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    Alomone Labs kv1 5
    Myocardial Kvβ2 associates with Kv1 and Kv4 proteins. ( A ) Representative blot images showing immunoreactive bands for Kv1.4, <t>Kv1.5,</t> Kv4.2, Kv4.3 and Kvβ2 in whole heart lysates and Kvβ2 immunoprecipitates. Absence of immunoreactive bands at expected molecular weights for each protein is also shown for mouse IgG immunoprecipitates as a negative control. Representative of 3 independent experiments. ( B ) Representative blot image showing immunoreactivity for Kvβ2 (predicted molecular weight, ~37 kDa) in Kvβ2 immunoprecipitates from heart lysates of wild type and Kvβ2 −/− animals. ( C ) Differential interference contrast (DIC) and proximity ligation assay (PLA)-associated fluorescence images of isolated adult ventricular myocytes from wild type and Kvβ2 −/− animals after PLA targeting of Kvβ2 complexes using mouse and rabbit-derived anti-Kvβ2 primary antibodies. PLA images are shown as flattened 2D maximum intensity z-projections from z-series captured for each cell. ( D ) PLA images of isolated ventricular myocytes treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1 anti-Kv4.2, anti-Kv4.3. ( E ) Summary of fluorescent PLA-associated punctae, normalized to cell footprint area, for cells treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1, anti-Kv4.2 and anti-Kv4.3 primary antibodies (n = 6–10). *P
    Kv1 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kv1 5/product/Alomone Labs
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    kv1 5 - by Bioz Stars, 2022-10
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    88
    Upstate Biotechnology Inc kv1 5
    Myocardial Kvβ2 associates with Kv1 and Kv4 proteins. ( A ) Representative blot images showing immunoreactive bands for Kv1.4, <t>Kv1.5,</t> Kv4.2, Kv4.3 and Kvβ2 in whole heart lysates and Kvβ2 immunoprecipitates. Absence of immunoreactive bands at expected molecular weights for each protein is also shown for mouse IgG immunoprecipitates as a negative control. Representative of 3 independent experiments. ( B ) Representative blot image showing immunoreactivity for Kvβ2 (predicted molecular weight, ~37 kDa) in Kvβ2 immunoprecipitates from heart lysates of wild type and Kvβ2 −/− animals. ( C ) Differential interference contrast (DIC) and proximity ligation assay (PLA)-associated fluorescence images of isolated adult ventricular myocytes from wild type and Kvβ2 −/− animals after PLA targeting of Kvβ2 complexes using mouse and rabbit-derived anti-Kvβ2 primary antibodies. PLA images are shown as flattened 2D maximum intensity z-projections from z-series captured for each cell. ( D ) PLA images of isolated ventricular myocytes treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1 anti-Kv4.2, anti-Kv4.3. ( E ) Summary of fluorescent PLA-associated punctae, normalized to cell footprint area, for cells treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1, anti-Kv4.2 and anti-Kv4.3 primary antibodies (n = 6–10). *P
    Kv1 5, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kv1 5/product/Upstate Biotechnology Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kv1 5 - by Bioz Stars, 2022-10
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    80
    Upstate Biotechnology Inc anti kv1 5 antibodies
    Expression of Kv1.2 and <t>Kv1.5</t> channel α subunits in mouse sciatic nerve during postnatal development. A , Left , Membrane fractions of sciatic nerves from P1, P4, P8, and P40 mice were subjected to SDS-PAGE and immunoblot analysis with anti-Kv1.5 antibodies. To estimate and compare total protein inputs in each lane, blots were stained with Ponceau S before immunoblot analysis (data not shown). Right , RT-PCR, followed by Southern blot analysis of Kv1.5 transcripts in sciatic nerves from P1 and P40 mice. B , Left , Immunoblot analysis of Kv1.2 on postnatal sciatic nerve as in A . Right , RT-PCR and Southern blot analysis of Kv1.2 as in A . Primer pairs to the specific 3′ coding regions of either Kv1.5 ( A ) or Kv1.2 ( B ) amplified PCR fragments of 273 and 248 bp, respectively. The bottom band represents the S16 ribosomal protein PCR fragment (102 bp), which was used to estimate the starting input RNA. C , Quantitation of the developmental downregulation of Kv1.2 and Kv1.5 proteins from P1 to P40 sciatic nerve as illustrated in A and B . Data of densitometric scanning were normalized to values of P40 and represent mean ± SEM of three independent experiments. * p
    Anti Kv1 5 Antibodies, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 5 antibodies/product/Upstate Biotechnology Inc
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv1 5 antibodies - by Bioz Stars, 2022-10
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    Image Search Results


    Myocardial Kvβ2 associates with Kv1 and Kv4 proteins. ( A ) Representative blot images showing immunoreactive bands for Kv1.4, Kv1.5, Kv4.2, Kv4.3 and Kvβ2 in whole heart lysates and Kvβ2 immunoprecipitates. Absence of immunoreactive bands at expected molecular weights for each protein is also shown for mouse IgG immunoprecipitates as a negative control. Representative of 3 independent experiments. ( B ) Representative blot image showing immunoreactivity for Kvβ2 (predicted molecular weight, ~37 kDa) in Kvβ2 immunoprecipitates from heart lysates of wild type and Kvβ2 −/− animals. ( C ) Differential interference contrast (DIC) and proximity ligation assay (PLA)-associated fluorescence images of isolated adult ventricular myocytes from wild type and Kvβ2 −/− animals after PLA targeting of Kvβ2 complexes using mouse and rabbit-derived anti-Kvβ2 primary antibodies. PLA images are shown as flattened 2D maximum intensity z-projections from z-series captured for each cell. ( D ) PLA images of isolated ventricular myocytes treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1 anti-Kv4.2, anti-Kv4.3. ( E ) Summary of fluorescent PLA-associated punctae, normalized to cell footprint area, for cells treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1, anti-Kv4.2 and anti-Kv4.3 primary antibodies (n = 6–10). *P

    Journal: Journal of molecular and cellular cardiology

    Article Title: Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits

    doi: 10.1016/j.yjmcc.2019.09.013

    Figure Lengend Snippet: Myocardial Kvβ2 associates with Kv1 and Kv4 proteins. ( A ) Representative blot images showing immunoreactive bands for Kv1.4, Kv1.5, Kv4.2, Kv4.3 and Kvβ2 in whole heart lysates and Kvβ2 immunoprecipitates. Absence of immunoreactive bands at expected molecular weights for each protein is also shown for mouse IgG immunoprecipitates as a negative control. Representative of 3 independent experiments. ( B ) Representative blot image showing immunoreactivity for Kvβ2 (predicted molecular weight, ~37 kDa) in Kvβ2 immunoprecipitates from heart lysates of wild type and Kvβ2 −/− animals. ( C ) Differential interference contrast (DIC) and proximity ligation assay (PLA)-associated fluorescence images of isolated adult ventricular myocytes from wild type and Kvβ2 −/− animals after PLA targeting of Kvβ2 complexes using mouse and rabbit-derived anti-Kvβ2 primary antibodies. PLA images are shown as flattened 2D maximum intensity z-projections from z-series captured for each cell. ( D ) PLA images of isolated ventricular myocytes treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1 anti-Kv4.2, anti-Kv4.3. ( E ) Summary of fluorescent PLA-associated punctae, normalized to cell footprint area, for cells treated with anti-Kvβ2 only, or anti-Kvβ2 with anti-Kv1.4, anti-Kv1.5, anti-Kv2.1, anti-Kv4.2 and anti-Kv4.3 primary antibodies (n = 6–10). *P

    Article Snippet: [ ] [ ] [52] Tipparaju SM, Li XP, Kilfoil PJ, Xue B, Uversky VN, Bhatnagar A, Barski OA, Interactions between the C-terminus of Kv1.5 and Kvbeta regulate pyridine nucleotidedependent changes in channel gating , Pflugers Arch 463 ( 6 ) (2012) 799–818.

    Techniques: Negative Control, Molecular Weight, Proximity Ligation Assay, Fluorescence, Isolation, Derivative Assay

    Kvβ2 promotes Kv1 and Kv4 surface expression in cardiac myocytes. ( A ) Differential interference contrast (DIC) and confocal images showing Kvβ2-associated fluorescence (red) in isolated cardiac myocytes from wild type (wt) and Kvβ2 −/− animals. Nuclei (dapi) are shown in blue. ( B ) Western blots showing immunoreactive bands for Kv pore-forming and auxiliary subunits at respective predicted molecular weights as indicated in heart lysates from wt (n = 3) and Kvβ2 −/− (n = 3) animals. As a representative loading control, immunoreactive bands for GAPDH are shown for each lane of Kv1.5 blot. ( C ) Summarized densitometric data for Kv1.4, Kv1.5, Kv2.1, Kv4.2, Kv4.3, Kvβ1.1, Kvβ1.2, Kvβ2, and KChIP2 proteins in heart lysates of wt animals. Data are normalized to GAPDH (run for each blot) and expressed relative to wt controls. *P

    Journal: Journal of molecular and cellular cardiology

    Article Title: Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits

    doi: 10.1016/j.yjmcc.2019.09.013

    Figure Lengend Snippet: Kvβ2 promotes Kv1 and Kv4 surface expression in cardiac myocytes. ( A ) Differential interference contrast (DIC) and confocal images showing Kvβ2-associated fluorescence (red) in isolated cardiac myocytes from wild type (wt) and Kvβ2 −/− animals. Nuclei (dapi) are shown in blue. ( B ) Western blots showing immunoreactive bands for Kv pore-forming and auxiliary subunits at respective predicted molecular weights as indicated in heart lysates from wt (n = 3) and Kvβ2 −/− (n = 3) animals. As a representative loading control, immunoreactive bands for GAPDH are shown for each lane of Kv1.5 blot. ( C ) Summarized densitometric data for Kv1.4, Kv1.5, Kv2.1, Kv4.2, Kv4.3, Kvβ1.1, Kvβ1.2, Kvβ2, and KChIP2 proteins in heart lysates of wt animals. Data are normalized to GAPDH (run for each blot) and expressed relative to wt controls. *P

    Article Snippet: [ ] [ ] [52] Tipparaju SM, Li XP, Kilfoil PJ, Xue B, Uversky VN, Bhatnagar A, Barski OA, Interactions between the C-terminus of Kv1.5 and Kvbeta regulate pyridine nucleotidedependent changes in channel gating , Pflugers Arch 463 ( 6 ) (2012) 799–818.

    Techniques: Expressing, Fluorescence, Isolation, Western Blot

    Expression of Kv1.2 and Kv1.5 channel α subunits in mouse sciatic nerve during postnatal development. A , Left , Membrane fractions of sciatic nerves from P1, P4, P8, and P40 mice were subjected to SDS-PAGE and immunoblot analysis with anti-Kv1.5 antibodies. To estimate and compare total protein inputs in each lane, blots were stained with Ponceau S before immunoblot analysis (data not shown). Right , RT-PCR, followed by Southern blot analysis of Kv1.5 transcripts in sciatic nerves from P1 and P40 mice. B , Left , Immunoblot analysis of Kv1.2 on postnatal sciatic nerve as in A . Right , RT-PCR and Southern blot analysis of Kv1.2 as in A . Primer pairs to the specific 3′ coding regions of either Kv1.5 ( A ) or Kv1.2 ( B ) amplified PCR fragments of 273 and 248 bp, respectively. The bottom band represents the S16 ribosomal protein PCR fragment (102 bp), which was used to estimate the starting input RNA. C , Quantitation of the developmental downregulation of Kv1.2 and Kv1.5 proteins from P1 to P40 sciatic nerve as illustrated in A and B . Data of densitometric scanning were normalized to values of P40 and represent mean ± SEM of three independent experiments. * p

    Journal: The Journal of Neuroscience

    Article Title: Heteromultimeric Delayed-Rectifier K+ Channels in Schwann Cells: Developmental Expression and Role in Cell Proliferation

    doi: 10.1523/JNEUROSCI.18-24-10398.1998

    Figure Lengend Snippet: Expression of Kv1.2 and Kv1.5 channel α subunits in mouse sciatic nerve during postnatal development. A , Left , Membrane fractions of sciatic nerves from P1, P4, P8, and P40 mice were subjected to SDS-PAGE and immunoblot analysis with anti-Kv1.5 antibodies. To estimate and compare total protein inputs in each lane, blots were stained with Ponceau S before immunoblot analysis (data not shown). Right , RT-PCR, followed by Southern blot analysis of Kv1.5 transcripts in sciatic nerves from P1 and P40 mice. B , Left , Immunoblot analysis of Kv1.2 on postnatal sciatic nerve as in A . Right , RT-PCR and Southern blot analysis of Kv1.2 as in A . Primer pairs to the specific 3′ coding regions of either Kv1.5 ( A ) or Kv1.2 ( B ) amplified PCR fragments of 273 and 248 bp, respectively. The bottom band represents the S16 ribosomal protein PCR fragment (102 bp), which was used to estimate the starting input RNA. C , Quantitation of the developmental downregulation of Kv1.2 and Kv1.5 proteins from P1 to P40 sciatic nerve as illustrated in A and B . Data of densitometric scanning were normalized to values of P40 and represent mean ± SEM of three independent experiments. * p

    Article Snippet: Interestingly, when Kv1.5 α subunits were depleted from the P4 sciatic nerve extracts with anti-Kv1.5 antibodies, no Kv1.2 subunit could subsequently be immunoprecipitated by anti-Kv1.2 antibodies, suggesting that most of the Kv1.2 α subunits are involved in heteromultimeric association with Kv1.5 subunits.

    Techniques: Expressing, Mouse Assay, SDS Page, Staining, Reverse Transcription Polymerase Chain Reaction, Southern Blot, Amplification, Polymerase Chain Reaction, Quantitation Assay

    The heteromeric association of Kv1.2 and Kv1.5 channel α subunits in sciatic nerve. Reciprocal coimmunoprecipitation of Kv1.5 and Kv1.2 in P4 sciatic nerve is shown. A , Homogenates of acutely isolated sciatic nerves from P4 mice were subjected to immunoprecipitation with anti-Kv1.2, anti-Kv1.5, anti-Kv3.1, and preimmune antibodies. The Kv1.2 and Kv1.5 subunits were depleted from sciatic nerve extracts with their respective antibodies, and unbound proteins were subjected to a second round of immunoprecipitation with anti-Kv1.2 ( Kv1.5 depl. Kv1.2 ) and anti-Kv1.5 ( Kv1.2 depl. Kv1.5 ), respectively. Blots were probed with anti-Kv1.5. B , Reciprocal coimmunoprecipitation of Kv1.2 with Kv1.5 in P4 sciatic nerve. The blot shown in A was stripped and reprobed with anti-Kv1.2.

    Journal: The Journal of Neuroscience

    Article Title: Heteromultimeric Delayed-Rectifier K+ Channels in Schwann Cells: Developmental Expression and Role in Cell Proliferation

    doi: 10.1523/JNEUROSCI.18-24-10398.1998

    Figure Lengend Snippet: The heteromeric association of Kv1.2 and Kv1.5 channel α subunits in sciatic nerve. Reciprocal coimmunoprecipitation of Kv1.5 and Kv1.2 in P4 sciatic nerve is shown. A , Homogenates of acutely isolated sciatic nerves from P4 mice were subjected to immunoprecipitation with anti-Kv1.2, anti-Kv1.5, anti-Kv3.1, and preimmune antibodies. The Kv1.2 and Kv1.5 subunits were depleted from sciatic nerve extracts with their respective antibodies, and unbound proteins were subjected to a second round of immunoprecipitation with anti-Kv1.2 ( Kv1.5 depl. Kv1.2 ) and anti-Kv1.5 ( Kv1.2 depl. Kv1.5 ), respectively. Blots were probed with anti-Kv1.5. B , Reciprocal coimmunoprecipitation of Kv1.2 with Kv1.5 in P4 sciatic nerve. The blot shown in A was stripped and reprobed with anti-Kv1.2.

    Article Snippet: Interestingly, when Kv1.5 α subunits were depleted from the P4 sciatic nerve extracts with anti-Kv1.5 antibodies, no Kv1.2 subunit could subsequently be immunoprecipitated by anti-Kv1.2 antibodies, suggesting that most of the Kv1.2 α subunits are involved in heteromultimeric association with Kv1.5 subunits.

    Techniques: Isolation, Mouse Assay, Immunoprecipitation