Journal: Journal of Assisted Reproduction and Genetics

Article Title: Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes

doi: 10.1007/s10815-024-03143-4

Figure Lengend Snippet: Treatment with OSC-IVM improves maturation rate of human denuded oocytes compared to an IVM media-matched control. A Schematic of the experimental co-culture IVM approach. hiPSCs were differentiated using inducible transcription factor overexpression to form ovarian support cells (OSCs). Human oocytes were obtained from patients in the clinic after standard gonadotropin stimulation, and immature oocytes (GV and MI) identified after denuding were allocated between the experimental OSC-IVM condition (OSC-IVM) or the control IVM media condition (Media-IVM) for IVM co-culture. Oocyte maturation and health were assessed after 24–28 h IVM co-culture, and oocytes were frozen for further analyses. The figure was created with BioRender.com. B Representative image of co-culture containing immature human oocytes ( n = 3) and OSCs. Scale bar: 200 µm. Denuded GV oocytes are seen with surrounding OSCs in suspension culture. C Maturation rate of oocytes after 24–28 h IVM experiments, including oocyte co-culture with OSCs (OSC-IVM), or in media control (Media-IVM). n indicates the number of individual oocytes in each culture condition. Error bars indicate mean ± SEM. The p -value is derived from unpaired t -test comparing experimental OSC-IVM to control Media-IVM. Due to low numbers of retrieved oocytes per donor, each group contains oocytes from predominantly non-overlapping donor groups, and pairwise comparisons are not utilized

Article Snippet: All donated immature oocytes were maintained in preincubation LAG Medium (Medicult, Cooper Surgical) at 37 °C for 2–3 h after cumulus removal prior to introduction to in vitro maturation conditions for 24 to 28 h (either Media-IVM or OSC-IVM).

Techniques: Control, Co-Culture Assay, Over Expression, Suspension, Derivative Assay

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes

doi: 10.1007/s10815-024-03143-4

Figure Lengend Snippet: Cell culture media conditions

Article Snippet: All donated immature oocytes were maintained in preincubation LAG Medium (Medicult, Cooper Surgical) at 37 °C for 2–3 h after cumulus removal prior to introduction to in vitro maturation conditions for 24 to 28 h (either Media-IVM or OSC-IVM).

Techniques: Cell Culture, Recombinant

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes

doi: 10.1007/s10815-024-03143-4

Figure Lengend Snippet: MII oocytes treated with OSC-IVM are enriched for genes related to oocyte maturation and embryo development. A Graphical illustration of the strategy to identify transcriptomic profile enrichment during the progression from the germinal vesicle (GV) to MII oocyte. Differentially expressed genes (DEG) were identified by comparing GVs to MII oocytes. B Venn diagram illustrating the gene enrichment in successfully matured MII versus fail-to-mature GVs treated with OSC-IVM versus Media-IVM oocytes. Numbers display the total number of genes identified. Selected functional enrichment analysis terms are shown for each subgroup. See Supplementary Fig. for extended details. C Venn diagram illustrating the gene depletion in successfully matured MII versus fail-to-mature GVs treated with OSC-IVM versus Media-IVM oocytes. Numbers display the total number of genes identified. Selected functional enrichment analysis terms are shown for each subgroup. See Supplementary Fig. for extended details

Article Snippet: All donated immature oocytes were maintained in preincubation LAG Medium (Medicult, Cooper Surgical) at 37 °C for 2–3 h after cumulus removal prior to introduction to in vitro maturation conditions for 24 to 28 h (either Media-IVM or OSC-IVM).

Techniques: Functional Assay

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes

doi: 10.1007/s10815-024-03143-4

Figure Lengend Snippet: Transcriptomics analysis reveals that oocytes matured under the OSC-IVM condition are transcriptionally closer to IVF MII oocytes than those oocytes matured in Media-IVM, despite no changes in morphological features. A Total Oocyte Scores (TOS) generated from imaging analysis of MII oocytes after 24–28 h IVM experiments and IVF-MII oocytes. n indicates the number of individual MII oocytes analyzed. Median (dashed lines) and quartiles (dotted lines) are indicated. ANOVA indicated no significant ( p = 0.5274) difference between the means of each condition. B Quantification of the angle between the first polar body (PB1) and spindle apparatus, derived from oocyte fluorescent imaging analysis (See Supplementary Fig. ), of oocytes co-cultured with OSCs (OSC-IVM) or in media control (Media-IVM), and IVF-MII oocytes. n indicates the number of individual oocytes analyzed from each condition. Number and percentage (%) of MII oocytes with no spindle assembly observed are also indicated below the axis labels in the dashed box. Median (dashed line) and quartiles (dotted line) are indicated. ANOVA statistical analysis found no significant difference (ns, p = 0.1155) between the means of each condition. C Scatterplot projections of oocyte transcriptomes generated from the GV fail-to-mature Signature Score (X axis) and IVF MII Signature Score (Y axis). Symbols are color-coded based on the experimental condition (OSC-IVM, Media-IVM, IVF-MII), and symbol shapes represent oocyte maturation stages (GV, MI, and MII). Each symbol represents one oocyte. Histograms on top depict distribution of MII oocytes across the GV fail-to-mature Signature Score axis. Histograms on the right depict distribution of MII oocytes across the IVF MII Signature Score axis. Histograms are color-coded based on the experimental condition. n = 114 oocytes. D BoxPlot of distribution of MII oocytes across IVF MII Signature Score (left) and GV fail-to-mature Signature Score (right). ANOVA statistical analysis found a significant difference between the OSC-IVM-MII oocytes and Media-IVM MII oocytes (** p = 0.0003) and between the IVF-MII oocytes and Media-IVM oocytes (**** p < 0.0001), as well as between the OSC-IVM-MII oocytes and the IVF-MII oocytes (** p < 0.0026)

Article Snippet: All donated immature oocytes were maintained in preincubation LAG Medium (Medicult, Cooper Surgical) at 37 °C for 2–3 h after cumulus removal prior to introduction to in vitro maturation conditions for 24 to 28 h (either Media-IVM or OSC-IVM).

Techniques: Generated, Imaging, Derivative Assay, Cell Culture, Control

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes

doi: 10.1007/s10815-024-03143-4

Figure Lengend Snippet: Pathway enrichment analysis reveals similarities between MII oocytes rescued from OSC-IVM and IVF-MII oocytes. A Dotplot displaying gene enrichment among Media-IVM MII oocytes, OSC-IVM MII oocytes, and IVF-MII oocytes. The bottom panel shows enrichment of Gene Ontology (GO) terms and KEGG and REACTOME pathways (see an extended version in Supplementary Fig. ). GO, gene ontology; MF, molecular function; BP, biological process; CC, cellular component; KEGG, KEGG pathway; REAC, Reactome pathway. B Heatmap of Gene Set Enrichment Analysis (GSEA) hallmarks among Media-IVM MII oocytes, OSC-IVM MII oocytes, and IVF-MII oocytes. Heatmap represents row-normalized gene expression using a color gradients scale ranging from higher (red) to lower (blue) relative levels

Article Snippet: All donated immature oocytes were maintained in preincubation LAG Medium (Medicult, Cooper Surgical) at 37 °C for 2–3 h after cumulus removal prior to introduction to in vitro maturation conditions for 24 to 28 h (either Media-IVM or OSC-IVM).

Techniques: Expressing

Journal: Cureus

Article Title: Successful Pregnancy Following Platelet-Rich Plasma Intraovarian Injection and In Vitro Maturation of Oocytes in a 47-Year-Old Woman: A Case Report

doi: 10.7759/cureus.65281

Figure Lengend Snippet: (a) The COCs at egg retrieval (day 0), showing signs of immaturity. (b) The first COC 23 hours post-IVM before denudation, showing a first polar body, indicated by a white arrow (mature MII oocyte). (c) The second COC 23 hours post-IVM being immature at the GV stage. (d) The oocyte after denudation, with the first polar body at a 12-hour position before ICSI. (e) Normal fertilization indicated by the presence of two pronuclei 16 hours post-ICSI. (f) Development of the fertilized oocyte to the blastocyst stage. IVM, in vitro maturation; ICSI, intracytoplasmic sperm injection; COC, cumulus-oocyte complex; MII, metaphase II; GV, germinal vesicle

Article Snippet: The COCs were immature at oocyte retrieval (day 0), as expected, and they were subsequently cultured in LAG medium (Origio, Malov, Denmark) for three hours, followed by culture in IVM medium (Origio) for 22 hours in 6% CO 2 /7% O 2 at 37°C (Figure ).

Techniques: In Vitro, Injection

Journal: Frontiers in Endocrinology

Article Title: Immature Oocyte for Fertility Preservation

doi: 10.3389/fendo.2019.00464

Figure Lengend Snippet: Summary of IVM oocyte vitrification studies.

Article Snippet: Recently, commercialized IVM media such as SAGE (Coopersurgical) IVM medium and Medi-Cult IVM medium have been used in several IVF centers as they have the advantage of being certified as IVF quality controlled ( – ).

Techniques: In Vivo, In Vitro, Activation Assay