Journal: bioRxiv

Article Title: Comparative analysis of varicella-zoster virus and herpes simplex virus 1 interaction with epidermal terminal differentiation in primary human keratinocytes models of differentiation

doi: 10.64898/2026.04.08.717198

Figure Lengend Snippet: A. Schematic of plan of infection with HSV-1 at MOI 0.005 of undifferentiated (Undiff.), early differentiated (Early diff.) and late differentiated (Late diff.) NHEKs. “Undiff.”, “Early diff.” and “Late diff.” labelling refers to the state of NHEKs differentiation at the time of infection (day 0). The “Undiff.” condition includes the NHEKs undergoing spontaneous asynchronous differentiation after infection (Differentiating_async.) B. Analysis by qPCR of cell-associated HSV-1 genome copy number normalised to number of cells, upon infection with HSV-1 of Undiff., Early diff. and Late diff. NHEKs, and evaluated at 24h, 48h and 72h p.i. The data are generated from quantification of the HSV-1 UL27 gene (gB) normalised to RPP30 gene (host internal control) and are reported as mean of n=3 independent experiments ± SEM. C. Analysis by qPCR of cell-associated HSV-1 genome copy number normalised to number of cells, upon infection with HSV-1 of Late diff. NHEKs, and reported at 72h and 120h p.i. The data are generated from quantification of the HSV-1 UL27 gene (gB) normalised to RPP30 gene (host internal control) and are reported as the mean of three technical replicates. D. ICP0 (pink) immunofluorescence staining of Undiff., Early diff. and Late diff. NHEKs infected with HSV-1 and analysed at 4h, 24h and 72h p.i. The reported images include GFP (green) signal deriving from the GFP protein tagged to the late HSV-1 tegument protein UL46 and DAPI (grey) staining of nuclei. E. Analysis by qRT-PCR analysis of RL2 gene (encoding the ICP0 protein) and UL48 gene (encoding the VP16 protein) expression in HSV-1 infection of Undiff., Early diff. and Late diff. NHEKs, and reported at 24h, 48h and 72h p.i. The data are reported as mean of 2 -dCT of n=3 independent experiments ± SEM, where the normalisation was performed against GAPDH . F. Western Blotting analysis of ICP0 and VP16 expression at 24h, 48h and 72h p.i. following infection of Undiff., Early diff. and Late diff. NHEKs with HSV-1. GAPDH was used as loading control. Data are representative of n=4 independent experiments (Undiff. condition), n=3 independent experiments (Early diff. condition) and n=2 independent experiments (Late diff. condition). G. Schematic of the model of HSV-1 infection implemented in undifferentiated NHEKs, which either remained undifferentiated throughout the infection and until the last time point (72h) (Undifferentiated) or started undergoing spontaneous differentiation during the last time points of infection (Differentiating_async). H. Analysis by qRT-PCR of KRT5 and KRT10 mRNAs in uninfected NHEKs at 72h confirming the undifferentiated and differentiating status of the keratinocytes. The data are reported as fold change (FC) (2 -ddCT ) to the undifferentiated condition and are the mean of three technical replicates, where the normalisation was performed against GAPDH . I. Keratinocytes where infected when undifferentiated and then either maintained their undifferentiated status throughout the infection (Undifferentiated) or were allowed to spontaneously differentiate by 72h p.i. (Differentiating). Cell-associated HSV-1 genome copy number was analysed at 72h p.i. in both conditions. The data are generated from quantification of the HSV-1 UL27 gene (gB) normalised to RPP30 gene (host internal control), are reported as the mean of three technical replicates and are representative of n=3 independent experiments. J. Anlysis by qRT-PCR of RL2 and UL48 mRNAs in HSV-1 infected Undifferentiated and Differentiating NHEKs at 72h p.i. The data are reported as mean of 2 -dCT of three technical replicates, where the normalisation was performed against GAPDH . Data are representative of n=3 independent experiments. Statistical significance was evaluated in B. and E. by 2way ANOVA with Tukey’s multiple comparisons test and indicated as *P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001. In D. , dotted lines mark nuclei at 4h p.i. Insets contain zoomed-in areas of the ICP0 signal inside the nuclei at 4h p.i. Scale bar, 10 μm. Undiff., undifferentiated; Early diff., early differentiated; Late diff., late differentiated; p.i., post infection; NHEKs, normal human epidermal keratinocytes.

Article Snippet: The primers and probes used for host genes were purchased from Thermofisher Scientific, cat n 4448489 (VIC dye): GAPDH (ASSAY ID Hs02786624_g1) and cat n 4331182 (FAM dye): KRT10 (assay ID Hs00166289_m1), KRT1 (assay ID Hs00196158_m1), KRT5 (assay ID Hs00361185_m1), LOR (assay ID Hs01894962_s1), FLG (assay ID Hs00856927_g1), LCE3D (assay ID Hs00754375_s1), DSC1 (assay ID Hs00245189_m1).

Techniques: Infection, Generated, Control, Immunofluorescence, Staining, Quantitative RT-PCR, Expressing, Western Blot

Journal: Nature Communications

Article Title: Tuft cells shape airway remodeling by eliciting OXGR1- and SOX9-dependent stem cell programs

doi: 10.1038/s41467-026-70763-y

Figure Lengend Snippet: a Representative images of DCLK1 and BCAM staining in tracheal sections from the indicated Sox9-tdT reporter strains at day 14. Sox9-tdT + BCAM + cells (white arrow). Scale bars: 50 µm. b Quantification of Sox9-tdT + BCAM + cells in the SAE from the indicated strains at day 14. Group sizes: n = 5, 6, 12, 5 mice, from left to right. Two-tailed unpaired Mann-Whitney U test. c , d Combined in situ hybridization (ISH) for Oxgr1, Krt5 and Krt8 , and immunofluorescence for SOX9 and α-SMA in tracheal sections from WT mice with or without ALT treatment ( Krt5 for BCs, Krt8 for differentiated cells, SOX9 for SMG progenitor cells, and α-SMA for SMG myoepithelial cells). Oxgr1 + SOX9 + cells (green arrows). Scale bars: 20 μm. SMG Submucosal gland. ( e ) Quantification of Oxgr1 + cells in the specified EpC subsets. Group sizes: n = 7, 16, 8, 13, 6, 10, 7, 8 mice, from left to right. Kruskal–Wallis test followed by Dunn’s multiple-comparisons test with Holm–Bonferroni correction. f Quantification of Oxgr1 + cells among the Krt5 + or Krt8 + cells. Group sizes: n = 12, 11, 12, 7 mice, from left to right. Two-tailed unpaired Mann-Whitney U test. g Experimental schema. h , i Representative images ( h ) and quantification ( i ) of BCAM and SOX9 staining in tracheal sections from the indicated strains at day 14. Scale bars: 20 μm. Group sizes: n = 5, 8, 7, 5 mice, from left to right. Kruskal–Wallis test followed by Dunn’s multiple-comparisons test with Holm–Bonferroni correction. The dashed line marks the border between SAE and SM. Box plots ( b , e , f , i ) show median (center line), 25th–75th percentiles (box), and min–max values (whiskers). Each data point represents the average of 3–4 images from one mouse. See “Methods”. Panel g was created in BioRender. Lee, M. ( https://BioRender.com/dg3i7rm ). Source data are provided as a Source Data file.

Article Snippet: Female (12 weeks- to 14 weeks) and male (10 weeks- to 12 weeks) mice of the following genotypes and strains were used: C57BL/6 (Charles River Laboratories, CRL# 027), Oxgr1 -/- (KOMP, stock number 048933-UCD), Pou2f3 -/- (The Jackson Laboratory, stock number 037040), Cysltr1 -/- (kindly provided by Dr. Lora Bankova, BWH, Boston), Cysltr2 -/- (kindly provided by Dr. Joshua Boyce, BWH, Boston), Krt5 CreERT2 (The Jackson Laboratory, stock number 029155), Pou2f3 CreERT2 (The Jackson Laboratory, stock number 037511), Sox9 CreERT2 (The Jackson Laboratory, stock number 035092), Sox9 fl/fl (The Jackson Laboratory, stock number 013106), Oxgr1 fl/fl (Barrett Lab), Ltc4s fl/fl (kindly provided by Dr. Lora Bankova, BWH, Boston), and Ai9 (RCL-tdT) (The Jackson Laboratory, stock number 007909).

Techniques: Staining, Two Tailed Test, MANN-WHITNEY, In Situ Hybridization, Immunofluorescence

Journal: Cell Death & Disease

Article Title: A novel super-enhancer-driven lncRNA LINC00973 governs head and neck squamous cell carcinoma progression through EN2

doi: 10.1038/s41419-025-08380-8

Figure Lengend Snippet: a The relative expression of LINC00973 in normal human oral keratinocytes (HOK) cell and HNSCC cell lines were measured by qRT-PCR. b Subcellular RNA fraction and followed qRT-PCR assays were conducted to determine the localization of LINC00973 within Cal27 and HN6 cells. GAPDH and U6 served as the internal control of cytoplasm and nuclear, respectively. c RNA FISH assays showed LINC00973 mainly localized in cytoplasm in Cal27 and HN6 cells. Scale bar: 10 μm. d The knockdown efficiency of LINC00973 was determined by qRT-PCR in Cal27 and HN6 cells transfected si-LINC00973-1 (si-1) and si-LINC00973-2 (si-2). Cell proliferation, migration, and invasion was evaluated by CCK-8 ( e ), wound-healing ( f ), and Transwell invasion assays ( g ) in LINC00973-sliencing Cal27 and HN6 cells. Scale bar: 50 μm. h The protein abundance of indicated migration and invasion related markers was detected in LINC00973-deficient cells. i Schematic description of experimental procedures for the orthotopic floor-of-mouth xenograft model. j Representative image of the tumor masses with stable LINC00973 knockdown/control HN6 cells and the final volume of samples were recorded. Scale bar: 1 cm. k Representative IHC staining of Ki67 in xenograft tumors (left panel) and their quantification data were shown (right panel). Scale bar: 50 μm. l Representative H&E staining and IHC staining of CK5/6 in lymph nodes (left panel) from orthotopic tumor-bearing mice. The percentage of lymph nodes metastasis were shown and compared (right panel). Fisher’s exact test. Data shown here are mean ± SD from three independent experiments, ** P < 0.01, Student’s t or one-way ANOVA test.

Article Snippet: After blocking with QuickBlock Blocking Buffer (#P0220, Beyotime, China), sections were incubated overnight at 4 °C with primary antibodies against CK5/6 (#MAB-0744, Maixin, China), Ki67 (#MAB-0672, Maixin, China), EN2 (1:100, #sc-293311, Santa Cruz, USA), FOSL1 (1:1000, #5281, Cell signaling, USA) or NOTCH1 (1:500, #20687-1-AP, Proteintech, China).

Techniques: Expressing, Quantitative RT-PCR, Control, Knockdown, Transfection, Migration, CCK-8 Assay, Quantitative Proteomics, Immunohistochemistry, Staining

Journal: Cell Death & Disease

Article Title: A novel super-enhancer-driven lncRNA LINC00973 governs head and neck squamous cell carcinoma progression through EN2

doi: 10.1038/s41419-025-08380-8

Figure Lengend Snippet: Heatmap ( a ) and volcano plot ( b ) displayed the expression profile of DEGs following LINC00973 knockdown or control cells. The mRNA ( c ) and protein ( d ) expression of EN2 were measured in Cal27 and HN6 cells with/without EN2 knockdown. e , f The correlations between EN2 and LINC00973 RNA expression were assessed in the public HNSCC datasets ( GSE41613 and GSE42743 ) and our in-house samples. Spearman’s correlation. The mRNA ( g ) and protein ( h ) abundance of EN2 were measured in Cal27 and HN6 cells under four conditions. Cell proliferation, migration and invasion were significantly reduced following LINC00973 knockdown but restored by ectopic EN2 overexpression as gauged by CCK-8 ( i ), wound healing ( j ) and Transwell invasion assays ( k ). Scale bar: 50 μm. l The migration/invasion-related markers were determined by western blot in Cal27 and HN6 cells with/without LINC00973 knockdown coupled with/without EN2 overexpression. m 1 × 10 5 HN6 cells with LINC00973 or/and EN2 manipulations were injected submucosally into the floor of the mouth. Fourteen days post-injection, tumor masses and cervical lymph nodes were harvested. Representative tumor masses images and estimated tumor volumes were showed. Scale bar: 1 cm. Representative IHC staining of Ki67 and EN2 in xenograft tumors ( n ) and their quantification data were shown ( o ). Scale bar: 50 μm. p Representative IHC staining of CK5/6 in lymph nodes (left panel) from orthotopic tumor-bearing mice among four indicated groups were displayed. The percentage of lymph nodes metastasis were shown and compared (right panel). Fisher’s exact test. Data were presented as mean ± SD, ** P < 0.01, Student’s t test.

Article Snippet: After blocking with QuickBlock Blocking Buffer (#P0220, Beyotime, China), sections were incubated overnight at 4 °C with primary antibodies against CK5/6 (#MAB-0744, Maixin, China), Ki67 (#MAB-0672, Maixin, China), EN2 (1:100, #sc-293311, Santa Cruz, USA), FOSL1 (1:1000, #5281, Cell signaling, USA) or NOTCH1 (1:500, #20687-1-AP, Proteintech, China).

Techniques: Expressing, Knockdown, Control, RNA Expression, Migration, Over Expression, CCK-8 Assay, Western Blot, Injection, Immunohistochemistry

Journal: International Journal of Biological Sciences

Article Title: BMP2-induced Adam12 + Fibroblasts Dictate Wound-associated Skin Scarring and Fibrosis

doi: 10.7150/ijbs.123725

Figure Lengend Snippet: Adam12 + fibroblasts undergo dynamic expansion during skin wound healing and are essential for wound-associated skin scarring and fibrosis. A Schematic depicting wounding of Adam12-tdTomato mice for a temporally defined assessment of Adam12 + fibroblasts. B, C Flow cytometry analysis of tdTomato in Adam12-tdTomato mice on unwound and wounded back skin at day 0 post-wounding (0dpw), 3dpw, 7dpw, 10dpw and 14dpw. Error bars represent SD (n=5 wounds). D, E Immunofluorescence staining analysis for tdTomato in C57BL/6J mice on unwound and wounded back skin at 0dpw, 3dpw, 7dpw, 10dpw and 14dpw. Error bars represent SD (n=5 wounds). F Schematic depicting wounding of Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice and depletion of Adam12 + cells in vivo . G, H Flow cytometry analysis of tdTomato indicated that Adam12 + cells were efficiently ablated after DT administration. Error bars represent SD (n=6 wounds). I, J Representative photographic images of wounds at 0 dpw and 14 dpw in both PBS and DT-treated wounds of Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice. Error bars represent SD (n=6 wounds). K, L Masson staining on wound tissues of DT treated mice and control mice at 14dpw. Error bars represent SD (n=6 wounds). Scale bar = 100μm. M-P, Immunofluorescence staining analysis for Collagen I and Collagen III on wound tissues of DT treated mice and control mice at 14dpw. Error bars represent SD (n=6 wounds). Scale bar = 100μm. One-way ANOVA test was used to determine statistical significance in C and E . Two-tailed Student's unpaired t-test was used to determine statistical significance in H, J, L, N and P .

Article Snippet: Adam12-tdTomato mice and Adam12 CreERT2 mice were constructed by Cyagen Company (Suzhou, China), in which the ATG start codon of Adam12 was replaced by Kozak-tdTomato-rBG pA or CreERT2-rBG pA. Postn fl/fl , Krt5 CreERT2 and R26 LSL-tdTomato mice were obtained from Cyagen Company (Suzhou, China).

Techniques: Flow Cytometry, Immunofluorescence, Staining, In Vivo, Control, Two Tailed Test

Journal: International Journal of Biological Sciences

Article Title: BMP2-induced Adam12 + Fibroblasts Dictate Wound-associated Skin Scarring and Fibrosis

doi: 10.7150/ijbs.123725

Figure Lengend Snippet: Periostin mediates the pro-fibrogenic activity of Adam12 + fibroblasts during skin scarring. A Schematic depicting wounding and tamoxifen induction of Adam12 CreERT2 ;Postn fl/fl mice for Adam12 + cells-specific Postn deletion. B, C Immunofluorescence staining analysis for periostin on wound tissues of Adam12 CreERT2 ;Postn +/+ , Adam12 CreERT2 ;Postn fl/+ , and Adam12 CreERT2 ;Postn fl/fl mice at 14dpw. Error bars represent SD (n=8-16 wounds). Scale bar = 100μm. D, E Masson staining on wound tissues of Adam12 CreERT2 ;Postn +/+ , Adam12 CreERT2 ;Postn fl/+ , and Adam12 CreERT2 ;Postn fl/fl mice at 14dpw. Error bars represent SD (n=8-16 wounds). Scale bar = 100μm. F Schematic depicting wounding, 2mg tamoxifen induction, 200ng DT injection and 1μg rmPOSTN (rmPN) injection of Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice. G, H Immunofluorescence staining analysis for periostin on wound tissues of PBS group and DT group Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice at 14dpw. Error bars represent SD (n=5 wounds). Scale bar = 100μm. I, J Representative photographic images of wound tissues of PBS group, DT group and DT+rmPOSTN group Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice at 0dpw and 14dpw. Error bars represent SD (n=5 wounds). K, L Masson staining on wound tissues of PBS group, DT group and DT+rmPOSTN group Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice at 14dpw. Error bars represent SD (n=5 wounds). Scale bar = 100μm. One-way ANOVA test was used to determine statistical significance in C, E, J and L . Two-tailed Student's unpaired t-test was used to determine statistical significance in H .

Article Snippet: Adam12-tdTomato mice and Adam12 CreERT2 mice were constructed by Cyagen Company (Suzhou, China), in which the ATG start codon of Adam12 was replaced by Kozak-tdTomato-rBG pA or CreERT2-rBG pA. Postn fl/fl , Krt5 CreERT2 and R26 LSL-tdTomato mice were obtained from Cyagen Company (Suzhou, China).

Techniques: Activity Assay, Immunofluorescence, Staining, Injection, Two Tailed Test

Journal: International Journal of Biological Sciences

Article Title: BMP2-induced Adam12 + Fibroblasts Dictate Wound-associated Skin Scarring and Fibrosis

doi: 10.7150/ijbs.123725

Figure Lengend Snippet: Adam12 + fibroblasts predominantly derive from resident fibroblasts in unwounded skin. A, B Immunofluorescence staining analysis for BMP2 in C57BL/6J mice on unwound and wounded back skin at 0dpw, 3dpw, 7dpw, 10dpw and 14dpw. Error bars represent SD (n=5 wounds). Scale bar = 100μm. C Schematic depicting wounding, vehicle injection and 20μg BMP2 inhibitor LDN-193189 2HCl injection of Adam12-tdTomato mice. D, E Flow cytometry analysis of tdTomato on vehicle injection or LDN-193189 2HCl injection wounds of Adam12-tdTomato mice. Error bars represent SD (n=5 wounds). F Schematic depicting tamoxifen induction and wounding of Marker CreERT2 ; R26 LSL-tdTomato mice for lineage tracing. G, H Immunofluorescence staining analysis for tdTomato and Adam12 on wounds of Pdgfra CreERT2 ; R26 LSL-tdTomato , Sm22 CreERT2 ; R26 LSL-tdTomato and Krt5 CreERT2 ; R26 LSL-tdTomato mice at 10dpw. Error bars represent SD (n=8 wounds for Pdgfra CreERT2 ; R26 LSL-tdTomato mice. n=4 wounds for Sm22 CreERT2 ; R26 LSL-tdTomato and Krt5 CreERT2 ; R26 LSL-tdTomato mice). Scale bar = 100μm. Two-tailed Student's paired t-test was used to determine statistical significance in E . One-way ANOVA test was used to determine statistical significance in B and H .

Article Snippet: Adam12-tdTomato mice and Adam12 CreERT2 mice were constructed by Cyagen Company (Suzhou, China), in which the ATG start codon of Adam12 was replaced by Kozak-tdTomato-rBG pA or CreERT2-rBG pA. Postn fl/fl , Krt5 CreERT2 and R26 LSL-tdTomato mice were obtained from Cyagen Company (Suzhou, China).

Techniques: Immunofluorescence, Staining, Injection, Flow Cytometry, Marker, Two Tailed Test

Journal: International Journal of Biological Sciences

Article Title: BMP2-induced Adam12 + Fibroblasts Dictate Wound-associated Skin Scarring and Fibrosis

doi: 10.7150/ijbs.123725

Figure Lengend Snippet: BMP2 signaling is required for the lineage commitment of resident dermal fibroblasts into pro-fibrogenic Adam12 + fibroblasts. A Schematic depicting wounding and tamoxifen induction of Pdgfra CreERT2 ;Bmpr2 fl/fl mice for fibroblasts-specific Bmpr2 deletion. B, C Immunofluorescence staining analysis for Adam12 on wound tissues of Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ , and Pdgfra CreERT2 ;Bmpr2 fl/fl mice at 14dpw. Error bars represent SD (n=6 wounds for Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ and n=8 wounds for Pdgfra CreERT2 ;Bmpr2 fl/fl ). Scale bar = 100μm. D, E Representative photographic images of wound tissues of Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ , and Pdgfra CreERT2 ;Bmpr2 fl/fl mice at 0dpw and 14dpw. Error bars represent SD (n=6 wounds for Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ and n=8 wounds for Pdgfra CreERT2 ;Bmpr2 fl/fl ). F, G Masson staining on wound tissues of Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ , and Pdgfra CreERT2 ;Bmpr2 fl/fl mice at 14dpw. Error bars represent SD (n=6 wounds for Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ and n=8 wounds for Pdgfra CreERT2 ;Bmpr2 fl/fl ). Scale bar = 100μm. H, I Immunofluorescence staining analysis for periostin on wound tissues of Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ , and Pdgfra CreERT2 ;Bmpr2 fl/fl mice at 14dpw. Error bars represent SD (n=6 wounds for Pdgfra CreERT2 ;Bmpr2 +/+ , Pdgfra CreERT2 ;Bmpr2 fl/+ and n=8 wounds for Pdgfra CreERT2 ;Bmpr2 fl/fl ). Scale bar = 100μm. One-way ANOVA test was used to determine statistical significance in C, E, G and I .

Article Snippet: Adam12-tdTomato mice and Adam12 CreERT2 mice were constructed by Cyagen Company (Suzhou, China), in which the ATG start codon of Adam12 was replaced by Kozak-tdTomato-rBG pA or CreERT2-rBG pA. Postn fl/fl , Krt5 CreERT2 and R26 LSL-tdTomato mice were obtained from Cyagen Company (Suzhou, China).

Techniques: Immunofluorescence, Staining

Journal: International Journal of Biological Sciences

Article Title: BMP2-induced Adam12 + Fibroblasts Dictate Wound-associated Skin Scarring and Fibrosis

doi: 10.7150/ijbs.123725

Figure Lengend Snippet: Augmentation of BMP2 signaling drives expansion of Adam12 + fibroblasts and exacerbates skin scarring. A-F, Immunofluorescence staining analysis for BMP2, ADAM12 and Periostin in normal scar, hypertrophic scar (HTS) and keloid. Error bars represent SD (n=8). Scale bar = 100μm. G The correlation analyses between the expression of BMP and ADAM12. The range between the two dashed lines represents the 95% confidence interval. H The correlation analyses between the expression of ADAM12 and periostin. The range between the two dashed lines represents the 95% confidence interval. I Schematic depicting wounding, 2mg tamoxifen induction, 200ng DT injection and 25ng BMP2 injection of Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice. J, K Flow cytometry analysis of tdTomato on vehicle+PBS injection, BMP2+PBS injection and BMP2+DT injection wounds of Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice. Error bars represent SD (n=6 wounds). L, M Representative photographic images of wound tissues of vehicle+PBS group, BMP2+PBS group and BMP2+DT group Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice at 0dpw and 14dpw. Error bars represent SD (n=6 wounds). N, O Masson staining on vehicle+PBS injection, BMP2+PBS injection and BMP2+DT injection wounds of Adam12 CreERT2 ; R26 LSL-tdTomato-2A-DTR mice. Error bars represent SD (n=6 wounds). Scale bar = 100μm. N Schematic illustration showing that abnormal high-expressed BMP2 in healed wound may lead to pathologic scars. One-way ANOVA test was used to determine statistical significance in B, D, F, K, M and O . Pearson correlation was used to measures the strength and direction of a linear relationship between two variables in G and H .

Article Snippet: Adam12-tdTomato mice and Adam12 CreERT2 mice were constructed by Cyagen Company (Suzhou, China), in which the ATG start codon of Adam12 was replaced by Kozak-tdTomato-rBG pA or CreERT2-rBG pA. Postn fl/fl , Krt5 CreERT2 and R26 LSL-tdTomato mice were obtained from Cyagen Company (Suzhou, China).

Techniques: Immunofluorescence, Staining, Expressing, Injection, Flow Cytometry