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anti-tau antibodies r2295, tau5 and k9ja  (Fisher Scientific)
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Fisher Scientific anti-tau antibodies r2295, tau5 and k9ja
Mislocalized tau in periplaque dystrophic axons is critical for AD-tau-induced NP tau aggregation. (a) Representative images of endogenous tau (red) detected by antibody <t>K9JA</t> in regions with or without Aβ plaques (blue) from 5xFAD mice with no endogenous tau ((Mapt−/− 5xFAD) (8 months)) or 5xFAD mice (8 months) injected with human control brain lysate or AD-tau at 3 m.p.i. Pathological tau (green) was detected by antibody AT8. Results from one out of three mice per group are shown. (b) Similar amounts of noncompact and compact plaques were present in control-5xFAD mice, and this ratio was not significantly altered in AD-5xFAD mice. (c) Proportion of each type of Aβ plaque surrounded by K9JA-labeled endogenous tau in control-5xFAD mice. Three mice were quantified. (d,e) Representative image (d) and proportion of each type (e) of Aβ plaque (red) surrounded by induced AT8-labeled NP tau (green) in AD-5xFAD mice. DAPI (blue) stains nucleus. In d, arrows indicate noncompact plaques and arrowheads indicate compact plaques. Scale bar in d, 100 μm. Quantifications in b, c and e were performed from the ipsilateral caudal hippocampal region of 5xFAD (8 months) mice injected with AD-tau or control at 3 m.p.i.; three mice per group were quantified. (f–i) Characterization of Mapt+/− 5xFAD mice (n = 3 per group). (f) PCR of genomic DNA confirming the genotype of Mapt+/− 5xFAD mice. The primers used for genotyping are indicated on the right. (g) Western blotting showing that endogenous mouse tau is reduced in Mapt+/− 5xFAD mice as compared to Mapt+/+ 5xFAD mice. (h) Representative NAB228 immunostaining; scale bar, 400 μm. (i) Quantification of brain slices containing caudal hippocampus showing the Aβ plaque burden in 8-month-old 5xFAD mice with or without tau reduction. Two-tailed t-tests were performed; *P ≤ 0.05. (j,k) Representative AT8 immunostaining (j) and quantification (k) of NP tau in both retrosplenial cortex (ctx) and hippocampus on the ipsilateral side of AD-tau-injected 8-month-old Mapt+/+ 5xFAD and Mapt+/− 5xFAD mice at 1 m.p.i. (n = 3 per group). A dashed circle in j indicates a single NP. Scale bars in j: retrosplenial cortex, 100 μm; hippocampus, 200 μm; NP tau, 25 μm. (l) Quantification of NP-tau-positive neurites as a percentage of AT8 staining within each NP, as represented in the right panels in j. Over 100 NP tau from three mice per group were examined. A two-tailed t-test was performed; *P ≤ 0.05, ***P ≤ 0.001. Data are presented as mean ± s.e.m. in b, i, k and l.
Anti Tau Antibodies R2295, Tau5 And K9ja, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-tau antibodies r2295, tau5 and k9ja - by Bioz Stars, 2026-02
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Mislocalized tau in periplaque dystrophic axons is critical for AD-tau-induced NP tau aggregation. (a) Representative images of endogenous tau (red) detected by antibody K9JA in regions with or without Aβ plaques (blue) from 5xFAD mice with no endogenous tau ((Mapt−/− 5xFAD) (8 months)) or 5xFAD mice (8 months) injected with human control brain lysate or AD-tau at 3 m.p.i. Pathological tau (green) was detected by antibody AT8. Results from one out of three mice per group are shown. (b) Similar amounts of noncompact and compact plaques were present in control-5xFAD mice, and this ratio was not significantly altered in AD-5xFAD mice. (c) Proportion of each type of Aβ plaque surrounded by K9JA-labeled endogenous tau in control-5xFAD mice. Three mice were quantified. (d,e) Representative image (d) and proportion of each type (e) of Aβ plaque (red) surrounded by induced AT8-labeled NP tau (green) in AD-5xFAD mice. DAPI (blue) stains nucleus. In d, arrows indicate noncompact plaques and arrowheads indicate compact plaques. Scale bar in d, 100 μm. Quantifications in b, c and e were performed from the ipsilateral caudal hippocampal region of 5xFAD (8 months) mice injected with AD-tau or control at 3 m.p.i.; three mice per group were quantified. (f–i) Characterization of Mapt+/− 5xFAD mice (n = 3 per group). (f) PCR of genomic DNA confirming the genotype of Mapt+/− 5xFAD mice. The primers used for genotyping are indicated on the right. (g) Western blotting showing that endogenous mouse tau is reduced in Mapt+/− 5xFAD mice as compared to Mapt+/+ 5xFAD mice. (h) Representative NAB228 immunostaining; scale bar, 400 μm. (i) Quantification of brain slices containing caudal hippocampus showing the Aβ plaque burden in 8-month-old 5xFAD mice with or without tau reduction. Two-tailed t-tests were performed; *P ≤ 0.05. (j,k) Representative AT8 immunostaining (j) and quantification (k) of NP tau in both retrosplenial cortex (ctx) and hippocampus on the ipsilateral side of AD-tau-injected 8-month-old Mapt+/+ 5xFAD and Mapt+/− 5xFAD mice at 1 m.p.i. (n = 3 per group). A dashed circle in j indicates a single NP. Scale bars in j: retrosplenial cortex, 100 μm; hippocampus, 200 μm; NP tau, 25 μm. (l) Quantification of NP-tau-positive neurites as a percentage of AT8 staining within each NP, as represented in the right panels in j. Over 100 NP tau from three mice per group were examined. A two-tailed t-test was performed; *P ≤ 0.05, ***P ≤ 0.001. Data are presented as mean ± s.e.m. in b, i, k and l.

Journal: Nature medicine

Article Title: Amyloid-β plaques enhance Alzheimer’s brain tau-seeded pathologies by facilitating neuritic plaque tau aggregation

doi: 10.1038/nm.4443

Figure Lengend Snippet: Mislocalized tau in periplaque dystrophic axons is critical for AD-tau-induced NP tau aggregation. (a) Representative images of endogenous tau (red) detected by antibody K9JA in regions with or without Aβ plaques (blue) from 5xFAD mice with no endogenous tau ((Mapt−/− 5xFAD) (8 months)) or 5xFAD mice (8 months) injected with human control brain lysate or AD-tau at 3 m.p.i. Pathological tau (green) was detected by antibody AT8. Results from one out of three mice per group are shown. (b) Similar amounts of noncompact and compact plaques were present in control-5xFAD mice, and this ratio was not significantly altered in AD-5xFAD mice. (c) Proportion of each type of Aβ plaque surrounded by K9JA-labeled endogenous tau in control-5xFAD mice. Three mice were quantified. (d,e) Representative image (d) and proportion of each type (e) of Aβ plaque (red) surrounded by induced AT8-labeled NP tau (green) in AD-5xFAD mice. DAPI (blue) stains nucleus. In d, arrows indicate noncompact plaques and arrowheads indicate compact plaques. Scale bar in d, 100 μm. Quantifications in b, c and e were performed from the ipsilateral caudal hippocampal region of 5xFAD (8 months) mice injected with AD-tau or control at 3 m.p.i.; three mice per group were quantified. (f–i) Characterization of Mapt+/− 5xFAD mice (n = 3 per group). (f) PCR of genomic DNA confirming the genotype of Mapt+/− 5xFAD mice. The primers used for genotyping are indicated on the right. (g) Western blotting showing that endogenous mouse tau is reduced in Mapt+/− 5xFAD mice as compared to Mapt+/+ 5xFAD mice. (h) Representative NAB228 immunostaining; scale bar, 400 μm. (i) Quantification of brain slices containing caudal hippocampus showing the Aβ plaque burden in 8-month-old 5xFAD mice with or without tau reduction. Two-tailed t-tests were performed; *P ≤ 0.05. (j,k) Representative AT8 immunostaining (j) and quantification (k) of NP tau in both retrosplenial cortex (ctx) and hippocampus on the ipsilateral side of AD-tau-injected 8-month-old Mapt+/+ 5xFAD and Mapt+/− 5xFAD mice at 1 m.p.i. (n = 3 per group). A dashed circle in j indicates a single NP. Scale bars in j: retrosplenial cortex, 100 μm; hippocampus, 200 μm; NP tau, 25 μm. (l) Quantification of NP-tau-positive neurites as a percentage of AT8 staining within each NP, as represented in the right panels in j. Over 100 NP tau from three mice per group were examined. A two-tailed t-test was performed; *P ≤ 0.05, ***P ≤ 0.001. Data are presented as mean ± s.e.m. in b, i, k and l.

Article Snippet: Anti-tau antibodies R2295, Tau5 and K9JA were covalently conjugated to Dynabeads M-280 Tosylactivated (Fisher Scientific) per the manufacturer’s instructions and then mixed for use.

Techniques: Injection, Control, Labeling, Western Blot, Immunostaining, Two Tailed Test, Staining