Journal: Oncology letters

Article Title: Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

doi: 10.3892/ol.2022.13265

Figure Lengend Snippet: Figure 1. CDH16 is downregulated in TC from three GEO datasets and there is no significant variation in copy number in TCGA dataset. The fold‑change, P‑value and underexpression gene ranks are based on Oncomine 4.5 analysis. Box plots showing CDH16 mRNA levels in patients with PTC in (A) He, (B) Vasko and (C) Giordano thyroid datasets from GEO. Box plot showing CDH16 mRNA levels in patients with (D) FTC and (E) ATC in the Giordano thyroid dataset. (F) Box plot showing CDH16 copy number in TCGA thyroid dataset. N, normal; CDH, cadherin; TC, thyroid carcinoma; GEO, Gene Expression Omnibus; TCGA, The Cancer Genome Atlas; PTC, papillary thyroid cancer; FTC, follicular thyroid cancer; ATC, anaplastic thyroid cancer.

Article Snippet: The membranes were incubated overnight at 4 ̊C with primary antibodies against CDH16 (1:1,000, cat. no. 15107‐1‐AP, ProteinTech Group, Inc.), DNA polymerase (POL)D1 (1:1,000, cat. no. 15646‐1‐AP, ProteinTech Group, Inc), minichromosome maintenance (MCM)6 (1:2,000, cat. no. 13347‐2‐AP; ProteinTech Group, Inc), claudin (CLDN)1 (1:1,500, cat. no. 13050‐1‐AP, ProteinTech Group, Inc), intercel‐ lular adhesion molecule (ICAM)1 (1:2,000, cat. no. 60299‐1‐Ig, ProteinTech Group, Inc), syndecan (SDC)4 (1:1,000, cat. no. 11820‐1‐AP, ProteinTech Group, Inc) or β‐actin (1:1,000, cat. no. TA‐09; OriGene Technologies, Inc.).

Techniques: Gene Expression

Journal: Oncology letters

Article Title: Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

doi: 10.3892/ol.2022.13265

Figure Lengend Snippet: Figure 2. CDH16 expression in 35 PTC tissue samples and PTC and follicular cell lines. Expression levels of CDH16 in 35 pairs of PTC and corresponding adjacent N tissue were evaluated by (A) RT‑qPCR and (B) immunohistochemical staining. Scale bar, 25 µm. ***P<0.001 vs. N. CDH16 expression in TC cell lines (BCPAP and TPC1) and the human thyroid follicular cell line Nthy‑ori3‑1 was detected by (C) RT‑qPCR and (D) western blotting. *P<0.05 vs. Nthy‑ori3‑1 cells. Data are presented as the mean ± SD of three independent experiments. N, normal; PTC, papillary thyroid cancer; CDH, cadherin; RT‑q, reverse transcription‑quantitative; TC, thyroid carcinoma.

Article Snippet: The membranes were incubated overnight at 4 ̊C with primary antibodies against CDH16 (1:1,000, cat. no. 15107‐1‐AP, ProteinTech Group, Inc.), DNA polymerase (POL)D1 (1:1,000, cat. no. 15646‐1‐AP, ProteinTech Group, Inc), minichromosome maintenance (MCM)6 (1:2,000, cat. no. 13347‐2‐AP; ProteinTech Group, Inc), claudin (CLDN)1 (1:1,500, cat. no. 13050‐1‐AP, ProteinTech Group, Inc), intercel‐ lular adhesion molecule (ICAM)1 (1:2,000, cat. no. 60299‐1‐Ig, ProteinTech Group, Inc), syndecan (SDC)4 (1:1,000, cat. no. 11820‐1‐AP, ProteinTech Group, Inc) or β‐actin (1:1,000, cat. no. TA‐09; OriGene Technologies, Inc.).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

Journal: Oncology letters

Article Title: Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

doi: 10.3892/ol.2022.13265

Figure Lengend Snippet: Figure 3. GSEA of co‑expressed genes with CDH16 in TC. (A) Volcano map of genes co‑expressed with CDH16 in TCGA samples, as evaluated by LinkedOmics. Red, positively correlated; green, negatively correlated. (B) RNA‑sequencing data from TCGA were analyzed by GSEA enrichment plots for KEGG_DNA_REPLICATION and KEGG_CELL_ADHENSION_MOLECULES_. KEGG, Kyoto Encyclopedia of Genes and Genomes; GSEA, Gene Set Enrichment Analysis; CDH, cadherin.

Article Snippet: The membranes were incubated overnight at 4 ̊C with primary antibodies against CDH16 (1:1,000, cat. no. 15107‐1‐AP, ProteinTech Group, Inc.), DNA polymerase (POL)D1 (1:1,000, cat. no. 15646‐1‐AP, ProteinTech Group, Inc), minichromosome maintenance (MCM)6 (1:2,000, cat. no. 13347‐2‐AP; ProteinTech Group, Inc), claudin (CLDN)1 (1:1,500, cat. no. 13050‐1‐AP, ProteinTech Group, Inc), intercel‐ lular adhesion molecule (ICAM)1 (1:2,000, cat. no. 60299‐1‐Ig, ProteinTech Group, Inc), syndecan (SDC)4 (1:1,000, cat. no. 11820‐1‐AP, ProteinTech Group, Inc) or β‐actin (1:1,000, cat. no. TA‐09; OriGene Technologies, Inc.).

Techniques:

Journal: Oncology letters

Article Title: Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

doi: 10.3892/ol.2022.13265

Figure Lengend Snippet: Figure 4. Correlation between POLD1, MCM6, CLDN1, ICAM1, SDC4 and CDH16 expression was analyzed using the LinkedOmics database. POL, poly‑ merase; MCM, minichromosome maintenance; CLDN, claudin; ICAM, intercellular adhesion molecule; SDC, syndecan; CDH, cadherin.

Article Snippet: The membranes were incubated overnight at 4 ̊C with primary antibodies against CDH16 (1:1,000, cat. no. 15107‐1‐AP, ProteinTech Group, Inc.), DNA polymerase (POL)D1 (1:1,000, cat. no. 15646‐1‐AP, ProteinTech Group, Inc), minichromosome maintenance (MCM)6 (1:2,000, cat. no. 13347‐2‐AP; ProteinTech Group, Inc), claudin (CLDN)1 (1:1,500, cat. no. 13050‐1‐AP, ProteinTech Group, Inc), intercel‐ lular adhesion molecule (ICAM)1 (1:2,000, cat. no. 60299‐1‐Ig, ProteinTech Group, Inc), syndecan (SDC)4 (1:1,000, cat. no. 11820‐1‐AP, ProteinTech Group, Inc) or β‐actin (1:1,000, cat. no. TA‐09; OriGene Technologies, Inc.).

Techniques: Expressing

Journal: Oncology letters

Article Title: Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

doi: 10.3892/ol.2022.13265

Figure Lengend Snippet: Figure 5. CDH16‑Overex inhibits proliferation and promotes apoptosis of BCPAP cells. (A) mRNA levels of CDH16 in CDH16‑Overex compared with control cells were examined by reverse transcription‑quantitative PCR. (B) Protein levels of CDH16 were assessed by western blotting. (C) Cell proliferation was evaluated by MTT assay and (D) apoptosis was evaluated by (E) flow cytometry following transfection. *P<0.05 and ***P<0.001 vs. negative control. Data are presented as the mean ± SD of three independent experiments. n=3; CDH16, cadherin; Overex, overexpression; OD, optical density.

Article Snippet: The membranes were incubated overnight at 4 ̊C with primary antibodies against CDH16 (1:1,000, cat. no. 15107‐1‐AP, ProteinTech Group, Inc.), DNA polymerase (POL)D1 (1:1,000, cat. no. 15646‐1‐AP, ProteinTech Group, Inc), minichromosome maintenance (MCM)6 (1:2,000, cat. no. 13347‐2‐AP; ProteinTech Group, Inc), claudin (CLDN)1 (1:1,500, cat. no. 13050‐1‐AP, ProteinTech Group, Inc), intercel‐ lular adhesion molecule (ICAM)1 (1:2,000, cat. no. 60299‐1‐Ig, ProteinTech Group, Inc), syndecan (SDC)4 (1:1,000, cat. no. 11820‐1‐AP, ProteinTech Group, Inc) or β‐actin (1:1,000, cat. no. TA‐09; OriGene Technologies, Inc.).

Techniques: Control, Western Blot, MTT Assay, Flow Cytometry, Transfection, Negative Control, Over Expression

Journal: Oncology letters

Article Title: Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

doi: 10.3892/ol.2022.13265

Figure Lengend Snippet: Figure 6. Overexpression of CDH16 inhibits migration and invasion of BCPAP cells. (A and B) Cell migration was evaluated by cell scratch test following CDH16 overexpression. Scale bar, 500 µm. (C) Migration ability of BCPAP cells following transfection was determined by (D) Transwell invasion assay. Scale bar, 100 µm. Data are presented as the mean ± SD of three independent experiments. *P<0.05 vs. negative control. CDH, cadherin; Overex, overexpression.

Article Snippet: The membranes were incubated overnight at 4 ̊C with primary antibodies against CDH16 (1:1,000, cat. no. 15107‐1‐AP, ProteinTech Group, Inc.), DNA polymerase (POL)D1 (1:1,000, cat. no. 15646‐1‐AP, ProteinTech Group, Inc), minichromosome maintenance (MCM)6 (1:2,000, cat. no. 13347‐2‐AP; ProteinTech Group, Inc), claudin (CLDN)1 (1:1,500, cat. no. 13050‐1‐AP, ProteinTech Group, Inc), intercel‐ lular adhesion molecule (ICAM)1 (1:2,000, cat. no. 60299‐1‐Ig, ProteinTech Group, Inc), syndecan (SDC)4 (1:1,000, cat. no. 11820‐1‐AP, ProteinTech Group, Inc) or β‐actin (1:1,000, cat. no. TA‐09; OriGene Technologies, Inc.).

Techniques: Over Expression, Migration, Transfection, Transwell Invasion Assay, Negative Control

Journal: Oncology letters

Article Title: Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

doi: 10.3892/ol.2022.13265

Figure Lengend Snippet: Figure 7. Overexpression of CDH16 downregulates DNA replication and cell adhesion pathway genes. (A) Representative western blots showing downregula‑ tion of POLD1, MCM6, CLDN1, ICAM1 and SDC4 following overexpression of CDH16. (B) Relative protein levels were quantified by densitometry of western blots (three replicates). Data are presented as the mean ± SD of three independent experiments. n=3. *P<0.05 vs. negative control. CDH16, cadherin; Overex, overexpression, POL, polymerase; MCM, minichromosome maintenance; CLDN, claudin; ICAM, intercellular adhesion molecule; SDC, syndecan.

Article Snippet: The membranes were incubated overnight at 4 ̊C with primary antibodies against CDH16 (1:1,000, cat. no. 15107‐1‐AP, ProteinTech Group, Inc.), DNA polymerase (POL)D1 (1:1,000, cat. no. 15646‐1‐AP, ProteinTech Group, Inc), minichromosome maintenance (MCM)6 (1:2,000, cat. no. 13347‐2‐AP; ProteinTech Group, Inc), claudin (CLDN)1 (1:1,500, cat. no. 13050‐1‐AP, ProteinTech Group, Inc), intercel‐ lular adhesion molecule (ICAM)1 (1:2,000, cat. no. 60299‐1‐Ig, ProteinTech Group, Inc), syndecan (SDC)4 (1:1,000, cat. no. 11820‐1‐AP, ProteinTech Group, Inc) or β‐actin (1:1,000, cat. no. TA‐09; OriGene Technologies, Inc.).

Techniques: Over Expression, Western Blot, Negative Control