kit buffer Search Results


pbs blocking buffer  (LI-COR)
99
LI-COR pbs blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Pbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs blocking buffer/product/LI-COR
Average 99 stars, based on 1 article reviews
pbs blocking buffer - by Bioz Stars, 2026-05
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tnb 0607 kit naı̈ve cd8 t cell negative selection kit easysep  (Cytek Biosciences)
97
Cytek Biosciences tnb 0607 kit naı̈ve cd8 t cell negative selection kit easysep
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Tnb 0607 Kit Naı̈ve Cd8 T Cell Negative Selection Kit Easysep, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tnb 0607 kit naı̈ve cd8 t cell negative selection kit easysep/product/Cytek Biosciences
Average 97 stars, based on 1 article reviews
tnb 0607 kit naı̈ve cd8 t cell negative selection kit easysep - by Bioz Stars, 2026-05
97/100 stars
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flow cytometry fixation permeabilization buffer kit i  (R&D Systems)
94
R&D Systems flow cytometry fixation permeabilization buffer kit i
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Flow Cytometry Fixation Permeabilization Buffer Kit I, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flow cytometry fixation permeabilization buffer kit i/product/R&D Systems
Average 94 stars, based on 1 article reviews
flow cytometry fixation permeabilization buffer kit i - by Bioz Stars, 2026-05
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permeabilization buffer kit  (R&D Systems)
94
R&D Systems permeabilization buffer kit
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Permeabilization Buffer Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/permeabilization buffer kit/product/R&D Systems
Average 94 stars, based on 1 article reviews
permeabilization buffer kit - by Bioz Stars, 2026-05
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blocking buffer  (Rockland Immunochemicals)
93
Rockland Immunochemicals blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Blocking Buffer, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking buffer/product/Rockland Immunochemicals
Average 93 stars, based on 1 article reviews
blocking buffer - by Bioz Stars, 2026-05
93/100 stars
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intracellular fixation permeabilization buffer kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology intracellular fixation permeabilization buffer kit
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Intracellular Fixation Permeabilization Buffer Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intracellular fixation permeabilization buffer kit/product/Elabscience Biotechnology
Average 95 stars, based on 1 article reviews
intracellular fixation permeabilization buffer kit - by Bioz Stars, 2026-05
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transposition mix  (Illumina Inc)
97
Illumina Inc transposition mix
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Transposition Mix, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transposition mix/product/Illumina Inc
Average 97 stars, based on 1 article reviews
transposition mix - by Bioz Stars, 2026-05
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buffer large kit  (Illumina Inc)
97
Illumina Inc buffer large kit
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Buffer Large Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer large kit/product/Illumina Inc
Average 97 stars, based on 1 article reviews
buffer large kit - by Bioz Stars, 2026-05
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li cor blocking buffer  (LI-COR)
99
LI-COR li cor blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Li Cor Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/li cor blocking buffer/product/LI-COR
Average 99 stars, based on 1 article reviews
li cor blocking buffer - by Bioz Stars, 2026-05
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buffer kit  (Bio-Rad)
92
Bio-Rad buffer kit
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Buffer Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer kit/product/Bio-Rad
Average 92 stars, based on 1 article reviews
buffer kit - by Bioz Stars, 2026-05
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protein a g hp spintraptm buffer kit  (Danaher Inc)
91
Danaher Inc protein a g hp spintraptm buffer kit
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Protein A G Hp Spintraptm Buffer Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein a g hp spintraptm buffer kit/product/Danaher Inc
Average 91 stars, based on 1 article reviews
protein a g hp spintraptm buffer kit - by Bioz Stars, 2026-05
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1x foxp3 transcription factor fixation buffer  (R&D Systems)
92
R&D Systems 1x foxp3 transcription factor fixation buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
1x Foxp3 Transcription Factor Fixation Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x foxp3 transcription factor fixation buffer/product/R&D Systems
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Image Search Results


Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with PBS, lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total protein. Membranes were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .

Journal: Pharmaceutical Biology

Article Title: Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cells

doi: 10.1080/13880209.2026.2640678

Figure Lengend Snippet: Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with PBS, lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total protein. Membranes were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .

Article Snippet: Complete Mini Protease Inhibitor Cocktail Tablet was obtained from Roche; the BCA Protein Assay Kit from Thermo Fisher Scientific; 4–20% SDS-PAGE Gels from Bio-Rad; PVDF membranes from Merck; and Intercept TM (PBS) Blocking Buffer from LI-COR Biosciences.

Techniques: Clinical Proteomics, Concentration Assay, Control, Positive Control, Western Blot, Standard Deviation