Journal: Biochemistry

Article Title: Revealing the Specificity of a Range of Antimicrobial Peptides in Lipid Nanodiscs by Native Mass Spectrometry

doi: 10.1021/acs.biochem.0c00335

Figure Lengend Snippet: Schematic showing experimental design and KR12 incorporation into nanodiscs. (A) Nanodiscs were mixed with KR12 at different molar ratios from 0–18:1 KR12:nanodisc and ionized by nano-ESI. (B) Mass spectra were obtained and (C) deconvolved using UniDec. (D) Normalized mass defects were used to quantify how many peptides were incorporated into the nanodiscs and relative intensities were extracted to show the number of peptides incorporated at different molar ratios with (E) DMPG nanodiscs.

Article Snippet: KR12 peptide was purchased from Bachem (Torrance, CA).

Techniques:

Journal: Biochemistry

Article Title: Revealing the Specificity of a Range of Antimicrobial Peptides in Lipid Nanodiscs by Native Mass Spectrometry

doi: 10.1021/acs.biochem.0c00335

Figure Lengend Snippet: LL37 (A, C) and KR12 (B, D) incorporation in DMPC (A, B) and DMPG (C, D) nanodiscs. Native mass spectra of (A) LL37 and (B) KR12 added to DMPC nanodiscs at concentrations from 0–18:1 peptide:nanodisc show breakdown of the nanodisc by LL37 and no effects of KR12. Small amounts of three KR12 incorporated in B are not statistically significant and are artifacts caused by similar mass defects to empty nanodiscs. Insets show the relative abundance of different stoichiometries of incorporated peptides. The relative abundances of (C) LL37 and (D) KR12 incorporated into DMPG nanodiscs show broader distributions with KR12. Data for LL37 is from Walker et al. (2019),12 and D is taken from Figure 1. The full sequence of LL37 is shown at the top with the KR12 truncation highlighted in red.

Article Snippet: KR12 peptide was purchased from Bachem (Torrance, CA).

Techniques: Sequencing

Journal: Biochemistry

Article Title: Revealing the Specificity of a Range of Antimicrobial Peptides in Lipid Nanodiscs by Native Mass Spectrometry

doi: 10.1021/acs.biochem.0c00335

Figure Lengend Snippet: Summary of AMP lipid and complex specificity. Structures were generated using PDB files: 1MAG (gramicidin A), 1G89 (indolicidin), 2NA3 (KR12), 1LFC (LacB), 5NNT (LL37), 2MAG (Mag2), 6DST (melittin), 2MAA (TempA), 6GS5 (TempL). Residues are colored as either nonpolar (white), polar (green), cationic (blue), or anionic (red). Peptide sequences are provided in Table S1.

Article Snippet: KR12 peptide was purchased from Bachem (Torrance, CA).

Techniques: Generated

Journal: Molecules

Article Title: Interactions of Laurylated and Myristoylated KR12 Fragment of the LL37 Peptide with Polyoxidovanadates

doi: 10.3390/molecules30071589

Figure Lengend Snippet: Thermodynamic parameters of binding interactions of C12-KR12 and C14-KR12 with [V 10 O 28 ] 6− in the 50 mM sodium cacodylate buffer with a pH of 5, at 298.15 K (standard deviation values in parentheses).

Article Snippet: The peptides C12-KR12 (dodecanoic acid-KRIVQRIKDFLR-NH 2 ) and C14-KR12 (tetradecanoic acid-KRIVQRIKDFLR-NH 2 ) were synthesized manually by the solid-phase method using 9-fluorenylmethoxycarbonyl (Fmoc) chemistry on resin modified by a Rink amide linker resin with loading of 1.0 mmol/g (Orpegen Peptide Chemicals GmbH, Heidelberg, Germany) according to the procedure described in our previous work [ ].

Techniques: Binding Assay, Standard Deviation

Journal: Molecules

Article Title: Interactions of Laurylated and Myristoylated KR12 Fragment of the LL37 Peptide with Polyoxidovanadates

doi: 10.3390/molecules30071589

Figure Lengend Snippet: Temperature-dependent α-helical content of C12-KR12 and C14-KR12 in 50 mM sodium cacodylate buffer (pH 5) with and without [V 10 O 28 ] 6− .

Article Snippet: The peptides C12-KR12 (dodecanoic acid-KRIVQRIKDFLR-NH 2 ) and C14-KR12 (tetradecanoic acid-KRIVQRIKDFLR-NH 2 ) were synthesized manually by the solid-phase method using 9-fluorenylmethoxycarbonyl (Fmoc) chemistry on resin modified by a Rink amide linker resin with loading of 1.0 mmol/g (Orpegen Peptide Chemicals GmbH, Heidelberg, Germany) according to the procedure described in our previous work [ ].

Techniques: