j774a Search Results


murine macrophage cell line j774a 1  (ATCC)
99
ATCC murine macrophage cell line j774a 1
Murine Macrophage Cell Line J774a 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/J774A%2E1/pm24729189-167-1-10
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j774a  (CLS Cell Lines Service GmbH)
93
CLS Cell Lines Service GmbH j774a
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/J774A%2E1+Cells/pmc11657075-169-33-39
Average 93 stars, based on 1 article reviews
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macrophages cell line j774a  (DSMZ)
95
DSMZ macrophages cell line j774a
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Macrophages Cell Line J774a, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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macrophages cell line j774a - by Bioz Stars, 2026-10
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j774 mouse macrophages  (ATCC)
97
ATCC j774 mouse macrophages
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774 Mouse Macrophages, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/J774A%2E1%3B+Monocyte-macrophage%3B+Mouse/pmc00097382-231-38-41
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1vl rrid cvcl 0493 mouse j774a 1 atcc cat tib 67  (AcceGen Biotechnology)
93
AcceGen Biotechnology 1vl rrid cvcl 0493 mouse j774a 1 atcc cat tib 67
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
1vl Rrid Cvcl 0493 Mouse J774a 1 Atcc Cat Tib 67, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/J774A%2E1/pm40580479-250-13-24
Average 93 stars, based on 1 article reviews
1vl rrid cvcl 0493 mouse j774a 1 atcc cat tib 67 - by Bioz Stars, 2026-10
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raw264.7 cell line  (JCRB Cell Bank)
90
JCRB Cell Bank raw264.7 cell line
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Raw264.7 Cell Line, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/j774a+1+cells/pmc11204705-128-0-20
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raw264.7 cell line - by Bioz Stars, 2026-10
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j774a.1 mouse mf-like cells  (Verlag GmbH)
90
Verlag GmbH j774a.1 mouse mf-like cells
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a.1 Mouse Mf Like Cells, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/j774a+1+mouse+mf+like+cells/pm19130551-61-11-17
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j774a.1 macrophage cell line  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection j774a.1 macrophage cell line
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a.1 Macrophage Cell Line, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/j774a+1+murine+macrophage+cell+line/pmc09654823-94-1-7
Average 90 stars, based on 1 article reviews
j774a.1 macrophage cell line - by Bioz Stars, 2026-10
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mouse macrophages j774a.1  (National Centre for Cell Science)
90
National Centre for Cell Science mouse macrophages j774a.1
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Mouse Macrophages J774a.1, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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j774 a-1  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies j774 a-1
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774 A 1, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/j774a/j774+a+1/pm11566193-27-0-9
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murine macrophages j774a.1  (LGC Promochem)
90
LGC Promochem murine macrophages j774a.1
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
Murine Macrophages J774a.1, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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j774a.1 cells  (Biochrom)
90
Biochrom j774a.1 cells
Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages <t>(J774A.1)</t> and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.
J774a.1 Cells, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages (J774A.1) and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Nanoporous Submicron Gold Particles Enable Nanoparticle‐Based Localization Optoacoustic Tomography (nanoLOT)

doi: 10.1002/smll.202404904

Figure Lengend Snippet: Biocompatibility and biosafety of PEGylated gold yarn balls (AuYB). A) Schematic representation of the alamarBlue cell metabolism assay steps: the cells are incubated with AuYB40‐PEG for 24 h and then with alamarBlue (aB) for 4 h, followed by readouts at 540 nm excitation and 580 nm emission wavelength. b) Results of alamarBlue cell viability assay conducted on a Chinese hamster ovary (CHO), murine macrophages (J774A.1) and human umbilical vein endothelial cells (HUVEC) cell cultures fed with different concentrations of AuYB40‐PEG. c) Schematic representation of the cell membrane damage test: the cells were first incubated with AuYB40‐PEG, then with aB to highlight the cytoplasm and sequentially with PBS, DAPI, or Triton X‐100 (TX100) + DAPI. d) Confocal microscopy images of the HUVEC cell culture incubated with aB + TX100 + DAPI, PBS + aB + DAPI, AuYB40‐PEG + aB + DAPI. The blue color indicates the DAPI signal and violet color indicates the aB signal. Scalebar − 100 µm. g) Murine blood biochemistry taken on the 11th day post‐injection of AuYB40‐PEG nanoparticles or phosphate‐buffered saline (PBS, control group): ALB – albumin, ALP – alkaline phosphatase, ALT – alanine transaminase, AMY – amylase, TBIL – total bilirubin, BUN – blood urea nitrogen, CA – calcium, PHOS – phosphates, GLU – glutamine, Na+ – sodium, K+ – potassium, TP – total protein, GLOB –globulin). h) Immune cell content of murine blood collected on the 11th days post‐injection of AuYB40 nanoparticles or PBS (control group): WBC – white blood cells in total, Neu – neutrophils, Lym – lymphocytes, Mon – monocytes, Eos – eosinophils.

Article Snippet: Dulbecco's Modified Eagle's Medium (DMEM), alamarBlue cell assay kit, 4′,6‐diamidino‐2‐phenylindole (DAPI), fetal bovine serum (FBS), 6‐well plates and 96‐well plates were purchased from Thermo Fisher Scientific Inc. Chinese hamster ovary (CHO) cells and J774A.1 murine macrophages were obtained from CLS Cell Lines Service GmbH.

Techniques: Incubation, Viability Assay, Membrane, Confocal Microscopy, Cell Culture, Injection, Saline, Control