junb Search Results


gene exp junb mm04243546 s1  (Thermo Fisher)
89
Thermo Fisher gene exp junb mm04243546 s1
Gene Exp Junb Mm04243546 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp junb mm04243546 s1/product/Thermo Fisher
Average 89 stars, based on 1 article reviews
gene exp junb mm04243546 s1 - by Bioz Stars, 2026-05
89/100 stars
  Buy from Supplier
anti junb ap 1  (Novus Biologicals)
94
Novus Biologicals anti junb ap 1
Anti Junb Ap 1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti junb ap 1/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
anti junb ap 1 - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
plasmid pmig backbone expressing junb  (Addgene inc)
86
Addgene inc plasmid pmig backbone expressing junb
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Plasmid Pmig Backbone Expressing Junb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid pmig backbone expressing junb/product/Addgene inc
Average 86 stars, based on 1 article reviews
plasmid pmig backbone expressing junb - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
junb  (Addgene inc)
93
Addgene inc junb
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Junb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/junb/product/Addgene inc
Average 93 stars, based on 1 article reviews
junb - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
ap 1  (Novus Biologicals)
93
Novus Biologicals ap 1
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Ap 1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ap 1/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
ap 1 - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
human ap n  (R&D Systems)
86
R&D Systems human ap n
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Human Ap N, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ap n/product/R&D Systems
Average 86 stars, based on 1 article reviews
human ap n - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
mfluor violet 450 se conjugated anti c jun  (Novus Biologicals)
94
Novus Biologicals mfluor violet 450 se conjugated anti c jun
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Mfluor Violet 450 Se Conjugated Anti C Jun, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mfluor violet 450 se conjugated anti c jun/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
mfluor violet 450 se conjugated anti c jun - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
gene exp junb hs00357891 s1  (Thermo Fisher)
96
Thermo Fisher gene exp junb hs00357891 s1
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Gene Exp Junb Hs00357891 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp junb hs00357891 s1/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
gene exp junb hs00357891 s1 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
4e bp1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc 4e bp1
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
4e Bp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4e bp1/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
4e bp1 - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier
monoclonal rabbit anti junb  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc monoclonal rabbit anti junb
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Monoclonal Rabbit Anti Junb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal rabbit anti junb/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
monoclonal rabbit anti junb - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier
gene exp junb mm00492781 s1  (Thermo Fisher)
96
Thermo Fisher gene exp junb mm00492781 s1
Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and <t>JunB</t> containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.
Gene Exp Junb Mm00492781 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp junb mm00492781 s1/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
gene exp junb mm00492781 s1 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
gene exp junb rn00572994 s1  (Thermo Fisher)
88
Thermo Fisher gene exp junb rn00572994 s1
a A schematic diagram showing the timeline for biochemistry and behavior experiments during chronic SSRI fluoxetine (Flx) treatment. Fluoxetine was administered orally to BALB/cJ mice for 28 days. Start of treatment indicated as Day 1 (red arrow). Behavioral analysis (black arrows) using open-field test (OFT) was performed on day 14 (Supplementary Fig. ), tail suspension test (TST) on day 16, and novelty suppressed feeding test (NSF) on day 18. Biochemistry was done after harvesting the mouse prefrontal cortex (PFC) on 2, 5, 9, 14, 21, and 28 days of treatment (blue arrows). The onset and maintenance of behavioral response is shaded in gray. b TST. c NSF. d – k Quantitative-polymerase chain reaction (qPCR) to measure the levels of transcription factor mRNA Creb1 ( d ), Crem ( e ), Egr1 ( f ), c-Fos ( g ), Fosl2 ( h ), c-Jun ( i ), <t>Junb</t> ( j ), Jund ( k ). l A schematic diagram describing the formation of the AP-1 complex. Extracellular signals activate c-Fos and c-Jun mRNA transcription and protein expression. The proteins get phosphorylated by kinases, forming a stable heterodimeric AP-1 complex, thereby binding to target DNA and controlling their transcription. m A schematic diagram depicting c-Fos and c-Jun regulation by chronic fluoxetine treatment. Statistical analysis was performed between vehicle- (1% saacharine in drinking water) and fluoxetine-treated samples using two-tailed unpaired Student’s t -test; n = 6 for biochemistry and n = 11-14 for behavioral experiments. Data are mean ± SEM; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.005.
Gene Exp Junb Rn00572994 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp junb rn00572994 s1/product/Thermo Fisher
Average 88 stars, based on 1 article reviews
gene exp junb rn00572994 s1 - by Bioz Stars, 2026-05
88/100 stars
  Buy from Supplier

Image Search Results


Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and JunB containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.

Journal: Immunity

Article Title: The IRF4 gene regulatory module functions as a read-write integrator to dynamically coordinate T helper cell fate

doi: 10.1016/j.immuni.2017.09.001

Figure Lengend Snippet: Irf4 targets distinct DNA motifs to control Tfh and Teff gene programs. ATAC-seq libraries were generated from the same cells in Figure 5. (A) Five patterns of differentially ChARs, A1-5, are shown as box plots (median ±%75th for the box and ±%25th for the whiskers) plotted as a function of averaged Z-scored values. The number of regions within each cluster are shown. (B) Peak tracks at the Bcl6 and Prdm1 loci from the indicated groups; CD4+ naïve and activated are from (GEO: GSE37074). ChARs from a given cluster are highlighted with a downward facing arrowhead and the parental cluster. Peaks with no arrows are not differentially accessible. (C) Heat map depicting the enrichment ratio (Log2) of given Irf4 DNA binding motifs (x-axis) in clusters A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). (D) Binding saturation curves of Irf4 to the AICE or ISRE motifs. Binding reactions using the AICE probe derived from Bcl11b were carried out in the presence of a constant amount of BATF and JunB containing nuclear extracts. Irf4 containing nuclear extract was increased in 2-fold increments as indicated. The ISRE probe from Prdm1 was used in binding reactions with Irf4 containing nuclear extracts as for the AICE reaction. Nuclear extracts containing a DNA-binding deficient Irf4 (R98A, C99A) were used at the highest concentration of the wild type Irf4 saturation curve as a specificity control. Arrows indicate complexes; AP-1 (blue), Irf4/AP-1 (red), and Irf4 homodimer (green); asterisk indicates non-specific binding. Densitometry analysis is shown to the right of the gel. Representative of two experiments performed. E) Heat map depicting the enrichment ratio of gene members from RNA clusters R1-6 (x-axis) in ChARs A1-5 (y-axis); inset text represents the enrichment p-value (Fisher’s Exact Test). See also Figure S7 and Tables S1–7.

Article Snippet: Plasmid: pMIG backbone expressing JunB , Addgene , Plasmid #40349.

Techniques: Control, Generated, Binding Assay, Derivative Assay, Concentration Assay

KEY RESOURCES TABLE

Journal: Immunity

Article Title: The IRF4 gene regulatory module functions as a read-write integrator to dynamically coordinate T helper cell fate

doi: 10.1016/j.immuni.2017.09.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Plasmid: pMIG backbone expressing JunB , Addgene , Plasmid #40349.

Techniques: Purification, Control, Recombinant, Transfection, Sensitive Assay, RNA HS Assay, Plasmid Preparation, Expressing, Mutagenesis, Software

a A schematic diagram showing the timeline for biochemistry and behavior experiments during chronic SSRI fluoxetine (Flx) treatment. Fluoxetine was administered orally to BALB/cJ mice for 28 days. Start of treatment indicated as Day 1 (red arrow). Behavioral analysis (black arrows) using open-field test (OFT) was performed on day 14 (Supplementary Fig. ), tail suspension test (TST) on day 16, and novelty suppressed feeding test (NSF) on day 18. Biochemistry was done after harvesting the mouse prefrontal cortex (PFC) on 2, 5, 9, 14, 21, and 28 days of treatment (blue arrows). The onset and maintenance of behavioral response is shaded in gray. b TST. c NSF. d – k Quantitative-polymerase chain reaction (qPCR) to measure the levels of transcription factor mRNA Creb1 ( d ), Crem ( e ), Egr1 ( f ), c-Fos ( g ), Fosl2 ( h ), c-Jun ( i ), Junb ( j ), Jund ( k ). l A schematic diagram describing the formation of the AP-1 complex. Extracellular signals activate c-Fos and c-Jun mRNA transcription and protein expression. The proteins get phosphorylated by kinases, forming a stable heterodimeric AP-1 complex, thereby binding to target DNA and controlling their transcription. m A schematic diagram depicting c-Fos and c-Jun regulation by chronic fluoxetine treatment. Statistical analysis was performed between vehicle- (1% saacharine in drinking water) and fluoxetine-treated samples using two-tailed unpaired Student’s t -test; n = 6 for biochemistry and n = 11-14 for behavioral experiments. Data are mean ± SEM; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.005.

Journal: Molecular Psychiatry

Article Title: AP-1 controls the p11-dependent antidepressant response

doi: 10.1038/s41380-020-0767-8

Figure Lengend Snippet: a A schematic diagram showing the timeline for biochemistry and behavior experiments during chronic SSRI fluoxetine (Flx) treatment. Fluoxetine was administered orally to BALB/cJ mice for 28 days. Start of treatment indicated as Day 1 (red arrow). Behavioral analysis (black arrows) using open-field test (OFT) was performed on day 14 (Supplementary Fig. ), tail suspension test (TST) on day 16, and novelty suppressed feeding test (NSF) on day 18. Biochemistry was done after harvesting the mouse prefrontal cortex (PFC) on 2, 5, 9, 14, 21, and 28 days of treatment (blue arrows). The onset and maintenance of behavioral response is shaded in gray. b TST. c NSF. d – k Quantitative-polymerase chain reaction (qPCR) to measure the levels of transcription factor mRNA Creb1 ( d ), Crem ( e ), Egr1 ( f ), c-Fos ( g ), Fosl2 ( h ), c-Jun ( i ), Junb ( j ), Jund ( k ). l A schematic diagram describing the formation of the AP-1 complex. Extracellular signals activate c-Fos and c-Jun mRNA transcription and protein expression. The proteins get phosphorylated by kinases, forming a stable heterodimeric AP-1 complex, thereby binding to target DNA and controlling their transcription. m A schematic diagram depicting c-Fos and c-Jun regulation by chronic fluoxetine treatment. Statistical analysis was performed between vehicle- (1% saacharine in drinking water) and fluoxetine-treated samples using two-tailed unpaired Student’s t -test; n = 6 for biochemistry and n = 11-14 for behavioral experiments. Data are mean ± SEM; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.005.

Article Snippet: Creb1 , Mm00501607_m1, Rn00578826_m1; Crem , Mm04336053_g1, Rn04338541_m1; Egr1 , Mm00656724_m1, Rn00561138_m1; c-Fos , Mm00487425_m1, Rn00487426_g1; Fosl2 , Mm00484442_m1, Rn00564121_m1; Fosl1 , Mm04207958_m1, Rn00564121_m1; Fosb , Mm00500401_m1, Rn00500401_m1; c-Jun , Mm00495062_s1, Rn99999045_s1; Junb , Mm04243546_s1 Rn00572994_s1; Jund , Mm04208316_s1, Rn00824678_s1; S100a10 , Mm00501457_m1, Rn01409218_m1; Bdnf , Mm04230607_s1, Rn02531967_s1; Fgf2 , Mm00433287_m1, Rn00570809_m1; Egf , Mm00438696_m1, Rn00563336_m1; Igf , Mm00439560_m1, Rn00710306_m1; Ngf , Mm00443039_m1, Rn01533872_m1; Vegf , Mm00437306_m1, Rn01511602_m1; Tgfβ , Mm01178820_m1, Rn00572010_m1; Gapdh , Mm_99999915_g1, Rn99999916_s1; Srsf5 , Mm00833629_g1; Slc1a2 , Mm01275814_m1; Sirt1 , Mm01168521_m1; Glul , Mm00725701_s1; Glo1 , Mm00844954_s1; Crhr1 , Mm00432670_m1; Adrb1 , Mm00431701_s1; Abcb1 , Mm00440736_m1.

Techniques: Suspension, Real-time Polymerase Chain Reaction, Expressing, Binding Assay, Two Tailed Test

Identification of transcription factor(s) regulating p11 by RNAi.

Journal: Molecular Psychiatry

Article Title: AP-1 controls the p11-dependent antidepressant response

doi: 10.1038/s41380-020-0767-8

Figure Lengend Snippet: Identification of transcription factor(s) regulating p11 by RNAi.

Article Snippet: Creb1 , Mm00501607_m1, Rn00578826_m1; Crem , Mm04336053_g1, Rn04338541_m1; Egr1 , Mm00656724_m1, Rn00561138_m1; c-Fos , Mm00487425_m1, Rn00487426_g1; Fosl2 , Mm00484442_m1, Rn00564121_m1; Fosl1 , Mm04207958_m1, Rn00564121_m1; Fosb , Mm00500401_m1, Rn00500401_m1; c-Jun , Mm00495062_s1, Rn99999045_s1; Junb , Mm04243546_s1 Rn00572994_s1; Jund , Mm04208316_s1, Rn00824678_s1; S100a10 , Mm00501457_m1, Rn01409218_m1; Bdnf , Mm04230607_s1, Rn02531967_s1; Fgf2 , Mm00433287_m1, Rn00570809_m1; Egf , Mm00438696_m1, Rn00563336_m1; Igf , Mm00439560_m1, Rn00710306_m1; Ngf , Mm00443039_m1, Rn01533872_m1; Vegf , Mm00437306_m1, Rn01511602_m1; Tgfβ , Mm01178820_m1, Rn00572010_m1; Gapdh , Mm_99999915_g1, Rn99999916_s1; Srsf5 , Mm00833629_g1; Slc1a2 , Mm01275814_m1; Sirt1 , Mm01168521_m1; Glul , Mm00725701_s1; Glo1 , Mm00844954_s1; Crhr1 , Mm00432670_m1; Adrb1 , Mm00431701_s1; Abcb1 , Mm00440736_m1.

Techniques: