Journal: Applied and Environmental Microbiology

Article Title: Enterocin F4-9, a Novel O -Linked Glycosylated Bacteriocin

doi: 10.1128/AEM.00940-15

Figure Lengend Snippet: Effect of enterocin F4-9 on E. faecalis JCM 5803T growth. Enterocin F4-9 at final concentrations of 0 μM (circles), 3 μM (triangles), and 40 μM (squares) were added to the cultures of the indicator strain, E. faecalis JCM 5803T. Values are means ± standard deviations (error bars) derived from triplicate experiments.

Article Snippet: In keeping with other bacteriocin-producing bacteria, E. faecalis F4-9 exhibits self-immunity against enterocin F4-9. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Antimicrobial spectrum of the purified enterocin F4-9 Open in a separate window a JCM, Japan Collection of Microorganisms (Wako, Japan); ATCC, American Type Culture Collection (Manassas, VA); NBRC, National Institute of Technology and Evaluation Biological Resource Center (Chiba, Japan). b NA, no activity (>30 μM). c Epicentre (Madison, WI). d Promega (Madison, WI). e GE Healthcare (Uppsala, Sweden).

Techniques: Derivative Assay

Journal: Applied and Environmental Microbiology

Article Title: Enterocin F4-9, a Novel O -Linked Glycosylated Bacteriocin

doi: 10.1128/AEM.00940-15

Figure Lengend Snippet: Experimental and theoretical masses of the native and chemically modified enterocin F4-9

Article Snippet: In keeping with other bacteriocin-producing bacteria, E. faecalis F4-9 exhibits self-immunity against enterocin F4-9. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Antimicrobial spectrum of the purified enterocin F4-9 Open in a separate window a JCM, Japan Collection of Microorganisms (Wako, Japan); ATCC, American Type Culture Collection (Manassas, VA); NBRC, National Institute of Technology and Evaluation Biological Resource Center (Chiba, Japan). b NA, no activity (>30 μM). c Epicentre (Madison, WI). d Promega (Madison, WI). e GE Healthcare (Uppsala, Sweden).

Techniques: Modification, Activity Assay

Journal: Applied and Environmental Microbiology

Article Title: Enterocin F4-9, a Novel O -Linked Glycosylated Bacteriocin

doi: 10.1128/AEM.00940-15

Figure Lengend Snippet: Antimicrobial activity assay of the native (a), reduced and alkylated (b), and deglycosylated (c) enterocin F4-9. The antimicrobial properties of each sample were assessed by its ability to inhibit the growth of E. faecalis JCM 5803T. As indicated, the reduced and alkylated peptide and the deglycosylated peptide did not exhibit antimicrobial activities.

Article Snippet: In keeping with other bacteriocin-producing bacteria, E. faecalis F4-9 exhibits self-immunity against enterocin F4-9. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Antimicrobial spectrum of the purified enterocin F4-9 Open in a separate window a JCM, Japan Collection of Microorganisms (Wako, Japan); ATCC, American Type Culture Collection (Manassas, VA); NBRC, National Institute of Technology and Evaluation Biological Resource Center (Chiba, Japan). b NA, no activity (>30 μM). c Epicentre (Madison, WI). d Promega (Madison, WI). e GE Healthcare (Uppsala, Sweden).

Techniques: Activity Assay