Journal: Biophysical Journal

Article Title: Kinetics of K+ Occlusion by the Phosphoenzyme of the Na+,K+-ATPase

doi: 10.1016/j.bpj.2010.11.038

Figure Lengend Snippet: Sequential mixing stopped-flow fluorescence transients of Na + ,K + -ATPase from pig kidney and shark rectal gland noncovalently labeled with RH421. Na + ,K + -ATPase (60 μ g/mL) was premixed with a small volume of 0.5 M Na 2 ATP to a final concentration of 4 mM ATP. A few seconds later, the enzyme was mixed with an equal volume of a KCl solution (0.6 mM after mixing). The final Na + ,K + -ATPase concentration was 30 μ g/mL, and the RH421 concentration was 400 nM. Both the enzyme suspensions and the KCl solutions were in a buffer containing 30 mM imidazole, 40 mM NaCl, 5 mM MgCl 2 , and 1 mM EDTA (pH 7.4, 24°C). The total ionic strength was maintained at a constant value by the addition of choline chloride to the enzyme suspension and the KCl solutions ([choline chloride] + [KCl] = 90 mM). The fluorescence of membrane-bound RH421 was measured at an excitation wavelength of 577 nm at emission wavelengths of ≥665 nm (RG665 glass cutoff filter). The calculated observed rate constants were 67 (±2) s −1 for the pig enzyme and 120 (±11) s −1 (82% of the total amplitude) and 16 (±3) s −1 (18%) for the shark enzyme.

Article Snippet: The origins of the various reagents used were: imidazole (≥99%; Sigma, Castle Hill, Australia), NaCl (suprapure; Merck, Kilsyth, Australia), KCl (analytical grade; Merck), choline chloride (99%; Sigma), MgCl2 ·6H2 O (analytical grade; Merck), EDTA (99%; Sigma), ATP disodium·3H2 O (special quality; Roche, Castle Hill, Australia), NaOH (analytical grade; Merck), and HCl (0.1 N Titrisol solution; Merck).

Techniques: Flow Cytometry, Fluorescence, Labeling, Concentration Assay

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Learning-based recovery from perceptual impairment in salt discrimination after permanently altered peripheral gustatory input

doi: 10.1152/ajpregu.00843.2009

Figure Lengend Snippet: Overall performance for experiment 2 amiloride sessions. Mean overall percentage correct (+SE) in NaCl vs. KCl (dissolved in 100 μM amiloride) discrimination by group for presurgical ( left ) and postsurgical ( right ) amiloride sessions. *Significantly

Article Snippet: The rats in experiments 1 and 2 were trained to associate one reinforcement spout with NaCl and the other with KCl (both reagent grade chemicals; Fisher Scientific, Orlando, FL).

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Learning-based recovery from perceptual impairment in salt discrimination after permanently altered peripheral gustatory input

doi: 10.1152/ajpregu.00843.2009

Figure Lengend Snippet: Overall performance for experiment 1 control sessions. Mean overall percentage correct (+SE or ±SE) in NaCl vs. KCl discrimination by group for presurgical control sessions ( left ), initial postsurgical testing ( weeks 1–4 ; middle ), and

Article Snippet: The rats in experiments 1 and 2 were trained to associate one reinforcement spout with NaCl and the other with KCl (both reagent grade chemicals; Fisher Scientific, Orlando, FL).

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Learning-based recovery from perceptual impairment in salt discrimination after permanently altered peripheral gustatory input

doi: 10.1152/ajpregu.00843.2009

Figure Lengend Snippet: Overall performance for experiment 2 control sessions. Mean overall percentage correct (+SE or ±SE) in NaCl vs. KCl discrimination by group for presurgical control sessions ( left ), initial postsurgical testing ( weeks 1–4 ; middle ), and

Article Snippet: The rats in experiments 1 and 2 were trained to associate one reinforcement spout with NaCl and the other with KCl (both reagent grade chemicals; Fisher Scientific, Orlando, FL).

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Learning-based recovery from perceptual impairment in salt discrimination after permanently altered peripheral gustatory input

doi: 10.1152/ajpregu.00843.2009

Figure Lengend Snippet: Overall performance for experiment 1 amiloride sessions. Mean overall percentage correct (+SE) in NaCl vs. KCl (dissolved in 100 μM amiloride) discrimination by group for presurgical ( left ) and postsurgical ( right ) amiloride sessions. *Significantly

Article Snippet: The rats in experiments 1 and 2 were trained to associate one reinforcement spout with NaCl and the other with KCl (both reagent grade chemicals; Fisher Scientific, Orlando, FL).

Techniques:

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Taurine relaxes human radial artery through potassium channel opening action

doi: 10.4196/kjpp.2017.21.6.617

Figure Lengend Snippet: Vasorelaxations to cumulative concentrations of taurine (20, 40, 80 mM) in human radial artery rings precontracted with 45 mM KCl in the presence of tetraethylammonium (TEA, 1 mM), 4-aminopyridine (4-AP, 1 mM), barium chloride (BaCl 2 , 30 µM) and glibenclamide (GLI, 10 µM). Each value represents mean±S.E.M. and it is calculated as the percentage of the first KCl (45 mM)-induced contraction in the same ring (n=7~11). Vertical bars represent S.E.M. * p

Article Snippet: KCl and TEA were obtained from Merck (Darmstadt, Hessen, Germany).

Techniques:

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Taurine relaxes human radial artery through potassium channel opening action

doi: 10.4196/kjpp.2017.21.6.617

Figure Lengend Snippet: Original tracings showing vasorelaxations to taurine (20, 40, 80 mM) in KCl (45 mM)-precontracted human radial artery rings in the absence (control) and presence of tetraethylammonium (TEA, 1 mM).

Article Snippet: KCl and TEA were obtained from Merck (Darmstadt, Hessen, Germany).

Techniques:

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Catalytic Activity of Alkali Metal Cations for the Chemical Oxygen Reduction Reaction in a Biphasic Liquid System Probed by Scanning Electrochemical Microscopy

doi: 10.1002/chem.202001967

Figure Lengend Snippet: (a) Chronoamperograms for the H 2 O 2 oxidation at the ME after ion‐transfer and H 2 O 2 generation at the liquid|liquid interface by using the cell in Figure 2 ; the aqueous solution was (I) LiCl, (II) NaCl, (III) KCl, and (IV) HCl at d ME–MP values of (1) 2, (2) 10, (3) 20, (4) 30, (5) 40, (6) 50, and (7) 100 μm. (b) Steady‐state oxidation current from the chronoamperograms in (a) as a function of d ME–MP for different aqueous electrolytes; Pt ME with r T =12.5 μm, E T =0.8 V (vs. Ag|AgCl|Cl − ), Δ o w φ =−0.65 V, and r MP =10 μm.

Article Snippet: ChemicalsNaCl ( > 99.99 %, Carl Roth), LiCl ( > 9999 %, VWN chemicals), KCl ( > 99.99 %, Carl Roth), HCl (35 %, VWN chemicals), DMFc (Sigma–Aldrich), LiTB (Sigma–Aldrich), bis(triphenylphosphoranylidene) ammonium chloride (BACl, Sigma–Aldrich), and DCE (Sigma–Aldrich) were used as received.

Techniques:

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Catalytic Activity of Alkali Metal Cations for the Chemical Oxygen Reduction Reaction in a Biphasic Liquid System Probed by Scanning Electrochemical Microscopy

doi: 10.1002/chem.202001967

Figure Lengend Snippet: (a) Approach curves (1) without and (2) with Δ o w φ =−0.65 V applied at the liquid|liquid interface using the cell in Figure 2 with LiCl as an aqueous electrolyte solution. (b) The same as (a) but with aqueous electrolyte solutions of (1) HCl, (2) KCl, (3) NaCl, and (4) LiCl; Pt ME with r tip =12.5 μm as WE2, E T =0.8 V (vs. Ag|AgCl|Cl − ), v T =0.8 μm s −1 , Δ o w φ =−0.65 V, r MP ≈50 μm for (a) and 10 μm for (b).

Article Snippet: ChemicalsNaCl ( > 99.99 %, Carl Roth), LiCl ( > 9999 %, VWN chemicals), KCl ( > 99.99 %, Carl Roth), HCl (35 %, VWN chemicals), DMFc (Sigma–Aldrich), LiTB (Sigma–Aldrich), bis(triphenylphosphoranylidene) ammonium chloride (BACl, Sigma–Aldrich), and DCE (Sigma–Aldrich) were used as received.

Techniques:

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Catalytic Activity of Alkali Metal Cations for the Chemical Oxygen Reduction Reaction in a Biphasic Liquid System Probed by Scanning Electrochemical Microscopy

doi: 10.1002/chem.202001967

Figure Lengend Snippet: Plot of H 2 O 2 oxidation at the ME in the cell in Figure 2 for aqueous solutions of LiCl, NaCl, KCl, and HCl as a function of standard hydration enthalpy Δ hyd H ° of protons and alkali metal ions from ref. 25 . The values of i T are taken from Figure 5 b at d ME–MP =2 μm. The solid line is a fit for LiCl, NaCl, and KCl i T [pA]=−0.21 Δ hyd H ° [kJ mol −1 ]−45.70 ( R 2 =0.995). The dashed line is an extrapolation of the solid line for RbCl and CsCl solutions and the literature values of Δ hyd H °(Rb + ) and Δ hyd H °(Cs + ).

Article Snippet: ChemicalsNaCl ( > 99.99 %, Carl Roth), LiCl ( > 9999 %, VWN chemicals), KCl ( > 99.99 %, Carl Roth), HCl (35 %, VWN chemicals), DMFc (Sigma–Aldrich), LiTB (Sigma–Aldrich), bis(triphenylphosphoranylidene) ammonium chloride (BACl, Sigma–Aldrich), and DCE (Sigma–Aldrich) were used as received.

Techniques:

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Catalytic Activity of Alkali Metal Cations for the Chemical Oxygen Reduction Reaction in a Biphasic Liquid System Probed by Scanning Electrochemical Microscopy

doi: 10.1002/chem.202001967

Figure Lengend Snippet: (a) ITCV with 0.1 m of (1) KCl, (2) HCl, (3) LiCl, and (4) NaCl as aqueous electrolyte solution in the absence of DMFc in the organic phase. (b) ITCV with the cell shown in Figure 2 and 0.1 m of (1) KCl, (2) HCl, (3) LiCl, and (4) NaCl as aqueous electrolyte solution in the presence of 5 m m DMFc in the organic phase; r MP ≈10 μm and v =20 mV s −1 .

Article Snippet: ChemicalsNaCl ( > 99.99 %, Carl Roth), LiCl ( > 9999 %, VWN chemicals), KCl ( > 99.99 %, Carl Roth), HCl (35 %, VWN chemicals), DMFc (Sigma–Aldrich), LiTB (Sigma–Aldrich), bis(triphenylphosphoranylidene) ammonium chloride (BACl, Sigma–Aldrich), and DCE (Sigma–Aldrich) were used as received.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Osmotic and Salt Stresses Modulate Spontaneous and Glutamate-Induced Action Potentials and Distinguish between Growth and Circumnutation in Helianthus annuus Seedlings

doi: 10.3389/fpls.2017.01766

Figure Lengend Snippet: Spontaneous action potentials and glutamate induced series of action potentials in Helianthus annuus seedlings under osmotic and salt stresses. (A) Helianthus annuus seedlings, electrode arrangement (1, 2, ref—reference electrode) and site of glutamate injection (i). (B) Example of recordings of spontaneous action potentials during 1 day (also shown in Supplementary Video S2 ). In the parentheses, the time of SAP appearing. (C) Example of recordings of acropetally and basipetally propagating spontaneous action potentials. (D) Example of recordings of action potential series after glutamate (Glu) injection into the hypocotyl base of seedlings growing under 80 mM KCl (upper) as well as 160 mM NaCl nutrient solutions (lower). Data details are presented in Tables 1 , 2 .

Article Snippet: Injection Twenty microliters of a 50 mM Glu solution (l -glutamic acid, ICN Biomedicals, Germany, pH adjusted to 7 by Tris/Mes buffer), distilled water, 250 mM KCl (500 mOsm) (POCH, Poland), 250 mM NaCl (500 mOsm) (POCH, Poland), and 500 mM D-sorbitol (500 mOsm) (POCH, Poland) were injected with a syringe into the seedlings at the base of the hypocotyls (Glu), 1 cm above the root collar, or at the base of the stem in the 3-week old plants.

Techniques: Injection

Journal: Frontiers in Plant Science

Article Title: Osmotic and Salt Stresses Modulate Spontaneous and Glutamate-Induced Action Potentials and Distinguish between Growth and Circumnutation in Helianthus annuus Seedlings

doi: 10.3389/fpls.2017.01766

Figure Lengend Snippet: Osmotically and potassium chloride-induced series of action potentials in 3-week old Helianthus annuus . (A) Helianthus annuus plants, electrode arrangement (1, 2, 3, 4, ref—reference electrode) and site of solution injection (i). (B) Example of recordings of action potential series after D-sorbitol 500 mOsm (500 mM) injection into the stem base. (C) Example of recordings of action potential series after KCl 500 mOsm (250 mM) injection into the stem base. Data details are presented in Table 3 .

Article Snippet: Injection Twenty microliters of a 50 mM Glu solution (l -glutamic acid, ICN Biomedicals, Germany, pH adjusted to 7 by Tris/Mes buffer), distilled water, 250 mM KCl (500 mOsm) (POCH, Poland), 250 mM NaCl (500 mOsm) (POCH, Poland), and 500 mM D-sorbitol (500 mOsm) (POCH, Poland) were injected with a syringe into the seedlings at the base of the hypocotyls (Glu), 1 cm above the root collar, or at the base of the stem in the 3-week old plants.

Techniques: Injection

Journal: The Journal of Biological Chemistry

Article Title: The Impact of N-terminal Acetylation of α-Synuclein on Phospholipid Membrane Binding and Fibril Structure *

doi: 10.1074/jbc.M116.726612

Figure Lengend Snippet: Membrane binding characteristics of WT-αS, NTAc-αS, and Endo-αS. All data obtained with WT-αS are depicted with black diamonds / lines , NTAc-αS with red squares / lines, and Endo-αS with blue circles / lines. Open symbols in panels A, C , and E represent data obtained in the presence of LUVs and closed symbols represent data obtained in presence of SUVs. Binding curves of αS to 100% POPS liposomes ( panel A ), 25:75 POPC:POPS ( panel B ), 50:50 POPC:POPS ( panel C ), and 75:25 POPC:POPS ( panel D ) showing no differences in membrane binding of acetylated-αS compared with WT-αS. Binding curves of αS to POPC liposomes show hardly any binding of αS to membranes of this composition but acetylated-αS has a slightly higher affinity for 100% POPC SUVs than WT-αS ( panel E ). Average MRE values were obtained from the plateau phase of the binding curve obtained from CD spectroscopy measurements showing insignificant differences for either WT-αS or acetylated-αS indicating a similar size of helical domain on lipid membranes ( panel F ). All measurements were performed at room temperature in the presence of 10 m m Tris, 100 m m KCl buffered at pH 7.4. The error bars in all binding curves represent standard deviations from 3 independent measurements. The binding curves for LUVs ( open symbols ) shown in panels C and E could not be fitted using the solution to a simple quadratic equation ( 23 ) and the depicted lines are only a guide to the eye.

Article Snippet: Tris salt and potassium chloride (KCl) were purchased from Merck (Germany).

Techniques: Binding Assay, Spectroscopy

Journal: The Journal of Biological Chemistry

Article Title: The Impact of N-terminal Acetylation of α-Synuclein on Phospholipid Membrane Binding and Fibril Structure *

doi: 10.1074/jbc.M116.726612

Figure Lengend Snippet: Membrane binding characteristics of WT-αS, NTAc-αS, and Endo-αS to SUVs containing cholesterol. A, binding curves comparing the affinity of αS to 1:1 Chol:POPS SUVs ( open symbols ) and 1:1 POPC:POPS SUVs ( closed symbols ). B, binding curves of αS to 1:1 POPC:Chol SUVs ( open symbols ) and POPC SUVs ( closed symbols ). The binding curves for SUVs ( open symbols ) shown in panel B could not be fitted using the solution to a simple quadratic equation ( 26 ) and the depicted lines are only a guide to the eye. All measurements were performed at room temperature in the presence of 10 m m Tris, 100 m m KCl buffered at pH 7.4. The error bars in all binding curves represent standard deviations from 3 independent measurements.

Article Snippet: Tris salt and potassium chloride (KCl) were purchased from Merck (Germany).

Techniques: Binding Assay

Journal: Nucleic Acids Research

Article Title: Controlling the stoichiometry and strand polarity of a tetramolecular G-quadruplex structure by using a DNA origami frame

doi: 10.1093/nar/gkt592

Figure Lengend Snippet: The snapshots of the real-time HS-AFM imaging of the conformational changes. ( a ) Salt-induced formation of a G-quadruplex. The origami was prepared and immobilized on mica surface in a KCl-free buffer, while the imaging was carried out in a buffer that contained 100 mM KCl. ( b ) The deformation of a G-quadruplex structure under KCl-free environment. The origami was prepared and immobilized on mica surface in a buffer containing 100 mM KCl, whereas the observation buffer contained no KCl. The long duplex system (67-mer top and 77-mer bottom duplexes) with six G-repeats was used in these studies. The numbers at the top left corner represent the imaging time in second. Image size: 125 × 125 nm; scan speed: 0.2 frame/s. [MgCl 2 ] = 10 mM; [Tris–HCl] = 20 mM, pH 7.6. For real-time movies, see Supplementary section .

Article Snippet: Chemicals and reagents Tris–HCl, EDTA, MgCl2 and KCl were purchased from Nacalai Tesque, Inc. (Kyoto, Japan).

Techniques: Imaging

Journal: Nucleic Acids Research

Article Title: Controlling the stoichiometry and strand polarity of a tetramolecular G-quadruplex structure by using a DNA origami frame

doi: 10.1093/nar/gkt592

Figure Lengend Snippet: Control experiment with six G–T (top duplex) and T–T (bottom duplex) mismatch-containing duplexes in the presence of 100 mM KCl. Long duplex system was used. The mismatch regions are shown above the images, and other regions in the sequence are same as in Figure 1 a. The absence of X-shape indicates the absence of the quadruplex structure. Zoom-in image size: 125 × 125 nm. [KCl] = 100 mM (both in origami solution and observation buffer); [MgCl 2 ] = 10 mM; [Tris–HCl] = 20 mM, pH 7.6.

Article Snippet: Chemicals and reagents Tris–HCl, EDTA, MgCl2 and KCl were purchased from Nacalai Tesque, Inc. (Kyoto, Japan).

Techniques: Sequencing

Journal: Nucleic Acids Research

Article Title: Controlling the stoichiometry and strand polarity of a tetramolecular G-quadruplex structure by using a DNA origami frame

doi: 10.1093/nar/gkt592

Figure Lengend Snippet: The Zoom-out AFM images of the DNA origami frame with incorporated duplexes recorded in the absence ( a, b ) and presence ( c ) of KCl for the G-repeat number of six. The parallel-shape of the incorporated strands can be clearly seen in the absence of KCl, indicating that no G-quadruplex is formed in this case. The X-shape in the presence of KCl evidences the formation of the quadruplex structure. The representative zoom-in images recorded in the absence of KCl for the sequences that contained six ( d ), four ( e ) and three ( f ) G-repeats. The same sequences in the presence of KCl are given, respectively, in ( g–i ). ( j ) The height profile estimated from the image given above the graph indicates that the origami frame and the incorporated duplexes are nearly same in height. ( k–l ) Height profiles estimated (vertical: k, and horizontal: l) indicate that the X-shape is slightly taller than the origami frame. This could be due to the formation of the four-stranded G-quadruplex which is taller than the duplexes in origami. ( m ) The height profiles of the duplexes and G-quadruplex regions. Yellow lines in the images indicate the locations where the height analyses were performed. The numbers in the graphs represent the estimated heights in nm of the peaks from the respective base line. All the images given in this figure were recorded for 67-mer top and 77-mer bottom duplexes. Image size: 125 × 125 nm (d–m). [KCl] = 0 or 100 mM; [MgCl 2 ] = 10 mM; [Tris-HCl] = 20 mM, pH 7.6.

Article Snippet: Chemicals and reagents Tris–HCl, EDTA, MgCl2 and KCl were purchased from Nacalai Tesque, Inc. (Kyoto, Japan).

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Region-dependent dynamics of cAMP response element-binding protein phosphorylation in the basal ganglia

doi:

Figure Lengend Snippet: Contrasting effects of treatments inducing calcium flux on CREB phosphorylation in the caudoputamen ( A – C ) and in the olfactory tubercle ( A ′– C ′). Both KCl ( A ) and NMDA ( B ) induce high levels of PCREB expression through the caudoputamen. In sharp contrast, KCl induces little PCREB expression ( A ′) and NMDA induces only weak PCREB expression ( B ′) in the olfactory tubercle. Pretreatment with EGTA before BAY K 8644 stimulation decreases the PCREB immunostaining to background levels in the caudoputamen ( C ), but significantly enhances PCREB immunoreactivity in the islands of the olfactory tubercle (curved arrow in C ′). All drug treatments were for 30 min. The arrowheads in A ′– C ′ point to ventral edges of the tissue; × indicates a region of tissue damage. NAc, nucleus accumbens; AC, anterior commissure; fb, fiber bundles. [Bar in C (for A – C ) = 100 μm; bar in C ′ (for A ′– C ′) = 0.5 mm.]

Article Snippet: For the initial time-course study, striatal slices were incubated for 7, 15, or 30 min, or 1, 2, 4, 8, or 24 hr, with the following reagents: the D1-selective agonist, SKF-81297 HCl (100 nM, kindly provided by J. Weinstock of SmithKline Beecham); the D2-selective agonist, R(−)-quinpirole HCl [10 μM; Research Biochemicals (RBI), Natick, MA]; the L-type voltage-sensitive Ca2+ channel agonist, S(−)-BAY K 8644 (1 μM; RBI); the adenylate cyclase activator, forskolin (10 μM; RBI); KCl (50 mM; Mallinckrodt); or the N -methyl- d -aspartate receptor agonist, NMDA (10 μM, 100 μM; RBI).

Techniques: Expressing, Immunostaining

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Synaptic mechanisms of temporal diversity in the lateral geniculate nucleus of the thalamus

doi: 10.1523/JNEUROSCI.4046-12.2013

Figure Lengend Snippet: Early synaptic inhibition is mediated by chloride. Recording from a X L cell with 3M KCl pipette solution. A) Three minutes after impalement, the leading inhibition to a same-sign contrast center stimulus was evident as shown by PSTH (top), the spike raster

Article Snippet: We further investigated the ionic basis of the early inhibition at a cellular level by recording from 4 XL cells with KCl (3M) in the pipette rather than the control KAc solution.

Techniques: Inhibition, Transferring

Journal: Haematologica

Article Title: BCR-ABL1 mediated miR-150 downregulation through MYC contributed to myeloid differentiation block and drug resistance in chronic myeloid leukemia

doi: 10.3324/haematol.2018.193086

Figure Lengend Snippet: miR-150 regulation by MYC in leukemic blasts. MYC occupancy at putative regulatory loci of the MIR150 gene in (A) K562, (B) KCL-22 and (C) HL-60 cells with or without imatinib treatment. Columns represent the fold change (FC) in the % of DNA input obtained in the control (gray) or imatinib-treated (dark) cells compared with the non-specific IgG precipitation and equalized to 1 (dashed line). The promoter region of the miR-15a/16-1 cluster was used as the positive control of MYC DNA binding. Unpaired two-tailed Student t -test was used to determine P -values. * P

Article Snippet: The BCR-ABL1-positive CML cell lines K562, MEG-01 and KCL-22 and the BCR-ABL1-negative AML cell lines HL-60 and KG-1 were obtained from a publicly accessible biological resource center (Leibniz Institute - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH/DSMZ, Braunschweig, Germany).

Techniques: Positive Control, Binding Assay, Two Tailed Test

Journal: Haematologica

Article Title: BCR-ABL1 mediated miR-150 downregulation through MYC contributed to myeloid differentiation block and drug resistance in chronic myeloid leukemia

doi: 10.3324/haematol.2018.193086

Figure Lengend Snippet: The effects of miR-150 overexpression and BCR-ABL1 silencing on the expression of genes in oncogenic pathways in leukemic cells. (A) MYB transcript and MYB protein expression in HL-60 cells 48 hours (h) after the miR-150 transfection. The numbers shown at the top indicate the MYB protein expression relative to that in the untreated samples (β-actin normalized). Expression of MYB transcripts (48 h) and c-MYB protein (96 h) in (B) KCL-22 and (C) K562 after miR-150 transfection and/or BCR-ABL1 activity inhibition with 1 mM imatinib. (D) MYB expression in K562 cells 48 h miR-150 transfection and/or the inhibition of BCR-ABL1 expression by siRNA. (E) miR-155 levels in KCL-22 and K562 cells 96 h after 30 nM miR-150 transfection and/or BCR-ABL1 activity inhibition with 1 μM imatinib. (F) PU.1 expression in KCL-22 and K562 cells 96 h after miR-150 transfection and/or BCR-ABL1 activity inhibition with 1 μM imatinib. (G) MYC expression in KCL-22, K562 and HL-60 cells after exposure to 1 μM imatinib. (H) Pri-miR150, pre-miR150 and miR-150 levels in K562 cells upon BCR-ABL1 inhibition. Generally, cells were transfected with 30 nM miR-150 mimic or 50 nM siRNA BCR-ABL1. The expression data represent the expression fold change (FC; −ΔΔCt ) in relation to untreated, control (Ctrl) sample normalized to 1. Unpaired two-tailed Student t -test was used to determine P -values. * P

Article Snippet: The BCR-ABL1-positive CML cell lines K562, MEG-01 and KCL-22 and the BCR-ABL1-negative AML cell lines HL-60 and KG-1 were obtained from a publicly accessible biological resource center (Leibniz Institute - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH/DSMZ, Braunschweig, Germany).

Techniques: Over Expression, Expressing, Transfection, Activity Assay, Inhibition, Two Tailed Test

Journal: Haematologica

Article Title: BCR-ABL1 mediated miR-150 downregulation through MYC contributed to myeloid differentiation block and drug resistance in chronic myeloid leukemia

doi: 10.3324/haematol.2018.193086

Figure Lengend Snippet: Comparison of the oncogenic pathway expression profiles in imatinib-sensitive (naive) K562, KCL-22 and imatinib-resistant K562R, KCL-22R chronic myeloid leukemia (CML) cell lines. BCR-ABL1, MYB, MYC, PU.1, miR-150 and miR-155 transcript levels in (A) KCL-22 and KCL-22R and in (B) K562 and K562R. Gene expression data for resistant cells represent the fold change (FC; 2 −ΔΔCt ) in relation to corresponding gene expression for naive cells normalized to 1. Unpaired two-tailed Student t -test was used to determine P -values. * P

Article Snippet: The BCR-ABL1-positive CML cell lines K562, MEG-01 and KCL-22 and the BCR-ABL1-negative AML cell lines HL-60 and KG-1 were obtained from a publicly accessible biological resource center (Leibniz Institute - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH/DSMZ, Braunschweig, Germany).

Techniques: Expressing, Two Tailed Test