Journal: mSphere

Article Title: L-arginine supplementation abrogates hypoxia-induced virulence of Staphylococcus aureus in a murine diabetic pressure wound model

doi: 10.1128/msphere.00774-23

Figure Lengend Snippet: Dietary L-Arg supplementation maintains body weight and serum glucose and increases ROS and nitrate production in diabetic TALLYHO/JngJ mice. ( A ) Body weight and ( B ) serum glucose level in diabetic TALLYHO/JngJ mice before and after gavage with PBS ( n = 12) or L-Arg for 6 weeks ( n = 35). ( C ) Serum nitrite/nitrate (NOx) level and ( D ) ROS response of bone marrow-derived neutrophils from diabetic TALLYHO/JngJ mice after gavage with PBS or L-Arg for 6 weeks. ( E ) Representative images of in vivo ROS/RNS response to pressure wound visualized by L-012 luminol. The alphabet indicates statistical significance determined by the Mann-Whitney test ( P < 0.0001). The asterisk indicates statistical significance determined by the Student’s t -test. * P < 0.01.

Article Snippet: To measure in vivo ROS response from a pressure wound, a ROS-sensing probe, L-012 luminol (10 mg/mL, Labchem Wako) stock solution, was prepared in normal saline and intraperitoneally injected with 10 mL/kg of stock solution to achieve a final dosage of 100 mg/kg ( ).

Techniques: Derivative Assay, In Vivo, MANN-WHITNEY