Echelon Biosciences
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Merck & Co
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Image Search Results

Journal: Renal Failure
Article Title: HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in mice
doi: 10.1080/0886022X.2023.2188966
Figure Lengend Snippet: HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.
Article Snippet: Hyaluronidase activity was assessed by an
Techniques: Knockdown, Western Blot, Enzyme-linked Immunosorbent Assay, Activity Assay, Immunofluorescence, Staining, Fluorescence, Immunohistochemistry