klrg1 Search Results


pe cy7 anti klrg1  (Miltenyi Biotec)
93
Miltenyi Biotec pe cy7 anti klrg1
Pe Cy7 Anti Klrg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/KLRG1+Antibody%2C+anti-human%2C+REAfinity/pmc08183213-54-37-40
Average 93 stars, based on 1 article reviews
pe cy7 anti klrg1 - by Bioz Stars, 2026-10
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mouse monoclonal antibody anti cxcr2  (R&D Systems)
93
R&D Systems mouse monoclonal antibody anti cxcr2
Mouse Monoclonal Antibody Anti Cxcr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/Mouse+KLRG1+Antibody/pm24388382-83-11-15
Average 93 stars, based on 1 article reviews
mouse monoclonal antibody anti cxcr2 - by Bioz Stars, 2026-10
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klrg1 violet fluor 450  (Cytek Biosciences)
93
Cytek Biosciences klrg1 violet fluor 450
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Klrg1 Violet Fluor 450, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/violetFluor+450+Anti-Mouse+KLRG1/pmc10319879-243-91-98
Average 93 stars, based on 1 article reviews
klrg1 violet fluor 450 - by Bioz Stars, 2026-10
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anti human  (R&D Systems)
93
R&D Systems anti human
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Anti Human, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/Human+KLRG1+Antibody/pmc05321027-304-88-93
Average 93 stars, based on 1 article reviews
anti human - by Bioz Stars, 2026-10
93/100 stars
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rabbit anti klrg1  (Biorbyt)
85
Biorbyt rabbit anti klrg1
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Rabbit Anti Klrg1, supplied by Biorbyt, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/KLRG1+antibody+pair/pmc05074366-234-19-21
Average 85 stars, based on 1 article reviews
rabbit anti klrg1 - by Bioz Stars, 2026-10
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klrg1  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology klrg1
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Klrg1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/KLRG1+Antibody/pmc05940260-229-57-68
Average 92 stars, based on 1 article reviews
klrg1 - by Bioz Stars, 2026-10
92/100 stars
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anti klrg1  (R&D Systems)
92
R&D Systems anti klrg1
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Anti Klrg1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/KLRG1+Antibody+(8E11)/10__2147_slash_ijn__s268282-77-122-123
Average 92 stars, based on 1 article reviews
anti klrg1 - by Bioz Stars, 2026-10
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anti v maf musculoaponeurotic fibrosarcoma oncogene homolog a anti mafa  (Proteintech)
91
Proteintech anti v maf musculoaponeurotic fibrosarcoma oncogene homolog a anti mafa
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Anti V Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A Anti Mafa, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/KLRG1+Antibody/pm31649162-62-34-48
Average 91 stars, based on 1 article reviews
anti v maf musculoaponeurotic fibrosarcoma oncogene homolog a anti mafa - by Bioz Stars, 2026-10
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anti mouse klrg1 2f1 klrg1 bioxcell  (Bio X Cell)
92
Bio X Cell anti mouse klrg1 2f1 klrg1 bioxcell
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Anti Mouse Klrg1 2f1 Klrg1 Bioxcell, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/InVivoMAb+anti-mouse+human+KLRG-1/pm37236195-277-60-63
Average 92 stars, based on 1 article reviews
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fitc klrg1  (Cytek Biosciences)
90
Cytek Biosciences fitc klrg1
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Fitc Klrg1, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/FITC+Anti-Mouse+KLRG1/pm39345351-87-23-25
Average 90 stars, based on 1 article reviews
fitc klrg1 - by Bioz Stars, 2026-10
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nucleofector solution  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology nucleofector solution
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Nucleofector Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/KLRG1+siRNA/pmc05027915-144-12-25
Average 90 stars, based on 1 article reviews
nucleofector solution - by Bioz Stars, 2026-10
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inc migg2c none ihc mab70293 unknown r d systems rabbit igg none flow  (R&D Systems)
93
R&D Systems inc migg2c none ihc mab70293 unknown r d systems rabbit igg none flow
a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of <t>KLRG1.</t> m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.
Inc Migg2c None Ihc Mab70293 Unknown R D Systems Rabbit Igg None Flow, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/klrg1/Human+KLRG1+Antibody/pmc11145167__ccr___23___3504_supplementary_data_1_suppds1-2-46-52
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Image Search Results


a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of KLRG1. m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: T cell receptor signaling strength establishes the chemotactic properties of effector CD8 + T cells that control tissue-residency

doi: 10.1038/s41467-023-39592-1

Figure Lengend Snippet: a Naïve IFNγ-YFP P14 CD8 + T cells were transferred into B6 mice and infected on the ear skin with VacV-GP33. YFP expression in the indicated organs was quantified by flow cytometry on day 7 post infection. b Quantification of ( a ) at the indicated timepoints after infection; n = 3 per tissue per time point. c Quantification of the total number and YFP + P14 CD8 + T cells over time; n = 3. d Same as ( a ) except CD8β antibody was injected intravenously before sacrifice to distinguish YFP-P14 CD8 + T cells within the skin from those in the vasculature. e Quantification of ( d ); n = 3. f Same as ( a ) except YFP expression was quantified by P14 CD8 + T cells in the dermis and epidermis on day 7 post infection. g Quantification of ( f ); n = 4. h Experiment design for ( i – r ). i Representative flow plots depicting the total number of P14 CD8 + T cells and YFP expression by IFNγ-YFP P14 CD8 + T in the skin on day 7 post infection. j Quantification of the total number of P14 CD8 + T cells in ( i ); n = 12. k Quantification of YFP expression in ( i ); n = 12. l Representative histograms depicting the expression of KLRG1. m Quantification of ( l ); n = 3. n Representative flow plots depicting the expression of YFP and KLRG1. o Quantification of ( n ); n = 3. p Quantification of the total number of IFNγ-YFP P14 CD8 + T cells in the skin on day 40 post infection; n = 5. q Representative flow plots depicting the expression of CD69 and CD103 on day 40 post infection. r Quantification of ( q ); n = 5. Data shown are mean ± SD and representative of 2 or more independent experiments except (j,k) which are cumulative data from 3 independent experiments. Statistical significance was calculated using a paired two-sided t-test ( e , g , k , o , p , r ) or one-way ANOVA followed by Tukey’s multiple comparisons test ( m ). Source data are provided as a Source Data file.

Article Snippet: The following antibodies along with appropriate isotype controls were used in this study: CD45.2 PE/Cyanin-7, Pacific Blue or APC (1:400; Clone 104; BioLegend; Cat# 109830, 109820 or 109814), CD8α Brilliant Violet 711, Pacific Blue, APC, or BUV395 (1:400; Clone 53-6.7; BioLegend; Cat# 100759, 100725, 100711, or BD Biosciences Cat# 563786), CD44 Pacific Blue (1:400; Clone IM7; BioLegend; Cat# 103020), Thy1.1 PerCP/Cy5.5, Brilliant Violet 711, or Pacific Blue (1:1000; Clone OX7; BioLegend; Cat# 109004, 202539 or 202522), Thy1.2 Brilliant Violet 605 or Pacific Blue (1:1000; clone 53-2.1; BioLegend; Cat# 140318 or 140306), KLRG1 Violet Fluor 450 (1:200; Clone 2F1; Tonbo; Cat# 75-5893-U100), CD8β PerCP/Cy5.5 (Clone YST156.7.7; BioLegend; Cat# 126609), CD69 FITC or Pacific Blue (1:100; Clone H1.2FE, BioLegend; Cat# 104506 or 104523), CD103 PE (1:200; Clone 2-E7; BioLegend; Cat# 121406), IFNγ APC (1:200; Clone XMG1.2; BioLegend; Cat# 505810), PD-1 PE (1:100; Clone 29F1.1a12; BioLegend; Cat# 135206), ICOS FITC or PE (1:200; Clone 7E.17G9, eBioscience; Cat# 11-9942-82 or 12-9942-82), CXCR6 APC (1:300; Clone SA051D1, BioLegend; Cat# 151106), Blimp1 PE (1:200; Clone 5-E7, BioLegend; Cat# 150006), TCF-1 PE (1:200; Clone S33-966; BD Bioscience; Cat# 564217), Ki-67 PE/Cyanin7 (1:200; Clone 16A8, BioLegend; Cat# 652425), CD62L PE or APC (1:400; Clone MEL-14; BioLegend; Cat# 104408 or 104412), CD25 PE (1:200; Clone PC61; BioLegend; Cat# 102007) and Viability-Ghost Dye Red 780 (1:1000; Tonbo; Cat #13-0865-T100).

Techniques: Infection, Expressing, Flow Cytometry, Injection