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Medicago
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Millar Inc
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Babco Inc
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Millar Inc
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Millar Inc
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Johns Hopkins HealthCare
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ScienCell
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Kongsberg Maritime Contros GmbH
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LifeNet Health Inc
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ZenBio
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CooperSurgical
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Image Search Results
Journal: Cancer Research
Article Title: Efficacious Immune Therapy in Chronic Myelogenous Leukemia (CML) Recognizes Antigens That Are Expressed on CML Progenitor Cells
doi: 10.1158/0008-5472.can-09-2303
Figure Lengend Snippet: Figure 4. VPS4B, DUSP12, RAB38, RPS6KC1, and TBCE are overexpressed in CML progenitor cells. Relative gene expression was detected by gene expression microarrays among PBMC (n = 5), bone marrow (n = 5), normal monocytes (Monos; n = 3), granulocytes (Neuts; n = 3), AML (n = 9), a CML cell line (K562; n = 4), CML CD34+ cells (n = 12), and normal CD34+ cells (n = 8). Significance was determined by Student's t test.
Article Snippet: Protein bands were visualized using antigen-specific antibodies against DUSP12 (1:4,000; Abcam), RAB38 (clone 11B-7, 1:200; Santa Cruz Biotechnology), and
Techniques: Gene Expression
Journal: Cancer Research
Article Title: Efficacious Immune Therapy in Chronic Myelogenous Leukemia (CML) Recognizes Antigens That Are Expressed on CML Progenitor Cells
doi: 10.1158/0008-5472.can-09-2303
Figure Lengend Snippet: Figure 5. DUSP12, RAB38, and TBCE have enriched expression in CML CD34+ cells and elicit antibody responses in patients developing treatment responses against CML. A, post-DLI plasma of patient C has greater reactivity than either pre-HSCT or pre-DLI against DUSP12, RAB38, and TBCE, shown by immunoprecipitation and Western blotting. Antibody reactivity was temporally correlated with response to therapy (PCR, molecular detection of BCR-ABL transcript; Cyto, detection of the Philadelphia chromosome). The EBV-derived protein EBNA1 was used as a positive control antigen. B, increased protein expression of DUSP12, RAB38, and TBCE was seen in CML CD34+ cells and other malignant myeloid cells compared with normal CD34−cells and CML CD34−cells. Increased protein expression of DUSP12 was also seen in CML CD34+ cells compared with normal CD34+ cells, although protein expression of RAB38 and TBCE was equivalent in both normal and malignant CD34+ cells. C, antibody reactivity against recombinant GST-tagged DUSP12, RAB38, and TBCE is observed in other patients with effective CML immunity but not in normal donors and only rarely in allotransplanted patients without CML, as shown by immunoprecipitation of individual recombinant proteins with patient plasma followed by Western blotting. Dark gray shading, strong plasma binding of antigen; light gray shading, moderate plasma binding of antigen; no shading, plasma binding at or below background levels. Data are normalized to plasma GST binding.
Article Snippet: Protein bands were visualized using antigen-specific antibodies against DUSP12 (1:4,000; Abcam), RAB38 (clone 11B-7, 1:200; Santa Cruz Biotechnology), and
Techniques: Expressing, Clinical Proteomics, Immunoprecipitation, Western Blot, Derivative Assay, Positive Control, Recombinant, Binding Assay