Journal: bioRxiv

Article Title: Effect of hydrogen inhalation on IL-40 and SIgA in a Rat Model of Pulmonary Mucosal Immunity

doi: 10.1101/2020.06.29.177345

Figure Lengend Snippet: Effect of hydrogen on the expression of SIGA and PIgR in the lung obtained from the COPD rat model, determined by immunohistochemical staining or Western blotting. A Immunohistochemical staining of the lung sections for SIGA, and Western blotting of the lung sections for PIgR. B Quantitative analysis of the expression of SIGA and PIgR in the COPD rat model. (n=10 for each group. ## P <0.01 compared with COPD group; ** P <0.01). Each experiment was repeated three times and similar results were obtained.

Article Snippet: Nitrogen (15% O 2 , 85% N 2 ) was purchased from Shijiazhuang Central Plains Specialty Gases Ltd., and a 2% H 2 gas mixture (21% O 2 , 2% H 2 , 77% N 2 ) was purchased from Guangzhou Puyuan Gas Company Ltd. Anti-IgA secretory component (Mouse, ab212330, 1:500 in IHC, Abcam), anti-IL-4 (Mouse, sc53084, 1:100 in IHC, 1:500 in WB; SanTaCruz), anti-PIGR (Rabbit, bs-6061R, 1:500 in WB; Bioss), anti-IL-5 (Rabbit, GTX55678, 1:100 in IHC, 1:500 in WB; GeneTex), anti-TGF-β1 (Rabbit, ARG56429, 1:500 in WB; Arigo), anti-C17orf99 (Rabbit, PA5-71295, 1:500 in WB; ThermoFisher) primary antibodies, and anti-rabbit and anti-mouse secondary antibodies (Abbkine, Reddlands, CA, USA) were used to determine protein expression.

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

Journal: Skin Research and Technology

Article Title: Rutin alleviates psoriasis‐related inflammation in keratinocytes by regulating the JAK2/STAT3 signaling

doi: 10.1111/srt.70011

Figure Lengend Snippet: M5‐induced abnormal differentiation of HaCaT cells was ameliorated following Rutin treatment. (A–C) Western blotting assessed the protein levels of Keratin1 and Keratin5 in M5‐stimulated HaCaT cells and Rutin‐treated M5‐stimulated HaCaT cells. *** p < 0.001 vs. control; ## p < 0.01 and ### p < 0.001 vs. M5. Differential expression testing was performed with ANOVA.

Article Snippet: Subsequently, the membrane underwent blocking, followed by detection with specific primary antibodies, including Keratin5 (Cat#GTX11321, 1:1000, GeneTex, Irvine, CA, USA), Keratin1 (Cat#M01639‐1, 1:5000, BosterBio, Wuhan, China), p‐Janus kinase 2 (JAK2) (Cat#GTX132784, 1:1000, GeneTex), p‐signal transducer and activator of transcription 3 (STAT3) (Cat#bs‐55208R, 1:1000, Bioss, Beijing, China), and β‐actin (Cat#ab8226, 1:10 000, Abcam, Cambridge, Massachusetts, USA).

Techniques: Western Blot, Control, Quantitative Proteomics