Journal: Oncotarget

Article Title: Down-regulation of CITED2 attenuates breast tumor growth, vessel formation and TGF-β-induced expression of VEGFA

doi: 10.18632/oncotarget.14048

Figure Lengend Snippet: ( A ) Average tumor volume measured over time following injection of scramble or shCITED2-expressing MDA-MB-231 (left) and MDA-MB-468 (right) cells into the mammary fat pad of athymic nude mice. ( B ) Representative H&E-stained sections of tumor from each experimental group. The pale pink areas highlighted by yellow dotted lines denote deceased tissues. The adjacent histogram represents the average percentage of deceased tissue displayed by the tumors in each experimental group as measured on H&E-stained sections. ( C ) Representative images of immunohistochemical analysis of Ki67 protein expression from each experimental group. Ki67 was identified using DAB (brown) and visualized by light microscopy. The adjacent histogram represents the average percentage of Ki67 positive cells displayed in each experimental group. (** P < 0.01, *** P < 0.001). Scale bar: 4 mm.

Article Snippet: Endogenous biotin was then blocked using an Avidin and Biotin blocking kit (DAKO) for 10 minutes and incubated at 37°C for one hour with rabbit anti-human ki67 antibody (1:20 dilution, NB500-0170, Novus Biologicals).

Techniques: Injection, Expressing, Staining, Immunohistochemical staining, Light Microscopy

Journal: Biology Open

Article Title: Loss of p19Arf promotes fibroblast survival during leucine deprivation

doi: 10.1242/bio.058728

Figure Lengend Snippet: Loss of p19Arf enhances fibroblast proliferation and tumor cell proliferation during leucine deprivation (LD). (A) Quantification of trypan blue negative WT or p19Arf −/− lung fibroblasts on the days indicated after normal media (left) or LD media (right). Fold change is relative to day 0 cell counts. (B) Representative images of EdU uptake (16-h pulse) by WT or p19Arf −/− lung fibroblasts after LD for the indicated days. Scale bars: 50 µm. (C) Representative images of EdU uptake by WT or p19Arf −/− lung fibroblasts during LD plus mitomycin C (Mito C). Fold change is relative to day 0. Scale bars: 50 μm. (D) Quantification of Annexin V + WT or p19Arf −/− fibroblasts in the presence of complete or LD media. N =3. (E) Representative images of dissociated cells from tumoroids stained for Ki67 Quantification is shown on the right. Scale bars: 50 µm. N =5; *, P <0.05; ***, P <0.001; ns, not significant.

Article Snippet: Tumoroids were collected and dissociated to acquire single-cell suspension, concentrated via cytospin, and stained for Ki67 (1:100, Novus Biologicals, NB110-89717) Alexa Fluor 488-doneky-anti-sheep IgG (1:500, Novus Biologicals, NBP1-75446 coverslips were mounted face down onto microscope slides using Vectashield anti-fade mounting medium (Vector Laboratories).

Techniques: Staining

Journal: Cancer Research Communications

Article Title: Novel Syngeneic Cell Lines for Studying High-Risk BRAF V600E -Driven Colorectal Cancer In Vivo

doi: 10.1158/2767-9764.CRC-25-0599

Figure Lengend Snippet: All NaJa cell lines successfully engraft into syngeneic immunocompetent C57BL/6N mice and recapitulate their individual 2D morphologies in vivo . A, Schematic overview of the PVI of NaJa cells ( n = 2 NaJa-D, n = 10 NaJa-F, and n = 3 NaJa-G). B, Left, livers showing metastatic nodules 4 weeks after injection (black arrows) and invasive growth in the pancreatic tissue (blue circles). Scale bar, 1 cm. Hematoxylin and eosin: Histology showing that all NaJa cell lines establish tumors (pale blue) in the liver parenchyma (pink) with a differentiation state matching their epithelial or mesenchymal in vitro morphology. Right, IHC and immunofluorescence (IF) staining of proliferation marker Ki-67 (left) and intestinal differentiation markers CDX2 (middle) and E-cadherin (right). L, liver; T, tumor. Scale bar (overview), 500 μm, scale bar (zoom, IHC, and IF), 50 μm. C, Quantification of Ki-67 and CDX2 IHC staining in NaJa cell–induced liver metastases. Each dot represents an individual tumor; different shapes indicate individual tissue sections, and colors denote individual mice. Data are shown as the median ±95% confidence interval (CI).Statistical significance was assessed using the Kruskal–Wallis test followed by Dunn multiple comparison test. **, P < 0.01; ****, P < 0.0001. D, IHC staining of ERK and its downstream target DUSP6. Red arrows indicate examples of nuclear ERK staining. L, liver; T, tumor. Scale bar, 50 μm. [ A, Created by J. Traichel in BioRender. Brummer, T. (2025) https://BioRender.com/8ogg64c .]

Article Snippet: The formalin-fixed, paraffin-embedded (FFPE) sections underwent standard processing and staining procedures using the following primary antibodies directed against E-cadherin (1:50, #610181, BD Laboratories; RRID: AB_397580) and Ki-67 (1:400, #9129, Cell Signaling Technology; RRID: AB_2687446).

Techniques: In Vivo, Injection, In Vitro, Immunofluorescence, Staining, Marker, Immunohistochemistry, Comparison