Journal: Iranian Journal of Pathology

Article Title: Expression of Human Kallikreins 4, 8, 10, 11 and 13 in Pleomorphic Adenomas and Mucoepidermoid Carcinomas

doi:

Figure Lengend Snippet: Staining in ductal cells and non-ductal cells and total scores for immunostaining of pleomorphic adenomafor human kallikrein 11 (Average staining ± SD)

Article Snippet: Appropriate positive and negative (tissue slides with primary antibodies omitted) controls were used (Human KLK-8/Kallikrein-8 Human Cells Transfected Lysate (positive control), Human KLK-4/ Kallikrein-4 Human Cells Transfected Lysate (positive control), Kallikrein 13 transfected 293T cell lysat, Lysate from 293T cells transfected with Kallikrein 10 and KLK11 / Kallikrein-11 Human Cells Transfected Lysate) (Sino Biological Inc, China and Asia Chemi Tab Co, Tehran, Iran).

Techniques: Staining, Immunostaining

Journal: Experimental and therapeutic medicine

Article Title: Knockdown of KLK11 inhibits cell proliferation and increases oxaliplatin sensitivity in human colorectal cancer.

doi: 10.3892/etm.2016.3723

Figure Lengend Snippet: Figure 1. Establishment of cell lines that stably knockdown KLK11. Expression of (A) KLK11 mRNA and (B) KLK11 protein in LOVO cells and (C) KLK11 mRNA and (D) KLK11 protein in HCT‑8 cells. mRNA levels were detected by reverse transcription‑quantitative polymerase chain reaction, and protein levels by western blot analysis. β‑actin was used as a loading control. *P<0.05, **P<0.01 vs. SCR. KLK11, kallikrein 11; SCR, short hairpin RNA control.

Article Snippet: Blocking was performed at room temperature using Tris‐buffered saline with 0.1% Tween‐20 (TBST; J&K Chemical Ltd., Shanghai, China) containing 5% non-fat milk for 1 h. The membrane was then incubated with primary mouse monoclonal KLK11 antibody (sc‐20387; 1:500) and rabbit polyclonal β-actin (sc‐47778; 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), Bcl‐2 (#2872; 1:1,000) and Bax (#2772; 1:1,000) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA) in TBST at 4 ̊C overnight and with the appropriate horseradish peroxidase-conjugated secondary antibody (CW0103M; 1:3000; CWbiotech, Beijing, China) for 1 h at room temperature.

Techniques: Stable Transfection, Knockdown, Expressing, Polymerase Chain Reaction, Western Blot, Control, shRNA

Journal: Experimental and therapeutic medicine

Article Title: Knockdown of KLK11 inhibits cell proliferation and increases oxaliplatin sensitivity in human colorectal cancer.

doi: 10.3892/etm.2016.3723

Figure Lengend Snippet: Figure 2. KLK11 shRNA inhibited cell proliferation and enhanced apoptosis. Proliferation levels of (A) LOVO and (B) HCT‑8 cells after KLK11 silencing analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. (C) Flow cytometric analysis of apoptosis in LOVO and HCT‑8 cells trans fected with SCR or shKLK11. (D) Quantification of the apoptotic cells. *P<0.05, **P<0.01 vs. SCR. SCR, short hairpin RNA control; KLK11, kallikrein 11.

Article Snippet: Blocking was performed at room temperature using Tris‐buffered saline with 0.1% Tween‐20 (TBST; J&K Chemical Ltd., Shanghai, China) containing 5% non-fat milk for 1 h. The membrane was then incubated with primary mouse monoclonal KLK11 antibody (sc‐20387; 1:500) and rabbit polyclonal β-actin (sc‐47778; 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), Bcl‐2 (#2872; 1:1,000) and Bax (#2772; 1:1,000) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA) in TBST at 4 ̊C overnight and with the appropriate horseradish peroxidase-conjugated secondary antibody (CW0103M; 1:3000; CWbiotech, Beijing, China) for 1 h at room temperature.

Techniques: shRNA, Control

Journal: Experimental and therapeutic medicine

Article Title: Knockdown of KLK11 inhibits cell proliferation and increases oxaliplatin sensitivity in human colorectal cancer.

doi: 10.3892/etm.2016.3723

Figure Lengend Snippet: Figure 3. Effect of KLK11 silencing on the apoptosis signaling pathway. (A) Western blot analysis of Bcl‑2 and Bax protein expression in LOVO and HCT‑8 cells transfected with SCR or shKLK11. β‑actin was used as a loading control. (B) Relative caspase‑3 activity was determined in LOVO and HCT‑8 cells transfected with SCR or shKLK11. **P<0.01 vs. SCR. Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated protein X; SCR, short hairpin RNA control; KLK11, kallikrein 11.

Article Snippet: Blocking was performed at room temperature using Tris‐buffered saline with 0.1% Tween‐20 (TBST; J&K Chemical Ltd., Shanghai, China) containing 5% non-fat milk for 1 h. The membrane was then incubated with primary mouse monoclonal KLK11 antibody (sc‐20387; 1:500) and rabbit polyclonal β-actin (sc‐47778; 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), Bcl‐2 (#2872; 1:1,000) and Bax (#2772; 1:1,000) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA) in TBST at 4 ̊C overnight and with the appropriate horseradish peroxidase-conjugated secondary antibody (CW0103M; 1:3000; CWbiotech, Beijing, China) for 1 h at room temperature.

Techniques: Western Blot, Expressing, Transfection, Control, Activity Assay, shRNA

Journal: Experimental and therapeutic medicine

Article Title: Knockdown of KLK11 inhibits cell proliferation and increases oxaliplatin sensitivity in human colorectal cancer.

doi: 10.3892/etm.2016.3723

Figure Lengend Snippet: Figure 4. KLK11 silencing modulates the sensitivity to L‑OHP. Viability of KLK11 knockdown (A) LOVO and (B) HCT‑8 cells treated with the indicated concentrations of L‑OHP for 24 h. (C) The effect of L‑OHP on apoptotic levels of the indicated cells analyzed by flow cytometry. (D) Quantification of the apoptotic cells. *P<0.05, **P<0.01 vs. control. KLK11, kallikrein 11; L-OHP, oxaliplatin.

Article Snippet: Blocking was performed at room temperature using Tris‐buffered saline with 0.1% Tween‐20 (TBST; J&K Chemical Ltd., Shanghai, China) containing 5% non-fat milk for 1 h. The membrane was then incubated with primary mouse monoclonal KLK11 antibody (sc‐20387; 1:500) and rabbit polyclonal β-actin (sc‐47778; 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), Bcl‐2 (#2872; 1:1,000) and Bax (#2772; 1:1,000) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA) in TBST at 4 ̊C overnight and with the appropriate horseradish peroxidase-conjugated secondary antibody (CW0103M; 1:3000; CWbiotech, Beijing, China) for 1 h at room temperature.

Techniques: Knockdown, Flow Cytometry, Control

Journal: Arthritis Research & Therapy

Article Title: A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients

doi: 10.1186/ar2002

Figure Lengend Snippet: Time-fold up- or down-expression of genes analyzed by real time PCR in SSc-MVECs versus N-MVECs

Article Snippet: VIC-labeled human glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #4326317E) and FAM-labeled human IL8 (#Hs00174103_m1), urokinase type plasminogen activator (PLAU; #Hs00705898_s1) KLK9 (#Hs00705898_s1), KLK11 (#Hs00170182_m1 and Hs00374668_m1), KLK12 (#Hs00377603_m1), PTX3 (#Hs00173615_m1), PLXNB1 (#Hs00182227_m1), DSG2 (#Hs00170071_m1), and connective tissue growth factor (CTGF; #Hs00170014_m1) TaqMan pre-developed assays (Applied Biosystems, Foster City, CA, USA) were used.

Techniques: Real-time Polymerase Chain Reaction

Journal: Arthritis Research & Therapy

Article Title: A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients

doi: 10.1186/ar2002

Figure Lengend Snippet: List of all the Gene Ontology significant terms with more than two annotated genes on the array (N > 2)

Article Snippet: VIC-labeled human glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #4326317E) and FAM-labeled human IL8 (#Hs00174103_m1), urokinase type plasminogen activator (PLAU; #Hs00705898_s1) KLK9 (#Hs00705898_s1), KLK11 (#Hs00170182_m1 and Hs00374668_m1), KLK12 (#Hs00377603_m1), PTX3 (#Hs00173615_m1), PLXNB1 (#Hs00182227_m1), DSG2 (#Hs00170071_m1), and connective tissue growth factor (CTGF; #Hs00170014_m1) TaqMan pre-developed assays (Applied Biosystems, Foster City, CA, USA) were used.

Techniques: Chemotaxis Assay, Transduction, Coagulation

Journal: Arthritis Research & Therapy

Article Title: A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients

doi: 10.1186/ar2002

Figure Lengend Snippet: Class distribution of differentially expressed genes with a log odds ratio >1

Article Snippet: VIC-labeled human glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #4326317E) and FAM-labeled human IL8 (#Hs00174103_m1), urokinase type plasminogen activator (PLAU; #Hs00705898_s1) KLK9 (#Hs00705898_s1), KLK11 (#Hs00170182_m1 and Hs00374668_m1), KLK12 (#Hs00377603_m1), PTX3 (#Hs00173615_m1), PLXNB1 (#Hs00182227_m1), DSG2 (#Hs00170071_m1), and connective tissue growth factor (CTGF; #Hs00170014_m1) TaqMan pre-developed assays (Applied Biosystems, Foster City, CA, USA) were used.

Techniques: Migration, Binding Assay, Transduction

Journal: Arthritis Research & Therapy

Article Title: A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients

doi: 10.1186/ar2002

Figure Lengend Snippet: Immunohistochemical evidence of differential expression of tissue kallikrein (KLK)9, KLK11, KLK12, and DSG2 in microvascular endothelial cells (MVECs) from normal subjects and patients with systemic sclerosis (SSc). Each panel is representative of the pattern of immune staining with each antibody obtained for the six normal and six SSc patients subjected to skin biopsies from which MVECs were prepared. The inset in the micrograph of KLK12 shows negative staining using isotype control rabbit IgG (the isotype used for KLK9 and KLK12), while that shown in the micrograph of DSG shows negative staining for mouse IgG (the isotype used for KLK11 and DSG). Original magnification, 200×.

Article Snippet: VIC-labeled human glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #4326317E) and FAM-labeled human IL8 (#Hs00174103_m1), urokinase type plasminogen activator (PLAU; #Hs00705898_s1) KLK9 (#Hs00705898_s1), KLK11 (#Hs00170182_m1 and Hs00374668_m1), KLK12 (#Hs00377603_m1), PTX3 (#Hs00173615_m1), PLXNB1 (#Hs00182227_m1), DSG2 (#Hs00170071_m1), and connective tissue growth factor (CTGF; #Hs00170014_m1) TaqMan pre-developed assays (Applied Biosystems, Foster City, CA, USA) were used.

Techniques: Immunohistochemical staining, Expressing, Staining, Negative Staining

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: The expression of KLK11 is significantly lower in ESCC tissues. A and B. Indicated detection of KLK11 expression in dataset TCGA. C and D. Indicated KLK11 protein expression in 8 pairs of ESCC tissues and their para-carcinoma tissues by western blotting. KLK11 expression in ESCC tissues were significantly lower than those in the matched adjacent non-tumor tissues. E. KLK11 protein level was measured by immunohistochemical analysis in normal tissues. F. KLK11 protein level was measured by immunohistochemical analysis in ESCC tissues. Representative images are showed at 100 × and 200 × magnifications, respectively. G. KLK11 protein level in 100 ESCC tissues and matched normal tissues. Data were representative of three independent experiments (mean and SEM). *P < 0.05.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Expressing, Western Blot, Immunohistochemical staining

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: KLK11 expression was significantly associated with clinical characteristics

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: Summary of univariate and multivariate Cox regression analyses of overall survival

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: Low KLK11 expression was associated with poor prognosis in ESCC patients. ESCC samples from the tissue microarray were divided as two different groups, high KLK11 expression and low KLK11 expression group. Patients’ survival information was provided by the manufacture. Kaplan-Meier analysis of overall survival was done for the two group.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Expressing, Microarray

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: The expression of KLK11 is significantly lower in ESCC cell lines. QPCR (A) and western blotting (B) showed the expression of KLK11 in seven ESCC cell lines and HEEC. KLK11 knockdown efficiency was confirmed by qPCR (C) and western blotting (D) in ESCC cells. qPCR (E) and western blotting (F) confirmed the restoration of KLK11 expression in four groups of TE-1 cells. qPCR (G) and western blotting (H) confirmed the restoration of KLK11 expression in four groups of EC18 cells. Results were showed as mean ± SD (*P < 0.05) of triplicate determination from three independent experiments.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Expressing, Western Blot, Knockdown

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: KLK11 inhibited the proliferation of ESCC cells. A. KLK11 inhibited the proliferation of TE-1 cells by MTT assays. B. KLK11 inhibited the proliferation of EC18 cells by MTT assays. C. Colony formation assays showed that KLK11 overexpression inhibited TE-1 and EC18 cells proliferation. These effects were abolished by treatment with shKLK11. D. Flow cytometry analysis of cell cycle phase revealed a G0/G1 arrest induced by KLK11. Data were representative of three independent experiments (mean and SEM). *P < 0.05.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Over Expression, Flow Cytometry

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: KLK11 affected the expression of the related factors in Wnt/β-catenin pathway and cell cycle-mediated factors. A. The expression of p-GSK-3β/GSK-3β in EC18 and TE-1 cells after lentivirus vectors delivery in each group as measured by western blot. B. The TCF/LEF luciferase ratio reported the activity of Wnt/β-catenin pathway in the indicated cells. C. Expression of CDK4, cyclin D1, CDK6, p-Rb, Rb, β-catenin (cytosol), and β-catenin (nucleus) were determined in TE-1 and EC18 cells transfected with KLK11. KLK11 inhibited the proliferation of ESCC by regulating the expression of the above proteins. D. Analysis of Ki67, cyclin D1, CDK4, CDK6, and c-myc expression in KLK11-overexpression or KLK11-knockdown ESCC cells by qPCR. Data were representative of three independent experiments (mean and SEM). *P < 0.05.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Expressing, Western Blot, Luciferase, Activity Assay, Transfection, Over Expression, Knockdown

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: KLK11 down regulated the expression of Ki67 and cyclin D1 through deactivation of the Wnt/β-catenin signaling pathway. A, B. ESCC cells were pre-treated with XAV-939 for 24 h before harvesting, and the detection of Ki67, cyclin D1, c-myc, and expression levels were performed by qPCR. C, D. Treatment of EC18 and TE-1 cells with XAV-939, the expression of Ki67, cyclin D1, β-catenin (cytosol), and β-catenin (nucleus) were significantly inhibited. Data were representative of three independent experiments (mean and SEM). *P < 0.05.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: KLK11 suppressed ESCC cells proliferation through inhibiting the activity of the Wnt/β-catenin pathway. A, B. XAV-939 was added to TE-1 and EC18 cells transfected with shRNA, and the proliferation of TE-1 and EC18 cells was detected by MTT assays. C. XAV-939 was added to TE-1 and EC18 cells transfected with shRNA, and the proliferation of TE-1 and EC18 cells was detected by colony formation assays. D. The relationship between KLK11 and the Wnt/β-catenin pathway. Data were representative of three independent experiments (mean and SEM). *P < 0.05.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: Activity Assay, Transfection, shRNA

Journal: American Journal of Cancer Research

Article Title: KLK11 suppresses cellular proliferation via inhibition of Wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma

doi:

Figure Lengend Snippet: KLK11 inhibited the proliferation of ESCC in vivo. (A, B) Tumor xenograft volume (A) and tumor size (B) in shRNA-KLK11-treated nude mice were bigger than those in the scramble group. Tumor sizes in KLK11 overexpression nude mice were smaller than those in the vector group. (C) Analysis of Ki67, cyclin D1, and KLK11 expression in mice tumor tissues with KLK11 overexpression or KLK11 knockdown by qPCR. (D) Analysis of KLK11 and Cyclin D1 in xenografts by Immunohistochemistry. Data were representative of three independent experiments (mean and SEM). *P < 0.05.

Article Snippet: Three pairs of short hairpin RNA (shRNA) targeting KLK11 lentivirus vectors (shKLK11#1) and negative controls (scramble, NC) were specifically synthesized by Synbio Technologies (Suzhou, China).

Techniques: In Vivo, shRNA, Over Expression, Plasmid Preparation, Expressing, Knockdown, Immunohistochemistry