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Image Search Results
Journal:
Article Title: Molecular Profiles of Mitogen Activated Protein Kinase Signaling Pathways in Orofacial Development
doi: 10.1002/bdra.20320
Figure Lengend Snippet: Immunoblot analysis of total and active p38 MAPK and SAPK/JNK in embryonic orofacial tissue on GDs 12, 13 and 14. A total of 60 µg of total protein lysate was separated by SDS-PAGE electrophoresis on 8–16% Tris-Glycine gels. A) Active, phosphorylated (upper panel) and total (lower panel) SAPK/JNK were analyzed by immunoblotting with specific antibodies. B) Active, phosphorylated (upper panel) and total (lower panel) p38 MAPK was analyzed by immunoblotting with specific antibodies. Minimal amounts of active, phosphorylated p38 were detected in embryonic orofacial tissue during GDs 12–14.
Article Snippet: Absorbance ratios measured for total RNA samples were from 1.98 to 2.10 in Tris-EDTA buffer solution (pH 7.5), indicating the quality of the samples.
Techniques: Western Blot, SDS Page, Electrophoresis