ktrb Search Results


rabbit antibodies against ktrb  (Qiagen)
90
Qiagen rabbit antibodies against ktrb
a <t>)</t> <t>SDS-PAGE</t> analysis of fractions collected from a pulldown experiment with Streptactin beads. Pulldown was performed with a lysate of E. coli cells (Left) co-expressing N-terminally Strep-tagged KtrA ( Strep KtrA) and C-terminally His-tagged KtrC (KtrC His ) or (Right) expressing KtrC His alone, showing that KtrC His is present in the elution fractions only in the co-expression experiment. Lysare (input), washes (W1,W2), elution with desthiobiotin. b ) SDS-PAGE analysis of eluted fractions from pulldown experiments performed with samples collected at various time points from a mixture of Strep KtrA and KtrC (independently purified) prepared and incubated in the presence of ATP (top) and ADP (bottom) c ) Pyranine fluorescence flux curves for Ktr channels assembled by addition of KtrA, KtrC or heteromeric Strep KtrA/C to <t>KtrB-reconstituted</t> liposomes in the presence of ATP and Mg 2+ with and without 20 µM c-di-AMP. d) Example of pyranine fluorescence flux curves resulting from titration of assembled KtrA/CB with increasing concentrations of c-di-AMP. e) Plot of time constants extracted from curves in d) as a function of c-di-AMP concentration. Data were fitted with Hill equation (red curve) with the following parameters, Ki= 2.8 ±0.6 μM and Hill coefficient n= 1.1 ±0.4. For comparison, we show the fitted curve (black line) to the c-di-AMP titration of KtrCB (from Figure 2d). Mean ±SD from n=3.
Rabbit Antibodies Against Ktrb, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ktrb  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies ktrb
a <t>)</t> <t>SDS-PAGE</t> analysis of fractions collected from a pulldown experiment with Streptactin beads. Pulldown was performed with a lysate of E. coli cells (Left) co-expressing N-terminally Strep-tagged KtrA ( Strep KtrA) and C-terminally His-tagged KtrC (KtrC His ) or (Right) expressing KtrC His alone, showing that KtrC His is present in the elution fractions only in the co-expression experiment. Lysare (input), washes (W1,W2), elution with desthiobiotin. b ) SDS-PAGE analysis of eluted fractions from pulldown experiments performed with samples collected at various time points from a mixture of Strep KtrA and KtrC (independently purified) prepared and incubated in the presence of ATP (top) and ADP (bottom) c ) Pyranine fluorescence flux curves for Ktr channels assembled by addition of KtrA, KtrC or heteromeric Strep KtrA/C to <t>KtrB-reconstituted</t> liposomes in the presence of ATP and Mg 2+ with and without 20 µM c-di-AMP. d) Example of pyranine fluorescence flux curves resulting from titration of assembled KtrA/CB with increasing concentrations of c-di-AMP. e) Plot of time constants extracted from curves in d) as a function of c-di-AMP concentration. Data were fitted with Hill equation (red curve) with the following parameters, Ki= 2.8 ±0.6 μM and Hill coefficient n= 1.1 ±0.4. For comparison, we show the fitted curve (black line) to the c-di-AMP titration of KtrCB (from Figure 2d). Mean ±SD from n=3.
Ktrb, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ktrb/product/Federation of European Neuroscience Societies
Average 90 stars, based on 1 article reviews
ktrb - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


a ) SDS-PAGE analysis of fractions collected from a pulldown experiment with Streptactin beads. Pulldown was performed with a lysate of E. coli cells (Left) co-expressing N-terminally Strep-tagged KtrA ( Strep KtrA) and C-terminally His-tagged KtrC (KtrC His ) or (Right) expressing KtrC His alone, showing that KtrC His is present in the elution fractions only in the co-expression experiment. Lysare (input), washes (W1,W2), elution with desthiobiotin. b ) SDS-PAGE analysis of eluted fractions from pulldown experiments performed with samples collected at various time points from a mixture of Strep KtrA and KtrC (independently purified) prepared and incubated in the presence of ATP (top) and ADP (bottom) c ) Pyranine fluorescence flux curves for Ktr channels assembled by addition of KtrA, KtrC or heteromeric Strep KtrA/C to KtrB-reconstituted liposomes in the presence of ATP and Mg 2+ with and without 20 µM c-di-AMP. d) Example of pyranine fluorescence flux curves resulting from titration of assembled KtrA/CB with increasing concentrations of c-di-AMP. e) Plot of time constants extracted from curves in d) as a function of c-di-AMP concentration. Data were fitted with Hill equation (red curve) with the following parameters, Ki= 2.8 ±0.6 μM and Hill coefficient n= 1.1 ±0.4. For comparison, we show the fitted curve (black line) to the c-di-AMP titration of KtrCB (from Figure 2d). Mean ±SD from n=3.

Journal: bioRxiv

Article Title: c-di-AMP determines the hierarchical organization of bacterial RCK proteins

doi: 10.1101/2023.04.24.538052

Figure Lengend Snippet: a ) SDS-PAGE analysis of fractions collected from a pulldown experiment with Streptactin beads. Pulldown was performed with a lysate of E. coli cells (Left) co-expressing N-terminally Strep-tagged KtrA ( Strep KtrA) and C-terminally His-tagged KtrC (KtrC His ) or (Right) expressing KtrC His alone, showing that KtrC His is present in the elution fractions only in the co-expression experiment. Lysare (input), washes (W1,W2), elution with desthiobiotin. b ) SDS-PAGE analysis of eluted fractions from pulldown experiments performed with samples collected at various time points from a mixture of Strep KtrA and KtrC (independently purified) prepared and incubated in the presence of ATP (top) and ADP (bottom) c ) Pyranine fluorescence flux curves for Ktr channels assembled by addition of KtrA, KtrC or heteromeric Strep KtrA/C to KtrB-reconstituted liposomes in the presence of ATP and Mg 2+ with and without 20 µM c-di-AMP. d) Example of pyranine fluorescence flux curves resulting from titration of assembled KtrA/CB with increasing concentrations of c-di-AMP. e) Plot of time constants extracted from curves in d) as a function of c-di-AMP concentration. Data were fitted with Hill equation (red curve) with the following parameters, Ki= 2.8 ±0.6 μM and Hill coefficient n= 1.1 ±0.4. For comparison, we show the fitted curve (black line) to the c-di-AMP titration of KtrCB (from Figure 2d). Mean ±SD from n=3.

Article Snippet: Pulled-down fractions were analysed by SDS-PAGE or Western blot using rabbit antibodies against KtrB or mouse against His-tag (Qiagen).

Techniques: SDS Page, Expressing, Purification, Incubation, Fluorescence, Titration, Concentration Assay