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Image Search Results
Journal: Journal of Advanced Research
Article Title: The effects of primary cilia-mediated mechanical stimulation on nestin + -BMSCs during bone-tendon healing
doi: 10.1016/j.jare.2024.09.012
Figure Lengend Snippet: (A to C) The transverse and longitudinal migration ability of nestin + -BMSCs were measured by scratch assay and transwell assay, respectively. (D) The proliferative activity of nestin + -BMSCs was detected by CCK8. (E & F) Alcian blue staining, alizarin red staining and gene expression levels detected by qRT-PCR were used to analyze the chondrogenic and osteogenic differentiation potential of nestin + -BMSCs. (G) The expression of primary cilia in nestin + -BMSCs was detected by ace-tubulin fluorescence staining. Bar indicated 20 μm. (H) Expression of actin in nestin + -BMSCs stained with TRITC labeled Phalloidin Rhodamine. Bar indicated 50 μm. (I) The expression of acive-YAP in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (J) The expression of TAZ in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (K & L) Expression of key components of Hippo signaling pathway in nestin + -BMSCs. *, p < 0.05; #, p < 0.01.
Article Snippet: The expression of IFT88 and
Techniques: Migration, Wound Healing Assay, Transwell Assay, Activity Assay, Staining, Gene Expression, Quantitative RT-PCR, Expressing, Fluorescence, Labeling, Immunofluorescence
Journal: Journal of Advanced Research
Article Title: The effects of primary cilia-mediated mechanical stimulation on nestin + -BMSCs during bone-tendon healing
doi: 10.1016/j.jare.2024.09.012
Figure Lengend Snippet: (A & B) Inhibitory effect of actin on active-YAP and TAZ expression in nestin + -BMSCs. Bar indicated 50 μm. (C & D) Inhibitory effect of actin on expression of key components of Hippo signaling pathway in nestin + -BMSCs. (E) The effect of inhibiting Hippo signaling pathway on nestin + -BMSCs’ proliferation activity was detected by CCK8. (F to H) The effects of Hippo signaling inhibition on nestin + -BMSCs’ migration were detected by scratch assay and transwell assay. (I & J) The effects of Hippo signaling inhibition on nestin + -BMSCs’ differentiation potential were detected by alcian blue staining, alizarin red staining and gene expression levels. *, p < 0.05; #, p < 0.01.
Article Snippet: The expression of IFT88 and
Techniques: Expressing, Activity Assay, Inhibition, Migration, Wound Healing Assay, Transwell Assay, Staining, Gene Expression
Journal: eLife
Article Title: Neural control of growth and size in the axolotl limb regenerate
doi: 10.7554/eLife.68584
Figure Lengend Snippet:
Article Snippet: Software, algorithm ,
Techniques: In Situ, Software, Plasmid Preparation, Modification