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Westburg bv
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Avantor
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Image Search Results
Journal: Scientific Reports
Article Title: Control of cortex development by ULK4, a rare risk gene for mental disorders including schizophrenia
doi: 10.1038/srep31126
Figure Lengend Snippet: ( A ) Validation of Ulk4 R cDNA. Panels of plasmids containing different DNA constructs were co-transfected into HEK293 cells. Western blotting with anti-c-Myc (top row) and anti-flag (mid-row) indicated that Ulk4 R cDNA successfully restores the expression of Ulk4. Lane 1: co-transfection with plasmids containing control shRNA and Ulk4 cDNA; Lane 2: co-transfection with Ulk4 cDNA and shRNA268; Lane 3: co-transfection with control shRNA and Ulk4 R cDNA; Lane 4: co-transfection with plasmids containing shRNA268 and Ulk4 R cDNA. ( B–D ) Compared with the control group ( B ), the defective radial migration caused by shRNA268 ( C ) can be successfully rescued by Ulk4 R cDNA ( D ). Bars = 100 μm in ( B – D ).
Article Snippet: To determine the knockdown efficiency of Ulk4 shRNAs, cells at 80% confluence were transfected with plasmids containing either control (1 μg) or Ulk4 shRNAs (268 or 269, 1 μg/each) combined with full length mouse Ulk4 cDNA (1 μg) using
Techniques: Biomarker Discovery, Construct, Transfection, Western Blot, Expressing, Cotransfection, Control, shRNA, Migration
Journal: Italian Journal of Pediatrics
Article Title: The role of miR-29c/B7-H3/Th17 axis in children with Mycoplasma pneumoniae pneumonia
doi: 10.1186/s13052-019-0655-5
Figure Lengend Snippet: Transfection of miR-29c and B7-H3 detection by immunofluorescence staining. Comparison of grey levels of B7-H3 expression using Image-Pro Plus software. Data are presented as mean ± SEM of 3 independent experiments. ** P < 0.01; *** P < 0.001
Article Snippet: THP-1 cells (ATCC, Manassas, VA, USA) were seeded at 1 × 10 5 cells/60 mm dishes and then transfected with 100 nM pLenti-miR-29c, anti-miR-29c or empty vector using the
Techniques: Transfection, Immunofluorescence, Staining, Expressing, Software