Journal: Brazilian Journal of Medical and Biological Research
Article Title: Cardiac function and intracellular Ca 2+ handling proteins are not impaired by high-saturated-fat diet-induced obesity
Figure Lengend Snippet: Western blot analysis of intracellular Ca 2+ -cycling proteins in myocardium from control (C, white bars) and obese (Ob, black bars) rats (n=6 in each group). A , Quantification of sarcoplasmic reticulum Ca 2+ -ATPase (SERCA2a), phospholamban (PLB), PLB serine-16 phosphorylation (pPLB Ser 16 ), PLB threonine-17 phosphorylation (pPLB Thr 17 ), ryanodine receptor (RYR), calsequestrin (CSQ), Na + /Ca 2+ exchanger (NCX), and L-type Ca 2+ channel. B , Representative bands of proteins evaluated by western blot. Data are reported as means±SD. Student's t -test for independent samples was used to compare data.
Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies against SERCA2a (1:2,500; ABR Affinity BioReagents, USA), PLB (1:1,000, Abcam, USA), pPLB Ser 16 (1:5,000; Badrilla, UK), pPLB Thr 17 (1:5,000; Badrilla), RYR (1:5,000; ABR Affinity BioReagents), CSQ (1:300; ABR Affinity BioReagents), NCX (1:1,000; Upstate, USA), and L-type Ca 2+ channel alpha 1C (1:100; Chemicon International, USA).
Techniques: Western Blot