generation, inhibits cell transformation, increases miR21 expression, and inhibits of PDCD4 ○ Luteolin in BEAS2B: scavenges ROS ○ EDE in AMJ2-C11: protects against cytotoxicity and oxidative stress ○ Tempol in human lymphocytes: inhibits cyto- and genotoxicity ○ NAC in L02 hepatocytes, H460, HaCaT: inhibits suppression of apoptosis, p53 activation, HSP72 and HIF1α upregulation, prevents ROS-mediated cell death and cytokine expression, decreases rate of cell apoptosis, alleviates mitochondrial and DNA damage – ○ Vitamin C: inhibits increase in DPCs, complete protection against cytotoxicity ○ Lawsonia inermis extract in MDA-MB-435S: scavenges free radicals, inhibits lipid peroxidation ○ Phyllanthus amarus aqueous extract in MDA-MB-435S: inhibits Cr(VI)-induced oxidative toxicity ○ EGCG in BEAS2B: scavenges ROS, protects against DNA damage, inhibits activation of NFκB 7 ○ Melatonin in rat hepatocytes: protects against DSBs, cytotoxicity, lipid peroxidation ○ MCE in BEAS2B, PBL: increases cell viability, decreases DNA damage and ROS level .

Techniques: In Vitro, In Vivo, Activity Assay

Journal: OncoTargets and therapy

Article Title: Carcinogenicity of chromium and chemoprevention: a brief update

doi: 10.2147/OTT.S139262

Figure Lengend Snippet: Summary of chemoprevention for Cr(III) and Cr(VI) carcinogenicity

Article Snippet: In vitro studies , • Few chemoprevention studies conducted • Antioxidants with chemopreventive effects against Cr(III) cyto/genotoxicity: ○ Tempol in human lymphocytes: inhibits cyto/genotoxicity ○ Zinc in PBL: inhibits apoptosis, reduces ROS production, reverses changes in mitochondrial membrane potential, ATP levels, activation of caspase 3 , • Antioxidants with chemopreventive effects against Cr(VI) cyto/genotoxicity: ○ Apple juice in A549: scavenges hydroxyl radicals, reduces lipid peroxidation, DNA damage, cell apoptosis, NFκB activation ○ Quercetin in BEAS2B: decreases ROS generation, inhibits cell transformation, increases miR21 expression, and inhibits of PDCD4 ○ Luteolin in BEAS2B: scavenges ROS ○ EDE in AMJ2-C11: protects against cytotoxicity and oxidative stress ○ Tempol in human lymphocytes: inhibits cyto- and genotoxicity ○ NAC in L02 hepatocytes, H460, HaCaT: inhibits suppression of apoptosis, p53 activation, HSP72 and HIF1α upregulation, prevents ROS-mediated cell death and cytokine expression, decreases rate of cell apoptosis, alleviates mitochondrial and DNA damage – ○ Vitamin C: inhibits increase in DPCs, complete protection against cytotoxicity ○ Lawsonia inermis extract in MDA-MB-435S: scavenges free radicals, inhibits lipid peroxidation ○ Phyllanthus amarus aqueous extract in MDA-MB-435S: inhibits Cr(VI)-induced oxidative toxicity ○ EGCG in BEAS2B: scavenges ROS, protects against DNA damage, inhibits activation of NFκB 7 ○ Melatonin in rat hepatocytes: protects against DSBs, cytotoxicity, lipid peroxidation ○ MCE in BEAS2B, PBL: increases cell viability, decreases DNA damage and ROS level .

Techniques: In Vivo, In Vitro

Journal: Cell host & microbe

Article Title: Exclusive enteral nutrition initiates individual protective microbiome changes to induce remission in pediatric Crohn’s disease

doi: 10.1016/j.chom.2024.10.001

Figure Lengend Snippet:

Article Snippet: L-Ascorbic acid sodium salt (BONCAT dye solution) , ThermoFisher Scientific , Cat#352681000.

Techniques: Recombinant, Preserving, Starch, Guar Gum, Saline, Reverse Transcription, Illumina Sequencing, Software

Journal: PLoS ONE

Article Title: Chemically Defined and Xeno-Free Cryopreservation of Human Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0152161

Figure Lengend Snippet: The post-thaw viability (A) and plating efficiency (B) of hASCs cryopreserved using undefined and defined cryopreservation media. The conventional undefined cryopreservation medium consisted of 10% DMSO+10% DMEM/F12+80% FBS while defined 1 freezing medium was composed of 3.5% DMSO+3.5% EG in DMEM/F-12 containing 0.25 M trehalose, 2% PVA, 5% ficoll, and 0.1 mM EGTA. Defined 2 freezing medium was similar to defined 1 medium but additionally contained two antioxidants (i.e., 3 mM reduced glutathione+5 mM ascorbic acid 2-phosphate). Data shown are mean±SEM. Columns with different letters in each thawing category are significantly different (p < 0.05).

Article Snippet: The osteogenic differentiation medium was DMEM/F-12 containing 10% FBS, 100 nM dexamethasone, 10 mM ß-glycerophosphate (Chem-Impex International), and 0.05 mM L-ascorbic acid-2-phosphate.

Techniques:

Journal: PLoS ONE

Article Title: Chemically Defined and Xeno-Free Cryopreservation of Human Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0152161

Figure Lengend Snippet: The post-thaw viability (A) and plating efficiency (B) of hASCs after further optimization of the defined cryopreservation medium. The undefined cryopreservation medium consisted of 10% DMSO+10% DMEM/F12+80% FBS while defined 2 medium was made up of 3.5% DMSO+3.5% EG+3 mM reduced glutathione+5 mM ascorbic acid 2-phosphate in DMEM/F-12 containing 0.25 M trehalose, 2% PVA, 5% ficoll, and 0.1 mM EGTA. Defined 3 cryopreservation medium had a slightly increased concentration (5%) of DMSO and EG; otherwise it was similar to defined 2 medium). Data shown are mean±SEM. Columns with different letters in each thawing category are significantly different (p < 0.05).

Article Snippet: The osteogenic differentiation medium was DMEM/F-12 containing 10% FBS, 100 nM dexamethasone, 10 mM ß-glycerophosphate (Chem-Impex International), and 0.05 mM L-ascorbic acid-2-phosphate.

Techniques: Concentration Assay

Journal: PLoS ONE

Article Title: Chemically Defined and Xeno-Free Cryopreservation of Human Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0152161

Figure Lengend Snippet: Representative images of ASCs in culture prior to differentiation (A) and after both cryopreservation and differentiation into adipogenic (B), osteogenic (C), and chondrogenic (D) lineages as shown by Oil Red-O, Alizarin Red, and Alcian Blue staining, respectively. In this set of experiments, ASCs were cryopreserved using defined 3 medium (5% DMSO+5% EG+3 mM reduced glutathione+5 mM ascorbic acid 2-phosphate in DMEM/F-12 containing 0.25 M trehalose, 2% PVA, 5% ficoll, and 0.1 mM EGTA). Scale bar = 50 μm.

Article Snippet: The osteogenic differentiation medium was DMEM/F-12 containing 10% FBS, 100 nM dexamethasone, 10 mM ß-glycerophosphate (Chem-Impex International), and 0.05 mM L-ascorbic acid-2-phosphate.

Techniques: Staining

Journal: PLoS ONE

Article Title: Chemically Defined and Xeno-Free Cryopreservation of Human Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0152161

Figure Lengend Snippet: Normal human female karyotype of ASCs cryopreserved under defined conditions at passage 6 using defined 3 cryopreservation medium (5% DMSO+5% EG+3 mM reduced glutathione+5 mM ascorbic acid 2-phosphate in DMEM/F-12 containing 0.25 M trehalose, 2% PVA, 5% ficoll, and 0.1 mM EGTA).

Article Snippet: The osteogenic differentiation medium was DMEM/F-12 containing 10% FBS, 100 nM dexamethasone, 10 mM ß-glycerophosphate (Chem-Impex International), and 0.05 mM L-ascorbic acid-2-phosphate.

Techniques: