Journal: Chinese Medicine

Article Title: Astragalus membranaceus (Huangqi) and Rhizoma curcumae (Ezhu) decoction suppresses colorectal cancer via downregulation of Wnt5/β-Catenin signal

doi: 10.1186/s13020-021-00564-6

Figure Lengend Snippet: Wnt5 or β-Catenin inhibitor promotes the inhibition ability of AR-CS in proliferation and migration of SW620 cells. SW620 cells were treated with or without 20% AR-CS, Wnt5 inhibitor (IWP-4), and β-Catenin inhibitor (JW55) treated for 24 h, real-time qPCR was used to detect Wnt5 ( A ), β-Catenin ( B ), ARF6 ( C ), and N-Cadherin ( D ) mRNA expression. Western blot was used to detect Wnt5, β-Catenin ( E ), ARF6, and N-Cadherin ( F ) protein expression. Wnt5 ( G ), β-Catenin ( H ), ARF6 ( I ), and N-Cadherin ( J ) protein expression level was normalized to control group. Transwell assay ( K ) was used to detect cell migration rate ( L ). Wound scratch assay ( M ) was used to detect cell wound healing rate ( N ). Immunofluorescent staining was used to detect β-Catenin expression ( O ) and the fluorescence density was calculated ( P ). Scale bar = 100 µm in K , Scale bar = 400 µm in M , Scale bar = 20 µm in O . N = 6, in indicating comparison, *p < 0.05

Article Snippet: IWP-4 (#HY-12879) and JW55 (#HY-13968) were purchased from MedChemExpress (New Jersey, USA).

Techniques: Inhibition, Migration, Expressing, Western Blot, Control, Transwell Assay, Wound Healing Assay, Staining, Fluorescence, Comparison